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作物学报 ›› 2010, Vol. 36 ›› Issue (1): 36-46.doi: 10.3724/SP.J.1006.2010.00036

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆重组自交系群体NJRIKY遗传图谱的加密及其应用效果

周斌1,邢邯1,陈受宜2，盖钧镒1   

  1. 1南京农业大学大豆研究所/国家大豆改良中心/作物遗传生理重点实验室，南京210095；2中国科学院遗传与发育生物学研究所植物基因组学国家重点实验室，北京100101
  • 收稿日期:2009-05-08 修回日期:2009-10-02 出版日期:2010-01-12 网络出版日期:2009-11-17
  • 通讯作者: 盖钧镒, E-mail: sri@njau.edu.cn; Tel: 025-84395405
  • 基金资助:

    本研究由国家重点基础研究发展计划（973计划）项目（2006CB101708,2009CB118404），国家高新技术研究发展计划（863计划）项目（2006AA100104），教育部高等学校学科创新引智计划项目（B08025），农业部公益性行业专项（200803060）资助。

Density-Enhanced Genetic Linkage Map of RIL Population NJRIKY and Its Impacts on Mapping Genes and QTLs and QTLs in Soybean

ZHOU Bin1,XING Han1,CHEN Shou-Yi2,GAI Jun-Yi1,*   

  1. 1Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvemet/State Key Laboratory of Crop Genetics and Germplasm Enhancement， Nanjing 210095，China；2 State Key Laboratory of Plant Gemomics，Institute of Genetics and developmental Biology,Chinese Academy of Sciences,Being 100101,China
  • Received:2009-05-08 Revised:2009-10-02 Published:2010-01-12 Published online:2009-11-17
  • Contact: Gai Jun-Yi, E-mail: sri@njau.edu.cn; Tel: 025-84395405

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1839

被引次数

21

摘要：

作物基因组研究，包括基因或数量性状位点(QTL)定位、图位克隆以及物理图谱构建等，首先必须建立具有丰富标记信息的高密度遗传连锁图谱。由科丰1号和南农1138-2杂交组合衍生的重组自交系群体NJRIKY已经构建了4张大豆遗传连锁图谱，但由于遗传信息和标记数目不够充分，在基因和QTL作图时仍然存在精确度和准确度问题。为增加NJRIKY图谱密度，本研究在967对SSR引物中获得了401个多态性SSR标记。结合其他分子数据，使用作图软件Mapmaker/exp 3.0b，获得一张含有553个遗传标记，25个连锁群，总长2 071.6 cM，平均图距3.70 cM的新遗传连锁图谱，其中SSR标记316个，RFLP标记197个，EST标记39个，形态标记1个。连锁群上大于20 cM的标记间隔由原来42个减少到2个。原图谱的3个SMV抗性基因定位于D1b连锁群末端的开放区间上且仅与一个RFLP标记连锁，利用加密图谱对Rsc-3、Rsc-7、Rsc-9、Rsc-13、Rsa、Rn1和Rn3等7个SMV抗性基因重定位，全部位于D1b连锁群，与相邻分子标记距离均小于6 cM，其中Rsc-9、Rn1、Rsa的距离小于1 cM，Rsc-13与EST标记GMKF168a共分离。对本群体农艺性状进行QTL重定位，获得8个性状相关的42个主效QTL，其中20个QTL遗传贡献率大于10%，与原图谱比较，新定位的各QTL的标记区间明显缩短，与相邻标记的连锁更加紧密。

关键词: 大豆, 遗传连锁图谱, SSR标记, QTL

Abstract:

A high-density genetic linkage map with informative markers is essential for plant genome analysis, including gene mapping, identification of quantitative trait locus (QTL), map-based cloning, and physical map construction. Though four genetic linkage maps for the recombinant inbred line population NJRIKY derived from (Kefeng 1×Nannong 1138-2) have been established already, there are still problems of precision and accuracy in mapping genes and QTLs due to insufficiency of genetic information and number of markers. A total of 401 polymorphic SSR markers were screened out from 967 ones for density-enhancement of the previous maps. Along with other marker data, a new genetic linkage map was constructed by using Mapmaker/Exp 3.0b, with 553 markers, including 316 SSR, 197 RFLP, 39 EST and one morphologic markers, spanning 25 linkage groups, covering total length 2 071.6 cM of the soybean genome, with an average marker interval distance of 3.70 cM. In comparison with the old map, the number of gaps larger than 20 cM decreased from forty-two to two on the enhanced map. Using this map to relocate the seven SMV resistant genes, Rsc-3, Rsc-7, Rsc-9, Rsc-13, Rsa, Rn1 and Rn3 were mapped on LG D1b again with distances to the flanking markers all less than 6 cM, among them, Rsc-9, Rn1 and Rsa less than 1 cM and Rsc-13 co-segregating with EST-SSR marker GMKF168a. After re-mapping the QTLs for eight agronomic traits, 42 QTLs were detected on 12 linkage groups, with 20 of them accounted for more than 10% of the total variation, respectively, and their marker intervals obviously shortened.

Key words: Soybean, Genetic linkage map, SSR markers, QTL

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