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作物学报 ›› 2010, Vol. 36 ›› Issue (07): 1108-1113.doi: 10.3724/SP.J.1006.2010.01108

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻中胚轴长度QTL分析

黄成,姜树坤**,冯玲玲,徐正进*,陈温福   

  1. 沈阳农业大学/农业部作物生理生态遗传育种重点开放实验室/辽宁省北方粳稻育种重点实验室，辽宁沈阳110161
  • 收稿日期:2010-02-03 修回日期:2010-04-20 出版日期:2010-07-12 网络出版日期:2010-06-12
  • 基金资助:

    本研究由国家自然科学基金项目（30971845）和国家重点基础研究发展计划（973计划）项目（2009CB126007）资助。

QTL Analysis for Mesocotyl Length in Rice(Qryza sativa L.)

HUANG Cheng,JIANG Shu-Kun**,FENG Ling-Ling,XU Zheng-Jin*,CHEN Wen-Fu   

  1. Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture / Key Laboratory of Northern Japonica Rice Breeding of Liaoning Province / Shenyang Agricultural University, Shenyang 110161, China
  • Received:2010-02-03 Revised:2010-04-20 Published:2010-07-12 Published online:2010-06-12

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2369

被引次数

15

摘要：

为分析水稻中胚轴伸长与赤霉素的关系及其遗传基础, 以沈农265 (长中胚轴)和丽江新团黑谷(短中胚轴)的RIL群体为材料, 结合其连锁图谱, 对水和赤霉素溶液两种培养条件下的中胚轴长度进行QTL定位。结果表明, 浓度为1.50 mmol L-1的赤霉素可显著促进中胚轴的伸长。两种培养条件下, 共检测到控制中胚轴长度的5个QTL, 分布在第1、第2、第3、第6和第11号染色体上, LOD值在3.65~15.52范围内, 单个QTL对表型贡献率在7%~33%之间。其中qML3、qML6和qML11在2种处理条件下均被检测到, qML1和qML2仅在水培条件下被检测到。与其他研究比较发现, 主效基因qML3可以在不同群体和不同环境下稳定表达。

关键词: 水稻, 中胚轴长度, 赤霉素, 数量性状座位

Abstract:

To understand genetic basis underlying the relationship between mesocotyl elongation and hormone (GA) in rice (Oryza sativa L.), we genetically analyzed RILs population derived from a cross between two japonica rice cultivars, Shennong 265 (long mesocotyl) and Lijiangxintuanheigu (short mesocotyl), with its linkage map. QTLs for mesocotyl length were detected under water and GA germination conditions. The results showed that GA at 1.50 mmol L-1 stimulated mesocotyl elongation dramatically. Total five QTLs for rice mesocotyl length, each accounting for 7% to 33% of the phenotypic variance (VE), were detected on chromosomes 1, 2, 3, 6, and 11 under two germination conditions (environments). The LOD value of each QTL was ranged from 3.65 to 15.52. Among the five QTLs, qML3, qML6, and qML11 were detected under both germination conditions and qml1 and qml2 were identified only under water germination condition. In comparison with other studies revealed that major QTL qML3 was easily re-detected in different populations and environments.

Key words: Rice, Mesocotyl length, Gibberellin, Quantitative trait loci

  
[1]    Zheng X-R(郑相如), Fan Y-L(范雅兰). Hypocotyl—a special strucyure of embryophyte. Biol Bull (植物学通报), 1998, 33(5): 10-11 (in Chinese)
  

[2]    Wu M G, Zhang G H, Lin J R, Cheng S H. Screening for rice germplasms with specially-elongated mesocotyl. Rice Sci, 2005, 12(3): 226-228
  

[3]    Wang Y(王莹), Ma D-R(马殿荣), Chen W-F(陈温福). Pilot study on mesocotyl elongation characters of northern weedy rice. China Rice (中国稻米), 2008, (3): 47-49 (in Chinese)
  

[4]    Lin J-R(林建荣), Zhang G-H(张光恒), Wu M-G(吴明国), Cao L-Y(曹立勇), Cheng S-H(程式华). Genetic analysis of mesocotyl elongation in rice (Oryza sativa L. subsp. japanica). Acta Agron Sin (作物学报), 2006, 32(2): 249-252 (in Chinese with English abstract)
  

[5]    Redoña E D, Mackill D J. Mapping quantitative trait loci for seedling vigor in rice using RFLP. Theor Appl Genet, 1996, 92: 395-402
  

[6]    Cao L-Y(曹立勇), Zhu J(朱军), Yan Q-C(颜启传), He L-B(何立斌), Wei X-H(魏兴华), Cheng S-H(程式华). Mapping QTLs with epistasis for mesocotyl length in a DH population from indica-japanica cross of rice (Oryza sativa). Chin J Rice Sci (中国水稻科学), 2002, 16(3): 221-224 (in Chinese with English abstract)
  

[7]    Katsuta-Seki M, Ebana K, Okuno K. QTL analysis for mesocotyl elongation in rice. Rice Genet Newsl, 1996, 13: 126-127
  

[8]    Nishizawa T, Suge H. Ethylene and carbon dioxide: regulation of oat mesocotyl growth. Plant Cell Environ, 1995, 18: 197-203
  

[9]    Suge H. Mesocotyl elongation in japonica rice: effect of high temperature pre-treatment and ethylene. Plant Cell Physiol, 1972, 13: 401-405


[10]    Chen J-G(陈金桂), Zhang Y-Z(张玉宗), Zhou X(周燮). Regulation foxtail millet mesocotyl growth gibberellins and abscisic acid in etiolated seedlings. J Nanjing Agric Univ (南京农业大学学报), 1997, 20(1): 13-17 (in Chinese with English abstract)


[11]    Cao L-Y(曹立勇), Yuan S-J(袁守江), Zhou H-P(周海鹏), Zhan X-D(占小登), Wu W-M(吴伟明), Gao J-X(高俊贤), Cheng S-H(程式华). Effect of different hormones on mesocotyl length in Oryza sativa L. Acta Agron Sin (作物学报), 2005, 31(8): 1098-1100 (in Chinese with English abstract)


[12]    Jiang S-K(姜树坤), Zhang X-J(张喜娟), Xu Z-J(徐正进), Chen W-F(陈温福). Comparison between QTLs for chlorophyll content and genes controlling chlorophyll biosynthesis and degradation in japonica rice (Oryza sativa L.). Acta Agron Sin (作物学报), 2010, 36(3): 376-384 (in Chinese with English abstract)


[13]    Nelson J C. QGENE: software for marker-based genomic analysis and breeding. Mol Breed, 1997, 3: 239-245


[14]    Wu S-Q(吴三桥), Ding R(丁锐), Li X-S(李新生). Regulation of mesocotyl growth by gibberellic acid and abscisic acid in etiolated seedlings of black rice. Amino Acids Biotic Resour (氨基酸和生物资源), 2002, 24(3): 44-45 (in Chinese with English abstract)


[15]    Takahashi K. Abscisic acid as an endogenous stimulator for rice mesocotyl growth. Chem Regul Plants, 1985, 20(1): 53-60 (in Japanese with English abstract)
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