玉米杂交种先玉335及其亲本种子萌发过程中胚芽蛋白质组学分析

doi:10.3724/SP.J.1006.2011.01689

摘要/Abstract

摘要： 以培养84 h种子的胚芽(包括胚芽鞘)为试验材料, 对优良玉米杂交种先玉335与其亲本自交系的蛋白质组学差异进行了分析。结果表明, 在杂交种先玉335中共检测到560个蛋白点, 在亲本PH6WC和PH4CV分别检测到507个和508个蛋白点, 而且先玉335胚芽及胚芽鞘中81%的蛋白点表现非加性累积模式, 其中 288个蛋白点表现为超高亲的上调表达, 仅15个蛋白点表现超低亲的下调表达, 因此推测非加性蛋白的累积可能是杂交种先玉335胚芽萌发过程中胚芽及胚芽鞘杂种优势产生的主要原因。用于质谱分析的13个差异极显著蛋白主要涉及代谢途径、蛋白折叠、胁迫响应、细胞骨架和细胞解毒5种类型。

关键词: 玉米, 胚芽(胚芽鞘), 杂种优势, 蛋白质组学分析

Abstract: The plumule (including coleoptile) of seed cultured for 84 h was used as materials to analyze the proteomic difference between the elite hybrid Pioneer 335 and its parental lines by mean of 2-D electrophoresis method. The results showed that 560 protein spots were identified in Pioneer 335, 507 and 508 protein spots parental lines in its PH6WC and PH4CV, respectively. And in Pioneer 335, 81% protein spots exhibited nonadditive expression model. Out of them, 288 showed up-regulation with over high parent expression model. While only 15 showed down-regulation with over low parent expression model. Thus the accumulation of nonadditive expression protein might be the key factor resulting in heterosis at the tested development at stage in Pioneer 335 at least in this study. Notably, 13 significantly different protein spots between the hybrid and its parents were involved in metabolic pathway, protein folding, stress response, cytoskeleton and cell detoxification.

Key words: Maize, Plumule (including cleotiple), Heterosis, Proteomic analysis

进茜宁, 付志远, 丁冬, 刘宗华, 李卫华, 汤继华. 玉米杂交种先玉335及其亲本种子萌发过程中胚芽蛋白质组学分析[J]. 作物学报, 2011, 37(09): 1689-1694.

JIN Qian-Ning, FU Zhi-Yuan, DING Dong, LIU Zong-Hua, LI Wei-Hua, SHANG Ji-Hua. Proteomic Analysis of Plumule in Seed Germination for an Elite Hybrid of Pioneer 335 and Its Parental Lines in Maize[J]. Acta Agron Sin, 2011, 37(09): 1689-1694.

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