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作物学报 ›› 2012, Vol. 38 ›› Issue (09): 1665-1671.doi: 10.3724/SP.J.1006.2012.01665

• 耕作栽培·生理生化 • 上一篇    下一篇

水稻种子室温贮藏的适宜含水量及其生理基础

胡群文1,2，辛霞1，陈晓玲1，刘旭1,*，卢新雄1,*   

  1. 1 中国农业科学院作物科学研究所 / 农业部作物基因资源与种质创制重点实验室, 北京100081；2 安徽农业大学农学院, 安徽合肥230036
  • 收稿日期:2012-01-09 修回日期:2012-06-06 出版日期:2012-09-12 网络出版日期:2012-07-03
  • 通讯作者: 卢新雄, 刘旭, E-mail: xxlu@caas.net.cn, Tel: 010-62174099
  • 基金资助:

    本研究由中央级公益性科研院所基本科研业务费专项资助项目(201106)和农业部农作物种质资源保护利用项目(2130135)资助。

Optimal Moisture Content and Physiological Bases of Rice Seeds Storage at Room Temperature

HU Qun-Wen1,2,XIN Xia1,CHEN Xiao-Ling1,LIU Xu1,*,LU Xin-Xiong1,*   

  1. 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences /Key Laboratory of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Beijing 100081, China; 2 Anhui Agricultural University, Hefei 230036, China
  • Received:2012-01-09 Revised:2012-06-06 Published:2012-09-12 Published online:2012-07-03
  • Contact: 卢新雄, 刘旭, E-mail: xxlu@caas.net.cn, Tel: 010-62174099

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被引次数

9

摘要： 测定在南昌室温贮藏9年的含水量为10.6%、7.0%、6.0%、5.0%、4.0%和3.0%的水稻种子的种子生活力和活力、浸出物电导率, 以及胚中抗氧化酶活性、脂质过氧化产物丙二醛(MDA)含量, 以期了解水稻种子在室温贮藏条件下的适宜含水量及其生理基础。结果表明, 含水量为5.0%~6.0%的种子在室温贮藏9年后发芽率仍高于50%, 其他含水量种子的发芽率均显著低于50%, 可认为5.0%~6.0%是室温贮藏的适宜含水量。适宜含水量贮藏9年的种子吸胀12 h后, 胚中抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)活性显著高于其他含水量种子, 与在-18℃贮藏9年的种子(对照)相比, 活性相当或下降幅度最小；谷胱甘肽还原酶(GR)和超氧化物歧化酶(SOD)活性变化不显著。表明在适宜含水量下保持较高的APX和CAT活性, 可能是延缓种子生活力下降的重要因素之一。而种子浸出物电导率和胚中MDA含量等未表现出与种子生活力和含水量之间有显著相关性。此外含水量3.0%水稻种子的生活力和活力显著低于其他含水量水平的种子, 表明过度干燥会产生干燥损伤。

关键词: 水稻, 室温贮藏, 适宜含水量, 种子生活力, 抗氧化酶活性

Abstract: The objective of this study was to investigate the optimal moisture content for rice seeds storage and the physiological bases by measuring the seed viability, vigor index, malondialdehyde (MDA) content, leakage electrical conductivity, and antioxidant enzyme activities in the embryo of rice seeds, which had been stored at room temperature in Nanchang for nine years with moisture content (MC) at 10.6%, 7.0%, 6.0%, 5.0%, 4.0%, and 3.0% respectively. The data showed that after nine years storage at room temperature, the germination rates of seeds with MC of 5.0–6.0% were still higher than 50%, while that with other MCs much lower. Therefore the MC of 5.0–6.0% was regarded as the optimal moisture content (MCopt) for rice seed storage at room temperature. The activities of APX and CAT in the 12 h-imbied embryo of nine-year stored seeds with MCopt were much higher than those with other MCs, which showed no or minimal differences with those stored at -18℃. There were no significant changes in the activities of GR or SOD. These results suggested that the MCopt could maintain higher activities of APX and CAT, which might be one of the important reasons for retarding the reduction of seed viability during storage. No clear relationship was detected between seed leakage electrical conductivity or MDA content in embryo and seed viability or moisture content. When the seeds were dried to MC of 3.0%, the over-drying injury was introduced as the markedly decline in germination rate and germination index.

Key words: Rice (Oryza sativa L.), Storage at room temperature, Optimal moisture content, Seed viability, Antioxidase

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