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作物学报 ›› 2012, Vol. 38 ›› Issue (10): 1802-1809.doi: 10.3724/SP.J.1006.2012.01802

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘蓝型油菜不同发育时期株高QTL的动态分析

谢田田1,2，陈玉波3,2，黄吉祥2，张尧锋2，徐爱遐4，陈飞2，倪西源2，赵坚义2,*   

  1. 1浙江师范大学化学与生命科学学院，浙江金华321000；2浙江省农业科学院作物与核技术利用研究所，浙江杭州 310021；3杭州师范大学生命与环境科学学院，浙江杭州 310036；4 西北农林科技大学，陕西杨凌 712100
  • 收稿日期:2012-03-04 修回日期:2012-06-10 出版日期:2012-10-12 网络出版日期:2012-07-27
  • 通讯作者: 赵坚义, E-mail: jyzhao3@yahoo.com, Tel: 0571-86403406
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2011AA10A104), 浙江省科技厅重大专项(2011C12005)和浙江省基金重点项目(Z3100592)资助。

Dynamic Analysis of QTL for Plant Height of Rapeseed at Different Developmental Stages

XIE Tian-Tian1,2, CHEN Yu-Bo3,2, HUANG Ji-Xiang2, ZHANG Yao-Feng2, XU Ai-Xia 4, CHEN Fei2, NI Xi-Yuan2, and ZHAO Jian-Yi2,*   

  1. 1 College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321000, China; 2 Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 3 College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; 4 College of Agronomy, Northwest Agricultural and forestry University, Yangling 712100, China
  • Received:2012-03-04 Revised:2012-06-10 Published:2012-10-12 Published online:2012-07-27
  • Contact: 赵坚义, E-mail: jyzhao3@yahoo.com, Tel: 0571-86403406

PDF

1568

被引次数

11

摘要：

利用新版SG遗传图谱和282个SG-DH株系在中国西安、杭州和德国哥廷根3个生长环境下8个发育时期测定的株高数据，运用WinQTLCart2.5复合区间作图法以及结合条件遗传分析方法对其进行静态和动态QTL分析。结果显示, 来自品种Gaoyou等位基因在PHA3和PHC6两个QTL上同时存在时，可降低株高约20 cm；而当植株整合来自冬性品种Sollux的PHA9、PHC1和来自半冬性品种Gaoyou的PHA1、PHA3、PHC6时，株高可相应下降40 cm；环境对株高QTL的作用机理影响不大，但不同QTL的基因表达模式不同，存在来自双亲之一的等位基因控制株高和双亲等位基因在不同生长时期交替控制株高两种情况；通常株高QTL在中后期才能被检测到，但基因多在生长最为旺盛的短时期内表达，符合基因表达在先，性状表现在后的规律。解析株高性状在不同发育时期基因的累加效应和特定时段内的净表达效应，对克隆油菜株高基因和指导生产实践都将提供富有价值的科学信息和理论依据。

关键词: 油菜, 株高, 发育时期, 静态QTL, 动态QTL

Abstract:

The main objective of this study was to explore the genetic control mechanism of plant height in rapeseed and to reveal its dynamic gene expression in specific growing period. Taking advantage of the updated SG map, we determined phenotypic data of plant height from 282 SG-DH lines in three environments and eight development stages, and analyzed both static and dynamic quantitative trait locus (QTL) with unconditional and conditional mapping approach. The results showed that when alleles from Gaoyou existed in two major QTLs PHA3 and PHC6 synchronously, the plant height could decrease about 20 cm; when alleles of plant height came from Sollux in PHA9 and PHC1, and Gaoyou in PHA1, PHA3 and PHC6, the plantsfell 40 cm in height. The mechanism of QTL for plant height was less affected by environment, but various QTLs showed difference in gene expression patterns: plant height was dominated by alleles from one of parents or from two parents by turns at different stages. In general, the QTL for plant height could be detected from the middle to late stages, while the gene expression appeared shortly and only during the most active growing period, in congruent with the rule of “trait appearance goes after genetic expression”.

Key words: Brasscia napus L., Plant height, Developmental stage, Static QTL, Dynamic QTL

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