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作物学报 ›› 2012, Vol. 38 ›› Issue (12): 2206-2216.doi: 10.3724/SP.J.1006.2012.02206

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用与大豆灰斑病抗性基因连锁的SSR标记构建品种(系)的分子身份证

丁俊杰1,姜翠兰2,顾鑫1,杨晓贺1,赵海红1,申宏波1,仕相林4,刘春燕3,胡国华3,*,陈庆山4,*   

  1. 1黑龙江省农业科学院佳木斯分院 / 农业部佳木斯作物有害生物科学观测实验站,黑龙江佳木斯 154007;2 黑龙江农垦科学院作物研究所,黑龙江佳木斯 154007;3 黑龙江农垦科研育种中心,黑龙江哈尔滨 150030;4 东北农业大学农学院,黑龙江哈尔滨 150030
  • 收稿日期:2012-02-22 修回日期:2012-08-05 出版日期:2012-12-12 网络出版日期:2012-10-08
  • 通讯作者: 胡国华, E-mail: hugh757@vip.163.com; 陈庆山, E-mail: qshchen@sohu.com
  • 基金资助:

    本研究由黑龙江省自然科学基金项目(C200936), 国家公益性行业(农业)科研专项(201103016-03A2)和国家现代农业产业技术体系建设佳木斯综合试验站项目资助。

Establishment of Molecular ID of Soybean Varieties (Lines) with SSR Marker Linked to Resistance Genes of Cercospora sojina

DING Jun-Jie1,JIANG Cui-Lan2,GU Xin1,YANG Xiao-He1,ZHAO Hai-Hong1,SHEN Hong-Bo1,SHI Xiang-Lin3,LIU Chun-Yan3,HU Guo-Hua3,*,CHEN Qing-Shan4,*   

  1. 1 Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences / Observation and Experiment Station of Crop Pests of Jiamusi, Ministry of Agriculture, Jiamusi 154007, China; 2 Institute of Crop, Heilongjiang Academy of Land Reclamation Science, Jiamusi 154007, China; 3 Land Reclamation Research & Breeding Centre of Heilongjiang, Harbin 150090, China; 4 College of Agriculture, Northeast Agricultural University, Harbin 150030, China
  • Received:2012-02-22 Revised:2012-08-05 Published:2012-12-12 Published online:2012-10-08
  • Contact: 胡国华, E-mail: hugh757@vip.163.com; 陈庆山, E-mail: qshchen@sohu.com

摘要:

以黑龙江省29个大豆育种单位的103份已鉴定大豆灰斑病3个生理小种抗性的大豆品种(系)为材料,选择与大豆灰斑病抗病基因连锁的19个SSR标记检测,获得等位变异数86个,每个标记检测到的等位变异数分布在2~6个之间,平均为4.42个。应用遗传统计软件(genetics statistics 3.0)分析表明, 标记的多样性指数介于0.198~0.751之间,平均多样性指数为0.606。品种(系)特异指数差异较大,介于46.592~481.541之间,平均为87.415。根据标记的等位基因数,使用ID Analysis 1.0软件分析表明,利用与大豆抗灰斑病基因连锁的7个SSR标记(Satt565、Satt547、Satt431、Sct_186、SOYGPATR、Satt244、Sat_151)就能有效区分各品种(系),因此利用这7个标记构建了供试品种(系)的分子身份证。

关键词: 大豆灰斑病, 生理小种, SSR, 分子身份证

Abstract:

One hundred and three soybean varieties (lines) developed by 29 breeding units from six accumulated temperature zones in Heilongjiang were investigated. The resistances of 103 soybean varieties (lines) were identified with three races of Cercospora sonija. Eighty-six polymorphic alleles were detected with 19 SSR markers, for each primer, two to six allele variations were detected in all varieties (lines), with an average of 4.42. The diversity index of the 19 SSR loci ranged from 0.198 to 0.751 with an average of 0.606. The results of calculating specific index and genetic similarity among varieties (lines) showed that specific index of varieties (lines) ranged from 46.592 to 481.541, and the average was 87.415. According to fragment size of allele variation, the data calculated from the PAGE bands were analyzed by the software ID Analysis 1.0. Seven markers (Satt565, Satt547, Satt431, Sct_186, SOYGPATR, Satt244, and Sat_151) linked to the gene controlling resistance to Cercospora sojina were used to identify all 103 soybean lines, so that a set of molecular ID of soybean varieties (lines) was established.

Key words: Soybean frogeye leaf spot, Physiological race, SSR, Molecular ID

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