作物学报 ›› 2013, Vol. 39 ›› Issue (06): 1013-1020.doi: 10.3724/SP.J.1006.2013.01013
秦雁玲1,2,尹亮3,赵金凤2,孙伟3,赵庆雷3,袁守江3,朱文银3,郭宝太1,*,李学勇2,*
IN Yan-Ling1,2,YIN Liang3,ZHAO Jin-Feng2,SUN Wei3,ZHAO Qing-Lei3,YUAN Shou-Jiang3,ZHU Wen-Yin3,GUO Bao-Tai1,*,LI Xue-Yong2,*
摘要:
通过EMS诱变粳稻品种日本晴,筛选到2个矮秆突变体s1-46和s1-96。突变体主要表现为株高降低,分别为野生型的44.7%和55.9%,且叶片直立,穗粒数减少,籽粒变短。暗处理时,野生型的中胚轴伸长,但突变体的不伸长,说明突变性状与油菜素内酯(BR)相关。外源活性BR处理后,突变体与野生型的叶夹角均变大,根长均变短,表明突变基因与BR的生物合成相关。遗传分析表明,该突变性状由1对隐性基因控制。通过与籼稻品种Dular杂交构建F2群体,将该基因定位在第1染色体40.9 kb范围内。测序表明,该基因与参与BR生物合成的D2基因等位,其中s1-46第305位的氨基酸由脯氨酸突变成亮氨酸,而s1-96第370位的甘氨酸突变成谷氨酸。
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