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作物学报 ›› 2013, Vol. 39 ›› Issue (06): 1013-1020.doi: 10.3724/SP.J.1006.2013.01013

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

两个水稻D2基因等位突变体的表型分析及分子鉴定

秦雁玲1,2,尹亮3,赵金凤2,孙伟3,赵庆雷3,袁守江3,朱文银3,郭宝太1,*,李学勇2,*   

  1. 1 青岛农业大学生命科学学院,山东青岛266109;2 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程,北京100081;3 山东省水稻研究所,山东济南250100
  • 收稿日期:2012-11-13 修回日期:2013-01-18 出版日期:2013-06-12 网络出版日期:2013-03-22
  • 通讯作者: 郭宝太, E-mail: btguo12@163.com, Tel: 15969841765; 李学勇, E-mail: lixueyong@caas.cn, Tel: 010-82107409
  • 基金资助:

    本研究由国家重点基础研究发展计划(973计划)项目(2013CBA01401)和国家自然科学基金项目(31000696)资助。

Phenotypic Analysis and Molecular Characterization of Two Allelic Mutants of the D2 Gene in Rice

IN Yan-Ling1,2,YIN Liang3,ZHAO Jin-Feng2,SUN Wei3,ZHAO Qing-Lei3,YUAN Shou-Jiang3,ZHU Wen-Yin3,GUO Bao-Tai1,*,LI Xue-Yong2,*   

  1. 1 College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China; 2 National Key Facility for Crop Gene Resource and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Shandong Rice Research Institute, Jinan 250100, China
  • Received:2012-11-13 Revised:2013-01-18 Published:2013-06-12 Published online:2013-03-22
  • Contact: 郭宝太, E-mail: btguo12@163.com, Tel: 15969841765; 李学勇, E-mail: lixueyong@caas.cn, Tel: 010-82107409

摘要:

通过EMS诱变粳稻品种日本晴,筛选到2个矮秆突变体s1-46s1-96。突变体主要表现为株高降低,分别为野生型的44.7%55.9%,且叶片直立,穗粒数减少,籽粒变短。暗处理时,野生型的中胚轴伸长,但突变体的不伸长,说明突变性状与油菜素内酯(BR)相关。外源活性BR处理后,突变体与野生型的叶夹角均变大,根长均变短,表明突变基因与BR的生物合成相关。遗传分析表明,该突变性状由1对隐性基因控制。通过与籼稻品种Dular杂交构建F2群体,将该基因定位在第1染色体40.9 kb范围内。测序表明,该基因与参与BR生物合成的D2基因等位,其中s1-46305位的氨基酸由脯氨酸突变成亮氨酸,而s1-96370位的甘氨酸突变成谷氨酸。

关键词: 水稻, 矮秆突变体, 油菜素内酯, 精细定位

Abstract:

Two dwarf mutants s1-46 and s1-96 were isolated from an EMS mutated japonica cultivar Nipponbare. The plant height of the two mutants was 44.7% and 55.9% of that of the wild-type plant, respectively. The mutants also exhibited erect leaves, reduced grain number,,and shortened grains. When grown in complete darkness, the mesocotyl elongated in the wild-type plant, but not in the mutants, indicating that s1-46 and s1-96 are brassinosteroids (BRs)-related mutants. Furthermore, treatment of seedling with bioactive BR showed that the leaf angle was increased and the root length was decreased in both the wild-type and mutant plants, suggesting that the mutated gene is involved in BR biosynthesis. Genetic analysis showed that the phenotypes of both mutants were caused by a recessive gene. Fine-mapping showed that this gene was located within a 40.9 kb region on chromosome 1. Sequence analysis revealed that the mutated gene was allelic to D2, encoding CYP90D2that is involved in BR biosynthesis. In s1-46 and s1-96, the encoded amino acids were changed from proline to leucine and from glycine to glutamic acid at the 305th and370thpositions of D2, respectively.

Key words: Rice, Dwarf mutant, BR, Fine-mapping

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