作物学报 ›› 2013, Vol. 39 ›› Issue (11): 1927-1934.doi: 10.3724/SP.J.1006.2013.01927
于苗苗1,2,3,戴正元1,潘存红1,陈夕军2,余玲1,张晓祥1,李育红1,肖宁1,龚红兵3,盛生兰3,潘学彪2,张洪熙1,李爱宏1,*
YU Miao-Miao1,2,3,DAI Zheng-Yuan1,PAN Cun-Hong1,CHEN Xi-Jun2,YU Ling1,ZHANG Xiao-Xiang1,LI Yu-Hong1,XIAO Ning1,GONG Hong-Bing3,SHENG Sheng-Lan3,PAN Xue-Biao2,ZHANG Hong-Xi1,LI Ai-Hong1,*
摘要:
Pigm是Pi2基因簇的等位或紧密连锁基因。本研究构建了4个不同遗传背景下Pigm和Pi2的系列近等基因系,204个菌株苗期接种结果显示其抗性频率均超过70%,但Pigm和Pi2的抗谱重叠度仅为54.4%~65.7%,聚合Pi1/Pigm和Pi1/Pi2杂种的抗性频率均超过90%。穗瘟人工接种及病圃自然诱发鉴定表现与苗期接种一致的发病趋势。农艺性状调查结果显示获得的近等基因系与其轮回亲本基本相似,存在较少的累赘连锁。表明Pigm是一个与Pi2抗谱差异明显的广谱抗性基因,对稻瘟病抗性育种具有重要应用价值。
[1] Liu G, Lu G, Zeng L, Wang G L. Two broad-spectrum blast resistance genes Pi9(t) and Pi2(t), are physically linked on rice chromosome 6. Mol Genet Genomics, 2002, 267: 472–480[2] Ahn S N, Kim Y K, Hong H C, Han S S, Kwon S J, Choi H C, Moon H P, McCouch S R. Molecular mapping of a new gene for resistance to rice blast (Pyricularia grisea Sacc.). Euphytica, 2000, 116: 17–22[3] Wu J L, Fan Y Y, Li D B, Zheng K L, Leung H, Zhuang J Y. Genetic control of rice blast resistance in the durably resistant cultivar Gumei 2 against multiple isolates. Theor Appl Genet, 2005, 111: 50–56[4] Jeung J U, Kim B R, Cho Y C, Han S S, Moon H P, Lee Y T, Jena K K. A novel gene, Pi40(t), linked to the DNA markers derived from NBS-LRR motifs confers broad spectrum of blast resistance in rice. Theor Appl Genet, 2007, 115: 1163–1177[5] Zhu X Y, Chen S, Yang J Y, Zhou S C, Zeng L X, Han J L, Su J, Wang L, Pan Q H. The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family. Theor Appl Genet, 2012, 124: 1295–1304[6] Hayashi K, Yoshida H, Ashikawa I. Development of PCR-based allele-specific and InDel marker sets for nine rice blast genes. Theor Appl Genet, 2006, 113: 251–260[7] Qu S, Liu G, Zhou B, Bellizzi M, Zeng L, Dai L, Han B, Wang G L. The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice. Genetics, 2006, 172: 1901–1914[8] Deng Y W, Zhu X D, Shen Y, He Z H. Genetic characterization and fine mapping of the blast resistance locus Pigm(t) tightly linked to Pi2 and Pi9 in a broad-spectrum resistant Chinese variety. Theor Appl Genet, 2006, 113(4): 705–713[9] Chen Z-W(陈志伟), Guan H-Z(官华忠), Wu W-R(吴为人), Zhou Y-C(周元昌), Han Q-D(韩庆典). The screening of molecular markers closely linked to rice blast resistant gene Pi-1 and their application. J Fujian Agric For Univ (Nat Sci Edn) (福建农林大学学报•自然科学版), 2005, 34(1): 74–77 (in Chinese with English abstract)[10] Hua L X, Wu J Z, Chen C X, Wu W H, He X Y, Lin F, Wang L, Ashikawa I, Matsumoto T, Wang L, Pan Q H. The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast. Theor Appl Genet, 2012, 125: 1047–1055[11] Chen H-Q(陈红旗), Chen Z-X(陈宗祥), Ni S(倪深), Zuo S-M(左示敏), Pan X-B(潘学彪), Zhu X-D(朱旭东). Pyramiding three genes with resistance to blast by marker-assisted selection to improve rice blast resistance of Jin 23B. Chin J Rice Sci (中国水稻科学), 2008, 22(1): 23–27 (in Chinese with English abstract)[12] Liu W-G(柳武革), Wang F(王丰), Jin S-J(金素娟), Zhu X-Y(朱小源), Li J-H(李金华), Liu Z-R(刘振荣), Liao Y-L(廖亦龙), Zhu M-S(朱满山), Huang H-J(黄慧君), Fu F-H(符福鸿), Liu Y-B(刘宜柏). Improvement of rice blast resistance in TGMS line by pyramiding of Pi-1 and Pi-2 through molecular marker-assisted selection. Acta Agron Sin (作物学报), 2008, 34(7): 1128–1136 (in Chinese with English abstract)[13] Jiang H C, Feng Y T, Bao L, Li X, Gao G J, Zhang Q L, Xiao J H, Xu C G, He Y Q. Improving blast resistance of Jin 23B and its hybrid rice by marker-assisted gene pyramiding. 2012, 30: 1679–1688[14] Lu Y-J(卢扬江), Zheng K-L(郑康乐). A simple method for extracting rice DNA. Chin J Rice Sci (中国水稻科学), 1992, 6(1): 47–48 (in Chinese with English abstract)[15] Hospital F, Chevalet C, Mulsant P. Using markers in gene introgression breeding programs. Genetics, 1992, 132: 1199–1210[16] Zhou Y-J(周益军), Fan Y-J(范永坚), Wu S-H(吴淑华), Lu Z-X(陆振晓), Cheng Z-B(程兆榜). Identification techniques of rice blast fungus races by in vitro inoculation and artificial mutation of their phthogenicity. Jiangsu Agric Res (江苏农业研究), 1999, 20(1): 34–38 (in Chinese with English abstract)[17] Luo C-P(罗楚平), Ni L(倪磊), Chen Z-Y(陈志谊), Liu Y-F(刘永锋), Liu Y-Z(刘邮洲), Nie Y-F(聂亚锋). Inoculation and identification techniques for rice blast and resistance screening of cultivars from Jiangsu rice regional test in 2009. Jiangsu Agric Sci (江苏农业科学), 2009, (6): 178–179 (in Chinese with English abstract)[18] Zhang L(张磊), Zhang Q-J(张启军), Cheng Z-B(程兆榜), Yang H(杨豪), Zhou Y-J(周益军), Lü C-G(吕川根). Resistances of four sets of rice varieties to 19 strains of magnaporthe griseas in Jiangsu area. Jiangsu J Agric Sci (江苏农业学报), 2010, 26(5): 948–953 (in Chinese with English abstract)[19] Chen F-R(陈福如), Ruan H-C(阮宏椿), Yang X-J(杨秀娟), Lin S-C(林时迟), Fang Q(方琴), Yan Y(严琰). The correlation in seeding blast, leaf blasts and neck blasts of rice. Chin Agric Sci Bull (中国农学通报), 2006, 22(7): 440–443 (in Chinese with English abstract) [20] Zhang X-T(张学堂), Liao X-H(廖新华), Zhu Z-H(朱振华), Su Z-X(苏振喜), Shi R(世荣), Zhao G-Z(赵国珍), Jiang C(蒋聪), Dai L-Y(戴陆园). Applications of Pi-z, Pi-ta2 genes in Japonica rice breeding with blast resistane in Yunnan. J Agric Sci Technol (中国农业科技导报), 2010, 12(1): 100–105 (in Chinese with English abstract)[21] Islam M R, Shepherd K W. Present status of genetics of rust resistance in flax. Euphytica, 1991, 55: 255–267[22] Ellis J G, Lawrence G J, Finnegan E J, Anderson P A. Contrasting complexity of two rust resistance loci in flax. Proc Natl Acad Sci USA, 1995, 92: 4185–4188 [23]Yang Z, Sun X, Wang S P, Zhang Q F. Genetic and physical mapping of a new gene for bacterial blight resistance in rice. Theor Appl Genet, 2003, 106: 1467–1472[24] Zhou B, Qu S, Liu G, Dolan M, Sakai H, Lu G, Bellizzi M, Wang G L. The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea. Mol Plant-Microbe Interact, 2006, 19: 1216–1228[25] Dai L, Wu J, Li X, Wang X, Liu X, Jantasuriyarat C, Kudrna D, Yu Y, Wing R A, Han B. Genomic structure and evolution of the Pi2/9 locus in wild rice species. Theor Appl Genet, 2010, 121: 295–309[26] Yang J-Y(杨健源), Chen S(陈深), Zeng L-X(曾列先), Li Y-L(李亦龙), Chen Z(陈珍), Zhu X-Y(朱小源). Evaluation on resistance of major rice blast resistance genes to agnaporthe grisea isolates collected from indica rice in Guangdong Province. Chin J Rice Sci (中国水稻科学), 2008, 22(2): 190–196 (in Chinese with English abstract)[27] Yano M, Katayose Y, Ashikari M, Yamanouchi U, Monna L, Fuse T, Baba T, Yamanoto K, Umehara Y, Nagamura Y, Sasaki T. Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell, 2000, 12: 2473–2483 |
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