作物学报 ›› 2013, Vol. 39 ›› Issue (11): 2065-2073.doi: 10.3724/SP.J.1006.2013.02065
周卫霞1,王秀萍2,穆心愿1,李潮海1,*
ZHOU Wei-Xia1,WANG Xiu-Ping2,MU Xin-Yuan1,LI Chao-Hai1,*
摘要:
以不耐阴型玉米豫玉22和耐阴型玉米郑单958为试验材料,设置自然光照和弱光胁迫两个处理,研究弱光胁迫对不同基因型玉米雌雄花发育的影响。研究结果表明,弱光胁迫延缓了雌雄穗的生长发育,表现为抽雄、吐丝和盛花期延迟,花期和雌雄间隔期延长。弱光胁迫下,在吐丝期不耐阴型玉米花粉量比对照显著增加,耐阴型玉米花粉量与对照差异较小;不耐阴型玉米花粉表面网纹在弱光胁迫下变粗且间隙增大,花粉萌发孔及其附近严重畸形,有的明显内陷,花粉内淀粉粒数目显著减少,营养供应能力减弱,耐阴型玉米在弱光胁迫下花粉表面网纹略有加粗或没有变化,萌发孔略微凹陷且程度远低于不耐阴型玉米,花粉中淀粉粒密度略有降低。遮光处理后,不耐阴型玉米的花粉活力、花粉萌发率和萌发速率表现为下降,而耐阴型玉米表现为上升。花丝的生长速率及伸出苞叶数量在弱光胁迫下显著下降,果穗上部花丝受到的影响最大,向下逐渐减弱。弱光胁迫下两个基因型玉米穗长和穗粒数减少,不耐阴型玉米受弱光胁迫影响的程度高于耐阴型玉米。雌雄间隔期延长、营养供应能力减弱导致的花粉畸形、花丝生长速率和可授粉花丝数目的减少以及籽粒IAA含量的下降和ABA含量的增加是弱光条件下玉米穗粒数显著减少的主要原因。
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