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作物学报 ›› 2014, Vol. 40 ›› Issue (08): 1470-1484.doi: 10.3724/SP.J.1006.2014.01470

• 耕作栽培·生理生化 • 上一篇    下一篇

低温胁迫对微胚乳超甜超高油玉米耐寒性生理生化特性的影响

郝小琴,姚鹏鹤,高峥荣,吴子恺   

  1. 广西大学农学院，广西南宁 530005
  • 收稿日期:2013-10-18 修回日期:2014-04-16 出版日期:2014-08-12 网络出版日期:2014-06-03
  • 基金资助:

    本研究由广西自然科学基金项目(2012GXNSFAA053048)和南宁市科学研究与技术开发计划项目(20122064)资助。

Effects of Low Temperature Stress on the Physiological and Biochemical Characteristics of Cold Tolerance in Micro-endosperm Super Sweet and Super High Oil Maize

HAO Xiao-Qin,YAO Peng-He,GAO Zheng-Rong,WU Zi-Kai   

  1. Agricultural College of Guangxi University, Nanning 530005, China
  • Received:2013-10-18 Revised:2014-04-16 Published:2014-08-12 Published online:2014-06-03

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摘要：

为了解新型种质微胚乳超甜超高油玉米(简称为微胚乳玉米)的耐寒性，选用5个微胚乳玉米杂交组合(ME)，以高油115和正甜68为对照，在发芽期和苗期低温胁迫下，测定发芽率、发芽势、相对电导率、丙二醛含量、脯氨酸含量、可溶性糖含量、可溶性蛋白含量、叶绿素含量以及SOD、POD活性等生理生化指标，鉴定其耐寒性，并利用模糊隶属函数法综合评定其耐寒性。结果表明，ME1、ME2、ME3和高油115在发芽期的相对电导率上升幅度较小，脯氨酸含量和SOD活性上升幅度较大；而ME5、ME4和正甜68在发芽期的相对电导率上升幅度较大，脯氨酸含量和SOD活性上升幅度较小。利用相对电导率、脯氨酸含量、SOD活性3个指标能较有效预测微胚乳玉米在发芽期的耐寒性。ME2、ME1和高油115在苗期的丙二醛含量上升幅度较小，脯氨酸含量、SOD和POD活性上升幅度较大；而ME5和正甜68在苗期的丙二醛含量上升幅度较大，脯氨酸含量、SOD和POD活性上升幅度较小。利用丙二醛含量、脯氨酸含量、SOD和POD活性4个指标能较好地预测微胚乳玉米在苗期的耐寒性。以隶属函数值综合评价表明，5个微胚乳玉米材料的耐寒性均强于正甜68。ME2在发芽期和苗期都表现较强的耐寒性，其次为ME1和 ME3，而ME4和ME5耐寒性均较弱。

关键词: 微胚乳超甜超高油玉米, 低温胁迫, 耐寒性, 生理生化特性

Abstract:

In the germination and seedling stages of the maize, five micro-endosperm super sweet and super high oil maize (abbreviation as ME) hybrid combinations, two control materials Gaoyou 115 and Zhengtian 68 were used to study the changes of physiological and biochemical characteristics, such as germination rate, germination energy, relative conductivity, contents of MDA, proline, soluble sugar, soluble protein, chlorophyll, and SOD, POD activities under low temperature stress. At the same time, the identification of cold tolerance of the materials were conducted. Based on the multiple physiological and biochemical parameters, we employed the fuzzy membership function to evaluate the cold tolerance of the experiment materials comprehensively. The results showed that the relative conductivity had a little increase, but the proline content and SOD activity increased largely in ME1, ME2, ME3, and Gaoyou 115, as compared with ME5, ME4 and Zhengtian68 in the germination stage. It was advised that relative conductivity, proline content and SOD activity could be used to predict cold tolerance of the materials more effectively in germination stage. In the seedling stage, compared with ME5 and Zhengtian 68, the MDA content increased a little and activities of SOD, POD and proline content increased largely in ME2, ME1, and Gaoyou 115. So we thought that MDA content, proline content, SOD and POD activities can be used to predict the cold tolerance of the experiment materials in seedling stage. Meanwhile, membership function value showed that the cold tolerance of the five ME materials was stronger than that of Zhengtian 68. Among the five ME materials, ME2 showed a strong ability to tolerate low temperature stress in germination and seedling stages, ME1 and ME3 showed moderate tolerance, and ME4 and ME5 showed weak tolerance.

Key words: Micro-endosperm super sweet and super high oil maize, Low temperature stress, Cold tolerance, Physiological and biochemical characteristics

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