作物学报 ›› 2014, Vol. 40 ›› Issue (10): 1725-1732.doi: 10.3724/SP.J.1006.2014.01725
朱玉磊,王升星,赵良侠,张德新,胡建帮,曹雪连,杨亚杰,常成,马传喜,张海萍*
ZHU Yu-Lei,WANG Sheng-Xing,ZHAO Liang-Xia,ZHANG De-Xin,HU Jian-Bang,CAO Xue-Lian,YANG Ya-Jie,CHANG Cheng,MA Chuan-Xi,ZHANG Hai-Ping*
摘要:
利用分布于小麦全基因组的181对分子标记,分析264份自然群体的基因型,采用TASSLE软件的GLM和MLM模型检测与整穗发芽抗性紧密关联的标记位点,发掘相关位点内的优异等位变异。在2012年和2013年室内整穗发芽率、2013年田间自然降雨整穗发芽率3个环境中,共关联到20个显著位点(P<0.05),分布于小麦染色体1AS、2DS、3AS、3BL、4AL、5AS、5BL、6BS、6DS、7AL和7BL上。分别位于2DS和7BL上的分子标记gwm102和barc340同时在3个环境下关联到,属于稳定的抗性位点; 另有6个标记位点同时在2个环境下关联到; 其余12个标记位点仅在1个环境下关联到。位于7BL上的barc340标记位点为一新报道位点。从重复关联的8个标记位点内共检测出10种优异等位变异。barc28-229bp和barc28-217bp对提高整穗发芽抗性效应最显著,主要分布在地方品种中(如遂宁坨坨麦等),而gwm102-142bp和barc186-199bp效应虽然相对较小,但多分布在推广品种中(如扬麦158等),有利于穗发芽抗性分子育种的直接应用。
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