不同水氮处理对豫麦49-198籽粒抗氧化物含量的影响

doi:10.3724/SP.J.1006.2014.02046

摘要/Abstract

摘要：

2012-2013年度, 在温县和郑州大田条件下, 研究不同水氮处理对冬小麦品种豫麦49-198籽粒总酚、类黄酮、类胡萝卜素含量及抗氧化活性的影响。结果表明, 在施纯氮0~300 kg hm^–2范围内, 所有观测指标均随施氮量的增加而增加, 以施氮300 kg hm^–2处理最高。随灌水次数(0~2次)的增加, 总酚、类黄酮含量和抗氧化活性呈先增加后降低趋势, 以灌拔节水处理最高；类胡萝卜素含量在不同试点间表现不一致。水氮耦合, 以灌拔节水+施氮240~300 kg hm^–2处理的抗氧化物含量及抗氧化活性较高, 而总酚、类黄酮及类胡萝卜素的积累量则以灌拔节和开花水+施氮240~300 kg hm^–2处理较高。相关分析表明, 籽粒总酚、类黄酮含量与抗氧化活性均呈显著正相关, 表明总酚、类黄酮含量增加可以提高小麦籽粒抗氧化活性；不同深度土层土壤水分含量及硝态氮含量与籽粒抗氧化物质含量的相关性存在差异, 总体而言, 氮含量有助于总酚及类胡萝卜素含量的积累, 而水分含量可能有助于类黄酮含量的提高。

关键词: 灌水, 氮肥, 冬小麦, 抗氧化物质, 籽粒

Abstract:

Two field experiments were carried out in Wenxian and Zhengzhou of Henan Province in 2012–2013 wheat season to study the effects of nitrogen (N) application rate and irrigation on total phenol, flavonoids, and carotenoid contents and antioxidant activity in winter wheat cultivar Yumai 49-198. In the N application range of 0–300 kg ha^-1, all indices tested increased with increasing N rate and reached peak values under 300 kg ha^-1. With the increase of irrigation frequency (0–2 irrigations), total phenol and flavonoids contents and antioxidant activity had the maximum values under one irrigation condition at jointing, whereas the carotenoid content varied across locations. The interaction between N application rate and irrigation had a significant effect on antioxidants in wheat grain. Treatments of irrigation at jointing stage plus N application of 240–300 kg ha^–1 resulted in the high antioxidant content and antioxidant activity, whereas twice irrigation at jointing and anthesis stages plus N application of 240–300 kg ha^–1 resulted in antioxidant accumulation. Total phenol and flavonoids contents were positively correlated with antioxidant activity, indicating that high contents of total phenol and flavonoids were favorable for high antioxidation. The correlations of grain antioxidant content with soil moisture and NO₃^--N content varied in different soil layers. Generally, N fertilizer had the effect on increasing total phenol and carotenoid contents and soil water had the effect on accumulating flavonoids.

Key words: Irrigation, Nitrogen fertilization, Winter wheat, Antioxidant, Grain

孙德祥,马冬云*,王晨阳,李耀光,刘卫星,李秋霞,冯伟,郭天财*. 不同水氮处理对豫麦49-198籽粒抗氧化物含量的影响[J]. 作物学报, 2014, 40(11): 2046-2051.

SUN De-Xiang,MA Dong-Yun*,WANG Chen-Yang,LI Yao-Guang,LIU Wei-Xing,LI Qiu-Xia,FENG Wei,GUO Tian-Cai*. Effects of Irrigation and Nitrogen on Antioxidant Contents in Yumai 49-198 Grains[J]. Acta Agron Sin, 2014, 40(11): 2046-2051.

参考文献

[1]石碧, 狄莹. 植物多酚. 北京: 科学出版社, 2000

Shi B, Di Y. Plant Polyphenol. Beijing: Science Press, 2000 (in Chinese)

[2]Onyeneho S N, Hettiarachchi N S. Antioxidant activity of durum wheat bran. J Agric Food Sci, 1992, 40: 1496–1500

[3]Liu S, Stampfer H J, Giovannucci E, Rimm E, Manson J E, Hennekens C H, Willett W C. Whole grain consumption and risk of coronary heart disease: Result from the nurses’ health study. Am Clin Nutr, 2000, 71: 412–419

[4]Liu S, Stampfer H J, Rexrode, K M, Hu F B, Willett W C. Whole grain consumption and risk of ischemic stroke in women: a prospective study. Am Med Assoc, 2000, 90: 1534–1540

[5]Liu S, Stampfer H J, Rexrode, K M, Hu F B, Giovannucci E, Colditz G A, Manson Je, Hennekens C H, Willett W C. A prospective study of whole grain intake and risk of type 2 diabetes mellitus in US women. Am Public Health, 2000, 90: 1409–1415

[6]Marquart L, Jacobs D R, Slavin J L. Whole grains and health: An overview. Am Coll Nutr, 2000, 19: 289S–301S

[7]Fester T, Schmidt D, Lohse S, Walter M H, Giuliano G, Bramley P M, Fraser P D, Hause B, Strack D. Stimulation of carotenoid metabolism in arbuscular mycorrhizal roots. Planta, 2002, 216: 148–154

[8]宗学凤, 张建奎, 李帮秀, 余国东, 石有明, 王三根. 小麦籽粒颜色与抗氧化作用. 作物学报, 2006, 62: 238–241

Zong X F, Zhang J K, Li B X, Yu G D, Shi Y M, Wang S G. Relationship between antioxidation and grain colors of wheat (Ttiticum aestivum L.). Acta Agron Sin, 2006, 32: 238–241 (in Chinese with English abstract)

[9]Engert N, John A, Henning W, Honermeier B. Effect of sprouting on the concentration of phenolic acids and antioxidative capacity in wheat cultivars (Triticum aestivum ssp. aestivum L.) in dependency of nitrogen fertilization. J Appl Bot Food Q, 2011, 84: 111–118

[10]Alizdeh A, Khoshkhui M, Javidnia K, Firuzi O, Tafazoli E, Khalighi. Effect of fertilizer on yield, essential oil composition, total phenolic content and antioxidant activity in Satureja hortensis L. (Lamiaceae) cultivated in Iran. J Med Plant Res, 2010, 4: 33–39

[11]Konopka I, Tanska M, Faron A, Stepien A, Wojtkowiak K. Comparison of the phenolic compounds, carotenoids and tocochromanols content in wheat grain under organic and mineral fertilization regimes. Molecules, 2012, 17: 12341–12355

[12]梁宗锁, 杨东风, 杨宗岐, 韩蕊莲, 刘晓蕾. 不同土壤水分对丹参叶片总酚酸类成分积累及相关酶活性的影响. 浙江理工大学学报, 2013, 30: 573–578

Liang Z S, Yang D F, Yang Z Q, Han R L, Liu X L. Effect of different soil moistures on total phenolic acid composition accumulation and relevant enzymatic activity of Salvia miltiorrhiza Lamina. J Zhejiang Sci-Tech Univ, 2013, 30: 573–578 (in Chinese with English abstract)

[13]王爱华, 王松峰, 宫长荣. 氮素用量对烤烟上部叶片多酚类物质动态的影响. 西北农林科技大学学报(自然科学版), 2005, 33(3): 57–60

Wang A H, Wang S F, Gong C R. The dynamic effects of nitrogen rate on polyphenols of flue-cured tobacco upper leaf. J Northwest Sci Tech Univ Agric For (Nat Sci Edn), 2005, 33(3): 57–60 (in Chinese with English abstract)

[14]American Association of Cereal Chemist: Approved Methods of the AACC, 9th Edn. The Association: St. Paul, MN, U.S.A. 1995

[15]Shen Y, Jin L, Xiao P, Lu Y, Bao J S. Total phenolics, ?avonoids, antioxidant capacity in rice grain and their relations to grain color, size and weight. J Cereal Sci, 2009, 49: 106–111

[16]Bao J S, Cai Y, Sun M, Wang G Y, Corke H. Anthocyanins, ?avonols, and free radical scavenging activity of Chinese bayberry (Myrica rubra) extracts and their color properties and stability. J Agric Food Chem, 2005, 53: 2327–2332

[17]Miller N J, Diplock A T, Rice E C, Davies M J, Gopinathan V, Milner A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci, 1993, 84: 407–412

[18]Mogren L M, Olsson M E, Gertsson U E. Quercetin content in field-cured onions (Allium cepa L.): effects of cultivar, lifting time, and nitrogen fertilizer level. J Agric Food Chem, 2006, 54: 6185–6191

[19]Nguyen P M, Niemeyer E D. Effects of nitrogen fertilization on the phenolic composition and antioxidant properties of Basil (Ocimum basilicum L.). J Agric Food Chem, 2008, 56: 8685–8691

[20]王祥军, 齐军仓, 贾力群, 王倩, 王琴, 马建峰, 王仙, 曹连莆. 氮素对大麦籽粒中酚酸及蛋白质含量的影响. 核农学报, 2011, 25: 162–168

Wang X J, Qi J C, Jia L Q, Wang Q, Wang Q, Ma J F, Wang X, Cao L P. Effect of nitrogen on phenolic acid and protein content in barley (Hordeum vulgare L.) grain. J Nucl Agric Sci, 2011, 25: 162–168 (in Chinese with English abstract)

[21]Margna U. Control at the level of substrate supply: an alternative in the regulation of phenylpropanoid accumulation in plant cells. Phytochemistry, 1977, 16: 419–426

[22]Shih C H, Chu H, Tang L K, Sakamoto W, Maekawa M, Chu I K, Wang M F, Lo C. Functional characterization of key structural genes in rice flavonoid biosynthesis. Planta, 2008, 228: 1043–1054

[23]Herms D A, Mattson W J. The dilemma of plants: to grow or defend. Q Rev Biol, 1992, 67: 283–335

[24]Bryant J P, Chapin III F S, Klein D R. Carbon/nutrient balance of boreal plants in relation to vertebrate herbivory. J Ecol, 1983, 40: 357–368

[25]冯虎元, 安黎哲, 陈书燕, 王勋陵, 程国栋. 增强UV-B辐射与干旱复合处理对小麦幼苗生理特性的影响. 生态学报, 2002, 22: 1564–1567

Feng H Y, An L Z, Chen S Y, Wang X L, Cheng G D. The interactive effect of enhanced UV-B irradiation and water deficit on physiological properties of spring wheat seeding. Acta Ecol Sin, 2002, 22: 1564–1567 (in Chinese with English abstract)

[26]陈义强, 刘国顺, 凌爱芬, 张毅, 王芳, 习红昂. 氮、磷、钾肥和土壤水分对烟草烤后叶中类胡萝卜素含量的影响. 植物生理学通讯, 2008, 44: 279–282

Chen Y Q, Liu G S, Ling A F, Zhang Y, Wang F, Xi H A. Effects of nitrogen, phosphate, potassium fertilizers and soil water on cartenoids contents of flue-cured tobacco (Nicotiana tabacum L.) leaves. Plant Physiol Commun, 2008, 44: 279–282 (in Chinese with English abstract)

[27]Oliveira C, Silva Ferreira A C, Mendes Pinto M, Hogg T, Alves F, Guedes D E, Pinho P. Carotenoid compounds in grapes and their relationship to plant water status. J Agric Food Chem, 2003, 51: 5967–5971

[28]He X Y, He Z H, Ma W, Appels R, Xia X C. Allelic variants of phytoene synthase1 (Psy1) genes in Chinese and CIMMYT wheat cultivars and development of functional markers for ?our colour. Mol Breed, 2009, 23: 553–563

相关文章 15

[1]	郑小龙, 周菁清, 白杨, 邵雅芳, 章林平, 胡培松, 魏祥进. 粳稻不同穗部籽粒的淀粉与垩白品质差异及分子机制[J]. 作物学报, 2022, 48(6): 1425-1436.
[2]	陈静, 任佰朝, 赵斌, 刘鹏, 张吉旺. 叶面喷施甜菜碱对不同播期夏玉米产量形成及抗氧化能力的调控[J]. 作物学报, 2022, 48(6): 1502-1515.
[3]	徐田军, 张勇, 赵久然, 王荣焕, 吕天放, 刘月娥, 蔡万涛, 刘宏伟, 陈传永, 王元东. 宜机收籽粒玉米品种冠层结构、光合及灌浆脱水特性[J]. 作物学报, 2022, 48(6): 1526-1536.
[4]	郭星宇, 刘朋召, 王瑞, 王小利, 李军. 旱地冬小麦产量、氮肥利用率及土壤氮素平衡对降水年型与施氮量的响应[J]. 作物学报, 2022, 48(5): 1262-1272.
[5]	闫宇婷, 宋秋来, 闫超, 刘爽, 张宇辉, 田静芬, 邓钰璇, 马春梅. 连作秸秆还田下玉米氮素积累与氮肥替代效应研究[J]. 作物学报, 2022, 48(4): 962-974.
[6]	徐宁坤, 李冰, 陈晓艳, 魏亚康, 刘子龙, 薛永康, 陈洪宇, 王桂凤. 一个新的玉米Bt2基因突变体的遗传分析和分子鉴定[J]. 作物学报, 2022, 48(3): 572-579.
[7]	袁嘉琦, 刘艳阳, 许轲, 李国辉, 陈天晔, 周虎毅, 郭保卫, 霍中洋, 戴其根, 张洪程. 氮密处理提高迟播栽粳稻资源利用和产量[J]. 作物学报, 2022, 48(3): 667-681.
[8]	冯健超, 许倍铭, 江薛丽, 胡海洲, 马英, 王晨阳, 王永华, 马冬云. 小麦籽粒不同层次酚类物质与抗氧化活性差异及氮肥调控效应[J]. 作物学报, 2022, 48(3): 704-715.
[9]	杨谨, 白爱宁, 白雪, 陈娟, 郭林, 刘春明. 水稻胚胎和胚乳双缺陷突变体eed1的表型与遗传分析[J]. 作物学报, 2022, 48(2): 292-303.
[10]	渠建洲, 冯文豪, 张兴华, 徐淑兔, 薛吉全. 基于全基因组关联分析解析玉米籽粒大小的遗传结构[J]. 作物学报, 2022, 48(2): 304-319.
[11]	张特, 王蜜蜂, 赵强. 滴施缩节胺与氮肥对棉花生长发育及产量的影响[J]. 作物学报, 2022, 48(2): 396-409.
[12]	张军, 周冬冬, 许轲, 李必忠, 刘忠红, 周年兵, 方书亮, 张永进, 汤洁, 安礼政. 淮北地区麦茬机插优质食味粳稻氮肥减量的精确运筹[J]. 作物学报, 2022, 48(2): 410-422.
[13]	闫岩, 张钰石, 刘础荣, 任丹阳, 刘洪润, 刘雪晴, 张明才, 李召虎. 冬小麦-夏玉米轮作“双晚”种植模式下的品种匹配与资源效率[J]. 作物学报, 2022, 48(2): 423-436.
[14]	王洋洋, 贺利, 任德超, 段剑钊, 胡新, 刘万代, 郭天财, 王永华, 冯伟. 基于主成分-聚类分析的不同水分冬小麦晚霜冻害评价[J]. 作物学报, 2022, 48(2): 448-462.
[15]	张倩, 韩本高, 张博, 盛开, 李岚涛, 王宜伦. 控失尿素减施及不同配比对夏玉米产量及氮肥效率的影响[J]. 作物学报, 2022, 48(1): 180-192.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed