作物学报 ›› 2015, Vol. 41 ›› Issue (02): 251-258.doi: 10.3724/SP.J.1006.2015.00251
张勇,张晓,郭杰,高德荣,张伯桥*
ZHANG Yong,ZHANG Xiao,GUO Jie,GAO De-Rong,ZHANG Bo-Qiao*
摘要:
溶剂保持力(SRC)是软质小麦鉴定评价的重要指标。为获得与SRC关联的分子标记,提高育种效率,对不同硬度类型的176份品种的乳酸SRC、碳酸钠SRC、蔗糖SRC和水SRC进行SSR标记检测,并结合其在江苏里下河地区连续3个生长季的4种SRC表型数据,利用MLM模型进行了关联分析。以236对SSR引物共检测出1340个等变位变异,平均每个位点5.5个等位变异,平均PIC值为0.4663。共检测到28个关联位点(P < 0.005),单个位点可解释3.19%~21.84%的表型变异;与乳酸SRC、水SRC、蔗糖SRC和碳酸钠SRC相关联的位点分别为13、7、6和2个;与水SRC关联的gwm642-1D在3年中均被检测到。在这些关联位点上发现有利等位变异,其中降低水SRC的等位变异有gwm642-A186、gwm642-A188和gwm337-A178,降低蔗糖SRC的等位变异有gwm337-A178和gwm337-A186,降低碳酸钠SRC的等位变异有cfa2257-A129等。这些结果为利用分子标记进行SRC辅助选择提供了重要信息。
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