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作物学报 ›› 2015, Vol. 41 ›› Issue (06): 889-899.doi: 10.3724/SP.J.1006.2015.00889

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆叶片性状和叶绿素含量QTL间的上位性和环境互作效应

梁慧珍1,余永亮1,杨红旗1,董薇1,许兰杰1,牛永光1,张海洋1,刘学义2,方宣钧3   

  1. 1 河南省农业科学院芝麻研究中心, 河南郑州 450002; 2 山西省农业科学院经济作物研究所, 山西汾阳 032200; 3 海南省热带农业资源开发利用研究所, 海南三亚 572025
  • 收稿日期:2014-11-13 修回日期:2015-02-06 出版日期:2015-06-12 网络出版日期:2015-03-13
  • 基金资助:

    本研究由河南省科技创新杰出人才计划项目(114200510002), 国家转基因生物新品种培育重大专项(2009ZX08018-001B, 2011ZX08004-005, 2014ZX0800402B), 河南省重点科技攻关计划项目(132102110091)和河南省农业科学院专项(201315603, 201315615, 201218334)资助。

Epistatic and QTL × Environment Interaction Effects of QTLs for Leaf Traits and Leaf Chlorophyll Content in Soybean

LIANG Hui-Zhen1,YU Yong-Liang1,YANG Hong-Qi1,DONG Wei1,XU Lan-Jie1,NIU Yong-Guang1,ZHANG Hai-Yang1,LIU Xue-Yi2,FANG Xuan-Jun3   

  1. 1 Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 2 Industrial Crop Research Institute, Shanxi Academy of Agricultural Sciences, Fenyang 032200, China 3 Hainan Provincial Institute of Tropical Agriculture Resources, Sanya 572025, China
  • Received:2014-11-13 Revised:2015-02-06 Published:2015-06-12 Published online:2015-03-13

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摘要：

大豆；叶片性状；叶绿素含量；QTL与环境互作效应；上位互作效应以丰产性好、抗旱力强的栽培大豆晋豆23 (Jindou 23)为母本，山西农家品种半野生大豆灰布支黑豆为父本杂交衍生的447个RIL作为供试群体，将亲本及447个家系分别于2011、2012和2013年采用随机试验种植，按照标准测量叶长、叶宽和叶柄长3个性状，并于2012年8月1日和8月8日和2013年8月2日和8月9日各测量1次叶绿素含量。采用QTLNETwork 2.0混合线性模型分析方法和主基因+多基因混合遗传分离分析法，对大豆叶片性状和叶绿素含量进行遗传分析和QTL间的上位性和环境互作效应研究。结果表明，叶长受2对加性-加性×加性上位性混合主基因控制，叶宽受3对等效主基因控制，叶柄长受4对加性-加性×加性上位性主基因控制，叶绿素含量受4对加性主基因控制；检测到10个与叶长、叶宽、叶柄长和叶绿素含量相关的QTL，分别位于A1、A2、C2、H_1、L和O染色体。其中2个叶长QTL分别位于C2和L染色体, 是2对加性×加性上位互作效应及环境互作效应QTL；3个叶宽加性与环境互作QTL分别位于A2、C2和O染色体；2个叶柄长QTL分别位于L和O染色体；3个叶绿素含量QTL分别位于A1、C2和H_1染色体。叶片性状和叶绿素含量的遗传机制较复杂，加性效应、加性×加性上位互作效应及环境互作效应是大豆叶片性状和叶绿素含量的重要遗传基础。建议大豆分子标记辅助育种中，一方面要考虑起主要作用的QTL，一方面要注重上位性QTL的影响，这对于性状的遗传和稳定表达具有积极的意义。

关键词: 大豆, 叶片性状, 叶绿素含量, QTL与环境互作效应, 上位互作效应

Abstract:

A RIL population containing 447 lines, derived from a cross of cultivar Jingdou 23 × Huibuzhiheidou, as well as their parents were used to analyze inheritance and detect epistatic effects, and QTL × environment (QE) interactions related to leaf traits and leaf chlorophyll content (CC) in soybean using major gene plus polygene mixed inheritance analysis and composite interval mapping (QTL NETwork 2.0). The leaf traits including leaf length (LL), leaf width (LW), leaf stalk length (LSL) were evaluated in 2011, 2012, and 2013, as well as CC was detected on 1 August and 8 August, 2012, and on 2 August and 9 August, 2013. LL was found to be controlled by two pairs of additive-additive by additive epistatic hybrid main genes, LW was found to be controlled by three pairs of equivalent main genes, LSL was found to be controlled by four pairs of additive-additive by additive epistatic major genes, CC was controlled by four pairs of additive major genes. Ten QTLs for LL, LW, LSL, and CC were mapped on the linkage group (LG) A1, A2, C2, H_1, L, and O, separately. Of them two QTLs for LL were mapped on LG C2 and LG L, additive by additive epistatic effect and QE interactions. Three QTLs with additive effect and QE interactions associated with LW were mapped on LG A2, C2, and O. Two QTLs for LSL were mapped on LG L and O. Three QTLs for CC were mapped on LG A1, C2, and H_1. The genetic mechanism for leaf traits and leaf chlorophyll content is more complicated containing additive effect, additive × additive epistatic effect and QE interaction. It is important to consider not only to QTLs with major effects, but also to those with epistatic effects in soybean molecular marker-assisted breeding for stability of expression and inheritance of agronomic traits.

Key words: Soybean, Leaf traits, Leaf chlorophyll content, QTL ×, environment interactions effects, Epistatic effects

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