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作物学报 ›› 2015, Vol. 41 ›› Issue (10): 1529-1536.doi: 10.3724/SP.J.1006.2015.01529

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

马铃薯块茎形状CAPS标记的开发与验证

朱文文1,徐建飞1,李广存1,段绍光1,刘杰1,卞春松1,庞万福1,Walter De Jong2,金黎平1   

  1. 1中国农业科学院蔬菜花卉研究所，农业部薯类作物生物学和遗传育种重点实验室，北京 100081；2 Department of Plant Breeding and Genetics, Cornell University, Ithaca 14853, NY, USA
  • 收稿日期:2015-03-20 修回日期:2015-07-10 出版日期:2015-10-12 网络出版日期:2015-07-15
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2013AA102603)资助。

Development and Verification of a CAPS Marker Linked to Tuber Shape Gene in Potato

ZHU Wen-Wen1,XU Jian-Fei1,LI Guang-Cun1,DUAN Shao-Guang1,LIU Jie1,BIAN Chun-Song1,PANG Wan-Fu1,Walter De Jong2,JIN Li-Ping1,*   

  1. 1 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture, Beijing 100081, China; 2 Department of Plant Breeding and Genetics, Cornell University, Ithaca 14853, NY, USA
  • Received:2015-03-20 Revised:2015-07-10 Published:2015-10-12 Published online:2015-07-15

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摘要：

马铃薯块茎形状(薯形)是选育马铃薯品种尤其是加工品种最重要的性状之一。前人研究表明薯形是由位于第10染色体的单基因Ro控制的，并且圆形对长形为显性。本研究以二倍体马铃薯长薯形基因型10618-01和圆薯形基因型320-02及其213个F1代薯形分离群体为材料，基于马铃薯基因组相关序列信息结合分离群体分组分析法开发标记，获得了一个CAPS标记1137-CAPSVI。利用该标记对53份不同薯形的四倍体马铃薯高代品系进行验证，结果表明该标记的选择准确率达83.02%，进一步的Pearson双侧相关分析显示其相关性达极显著水平，且该标记对圆薯形材料的选择准确率更高，达91.42%。该CAPS标记的获得对于四倍体马铃薯分子标记辅助育种，加速马铃薯品种尤其是加工专用型品种的选育具有重要意义。

关键词: 马铃薯, 薯形, 标记开发, 分子标记辅助育种

Abstract:

Tuber shape is one of the most important traits for potato cultivars especially for the processing varieties. Previous study shows that a single gene Ro on chromosome 10 controls most variation for round versus long tuber shape. In this study, based on the potato genome sequence information and bulked segregation analysis strategy, we developed a CAPS marker 1137-CAPSVI using the diploid potato genotypes 320-02, 10618-01 and their 213 F1 tuber shape segregated population. This marker was verified in 53 tetraploid breeding lines with diverse tuber shapes. The results showed that the consistency rate between the marker and tuber shape was 83.02% and the accuracy of the CAPS marker 1137-CAPSVI for selecting round tuber shape was up to 91.42%, and the further Pearson correlation analysis result showed that the marker was significantly correlated with tuber shape. The CAPS marker provides a basis for molecular marker-assisted selection in potato breeding especially for processing variety.

Key words: Potato, Tuber shape, Marker development, Molecular marker-assisted breeding

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