SA、MeJA和ACC处理对甘蓝型油菜叶角质层蜡质组分、结构及渗透性的影响

doi:10.3724/SP.J.1006.2016.01827

摘要/Abstract

摘要：

角质层蜡质与植物适应逆境胁迫有关。本研究以甘蓝型油菜中双11为试材，在五叶期分别对其进行200 μmol L^–1水杨酸(SA)溶液、1-氨基环丙烷-1-羧酸(ACC)溶液以及100 μmol L^–1茉莉酸甲酯(MeJA)溶液浇灌处理，分析油菜叶角质层蜡质组分含量、结构以及角质层渗透性的变化。结果表明，MeJA处理7 d后，烷类、二级醇类、酮类、醛类含量以及蜡质总量与对照相比均显著增加，而处理14 d后，所有蜡质组分含量及蜡质总量与对照相比均显著减少；SA与ACC处理早期对叶片蜡质沉积无显著影响(SA处理14 d后，一级醇类、醛类及未知组分含量显著减少)。SA、MeJA和ACC处理21 d后均显著诱导油菜叶片角质层蜡质的沉积，蜡质组分中烷类、酮类、醛类显著增加，其中C29烷、C29酮、C30醛是被SA、MeJA和ACC诱导的主要蜡质组分，暗示烷类、酮类、醛类可能与这些信号分子介导的抗(耐)性反应密切相关。扫描电镜结果显示，SA处理减少叶表皮蜡质杆状结构，且部分区域熔融；MeJA与ACC处理增加油菜叶表皮蜡质的晶体结构密度。角质层蜡质的沉积与结构变化降低角质层渗透性，减缓叶片的水分散失，其中C29烷的特异性增加可能是造成叶片失水率降低的主要原因。

关键词: 甘蓝型油菜, 角质层蜡质, 水杨酸(SA), 茉莉酸甲酯(MeJA), 乙烯(ETH)

Abstract:

Cuticular waxes are related to plant adaptation to environment stress. In the current study, Brassica napus cv. Zhongshuang 11 grown in the soil treated with adding solutions of SA (200 μmol L^–1), MeJA (100 μmol L^–1), and ACC (200 μmol L^–1) wax, were sampled at the five-leaf stage to clarify the effects of SA, MeJA and ETH on adjusting plant cuticular wax deposition. The leaves were used to analyze the amounts of total cuticular wax and wax constituents, wax crystal structure, and cuticular permeability. The amounts of n-alkanes, secondary alcohols, ketones, aldehydes and total cuticular waxes increased significantly when compared with the control at seven days after MeJA treatment, whereas the amounts of all wax constituents and total cuticular waxes significantly decreased at 14 days after MeJA treatment. The application of SA and ACC had no significant influence on cuticular wax deposition at 7 and 14 days after treatments, except for an amount reduction of primary alcohol, aldehyde and unknown constituents for SA treatment at 14 days after treatment. At 21 days after SA, MeJA, and ACC application, the amounts of total cuticular wax, n-alkanes, ketones and aldehyde significantly increased. C29 n-alkane, C29 ketone, and C30 aldehyde were the main cuticular wax constituents induced by SA, MeJA, and ACC application, implying that n-alkane, ketone and aldehyde might be related to the resistance to stresses induced by these signal molecules. Scan electric microscope analysis indicted that the rod-shape structure of cuticular wax in leaf surface reduced and some rods melted under SA treatment. MeJA and ACC application increased the distribution density of wax crystalloids. The cuticular wax deposition and crystal structure alteration reduced the cuticular permeability and delayed the leaf water loss. The specific increase of C29 n-alkane might be the main reason for reducing leaf water loss in B. napus.

Key words: Brassica napus L, Cuticular wax, Salicylic acid, Methyl jasmonic acid, Ethylene

李帅,赵秋棱,彭阳,徐熠,李加纳,倪郁*. SA、MeJA和ACC处理对甘蓝型油菜叶角质层蜡质组分、结构及渗透性的影响[J]. 作物学报, 2016, 42(12): 1827-1833.

LI Shuai,ZHAO Qiu-Ling,PENG Yang,XU Yi,LI Jia-Na,NI Yu*. Effects of SA, MeJA, and ACC on Leaf Cuticular Wax Constituents, Structure and Permeability in Brassica napus[J]. Acta Agron Sin, 2016, 42(12): 1827-1833.

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