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作物学报 ›› 2017, Vol. 43 ›› Issue (01): 133-140.doi: 10.3724/SP.J.1006.2017.00133

• 研究简报 • 上一篇    下一篇

花生栽培种与野生种(Arachis oteroi)人工杂交双二倍体的创制和鉴定

李丽娜1,2,**,杜培2,**,付留洋2,3,刘华2,徐静2,秦利2,严玫2,韩锁义2,黄冰艳2,董文召2   

  1. 1河南科技大学农学院,河南洛阳471023;2河南省农业科学院经济作物研究所 / 农业部黄淮海油料作物重点实验室 / 河南省油料作物遗传改良重点实验室,河南郑州450002;3郑州大学生命科学学院,河南郑州450001
  • 收稿日期:2016-02-29 修回日期:2016-09-18 出版日期:2017-01-12 网络出版日期:2016-09-27
  • 通讯作者: 张新友, E-mail: haasz@126.com, Tel: 0371-65729560
  • 基金资助:

    研究由河南省重大科技专项(141100110600),国家现代农业产业技术体系建设专项(CARS-14)和河南省现代农业产业技术体系项目(S2012-5)资助。

Development and Characterization of Amphidiploid Derived from Interspecific Cross between Cultivated Peanut and Its Wild Relative Arachis oteroi

LI Li-Na1,2,**,DU Pei2,**,FU Liu-Yang2,3,LIU Hua2,XU Jing2,QIN Li2,YAN Mei2,HAN Suo-Yi2,HUANG Bing-Yan2,DONG Wen-Zhao2,TANG Feng-Shou2,ZHANG Xin-You2,*   

  1. 1 College of Agricultural, Henan University of Science and Technology, Luoyang 471023, China; 2 Industrial Crops Research Institute, Henan Academy of Agricultural Sciences / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry of Agriculture / Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou 450002, China; 3 School of life sciences, Zhengzhou University, Zhengzhou 450001, China
  • Received:2016-02-29 Revised:2016-09-18 Published:2017-01-12 Published online:2016-09-27
  • Contact: 张新友, E-mail: haasz@126.com, Tel: 0371-65729560
  • Supported by:

    This study was supported by the Major Technology Research and Development Program of Henan Province (141100110600), the China Agriculture Research System (CARS-14), and the Henan Provincial Agriculture Research System (S2012-05).

摘要:

花生野生种是改良花生栽培种的重要基因资源。为了利用野生花生的抗性基因,本研究利用花生栽培品种豫花9331与二倍体野生种A. oteroi人工杂交,借助胚拯救和染色体秋水仙素加倍,创制一个双二倍体杂种AmE-4,并利用荧光原位杂交和分子标记技术准确鉴定了该双二倍体。观察结果表明,AmE-4的叶片与豫花9331存在显著差异,而主茎高、侧枝长和总分枝数等性状与豫花9331差异不显著。AmE-4开花期较豫花9331推迟60 d,结实性与荚果发育状况较差,不利于AmE-4的育种利用。同时,开发了57个追踪AmE-4中A. oteroi染色体的显性或共显性SSR标记,为创制和鉴定花生栽培种A. oteroi易位系或渐渗系奠定分子基础。

关键词: 花生, 双二倍体, 花生野生种, 分子标记, 荧光原位杂交

Abstract:

Wild Arachis species are important genetic resources. To introgress resistant genes of Arachis species, developed a new amphidiploid AmE-4 through man-made cross between cultivated peanut variety Yuhua 9331 and a diploid Arachis species A. oteroi, with the assistance of following embryo rescue and chromosome doubling by colchicine treatment. AmE-4 was identified and characterized by fluorescence in situ hybridization (FISH) and SSR molecular marker. Morphological observation revealed significant differences in leaves between amphidiploid AmE-4 and Yuhua 9331, while the agronomic traits such as main stem height, length of first lateral branch and number of branches showed less difference between them. The date of first flower appearance in AmE-4 delayed sixty days compared with that in Yuhua 9331, and its pods setting and development were also poor, which would hinder its further utilization. In addition, 57 dominant or co-dominant SSR molecular markers were developed and could be used to identify translocation or introgression lines with A. oteroi chromosome fragment in future studies.

Key words: Peanut (Arachis hypogaea L.), Amphidiploid, Wild Arachis species, Molecular marker, FISH

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