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作物学报 ›› 2017, Vol. 43 ›› Issue (02): 277-285.doi: 10.3724/SP.J.1006.2017.00277

• 耕作栽培·生理生化 • 上一篇    下一篇

套作荫蔽对苗期大豆叶片结构和光合荧光特性的影响

范元芳,杨峰*,刘沁林,谌俊旭,王锐,罗式伶,杨文钰*   

  1. 四川农业大学农学院 / 农业部西南作物生理生态与耕作重点实验室,四川成都 611130
  • 收稿日期:2016-05-17 修回日期:2016-09-18 出版日期:2017-02-12 网络出版日期:2016-09-27
  • 通讯作者: 杨峰, E-mail: f.yang@sicau.edu.cn; 杨文钰, E-mail: mssiyangwy@sicau.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目(31571615), 国家重点研发计划项目(2016YFD0300209)和四川农业大学创新项目(04060658)资助。

Effects of Shading on Leaf Structure and Photosynthetic Fluorescence Characteristics of Soybean seedlings in Maize-Soybean Relay Intercropping System

FAN Yuan-Fang,YANG Feng*,LIU Qin-Lin,CHEN Jun-Xu,WANG Rui,LUO Shi-Ling,YANG Wen-Yu*   

  1. College of Agronomy, Sichuan Agricultural University / Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China.
  • Received:2016-05-17 Revised:2016-09-18 Published:2017-02-12 Published online:2016-09-27
  • Contact: 杨峰, E-mail: f.yang@sicau.edu.cn; 杨文钰, E-mail: mssiyangwy@sicau.edu.cn
  • Supported by:

    The study was supported by the National Natural Science Foundation of China (31571615), the National Key Research and development Program of China (2016YFD0300209), and the Innovation Program of Sichuan Agricultural University ( 04060658).

摘要:

间套作系统中荫蔽是影响低位作物生长发育的主要因素。本研究采用玉米-大豆套作种植模式,以南豆12大豆品种为研究对象,设置2个行比配置,即1∶1(A1处理;1行玉米间隔1行大豆,间距50 cm)和2∶2(A2处理;2行玉米间隔2行大豆,玉米和大豆各自的行距均为40 cm,玉米和大豆间距为60 cm),净作大豆为对照(CK),研究玉米和大豆不同行比套作配置下荫蔽对大豆叶片光合荧光特性、解剖结构及超微结构的影响。结果表明,套作处理A1、A2的光合有效辐射(PAR)分别比CK显著降低91.2%、66.8%。叶绿素a含量分别降低50.2%和27.9%,净光合速率分别降低63.2%和37.8%。套作大豆功能叶片荧光参数Fv'/Fm'和Fq'/Fm显著高于净作大豆,而Fv/FmqP和NPQ低于净作。对于叶片结构,套作大豆叶片厚度、栅栏组织厚度、海绵组织厚度均低于CK,且净作大豆叶片厚度分别是A1和A2处理下的2.15倍和1.69倍;套作大豆叶片的叶绿体结构比较完整,无破碎现象,含淀粉粒稀少,随着荫蔽程度的加重,嗜锇颗粒、基粒片层和基质片层增多,叶绿体减小但其数目增多,而净作大豆叶片叶绿体中淀粉粒较多且色泽亮白,基粒片层和基质片层稀少。因此,玉米-大豆套作种植中不同行比配置导致低位作物大豆冠层光环境差异,直接影响大豆叶片结构特征和光合荧光特性。

关键词: 大豆, 套作, 光合荧光特性, 叶绿体超微结构

Abstract:

Shading in relay intercropping system is a major factor affecting the low growing of crop growth and development. This study attempted to compare leaf structure and photosynthetic characteristics of cultivar Nandou 12 under maize-soybean relay intercropping systems. Three treatments were designed including one row maize to one row soybean with 50 cm of row space between maize and soybean (A1); two rows maize to two rows soybean with 40 cm of row space for maize or soybean and 60 cm of row space between maize and soybean (A2), sole cropping of soybean. The result indicated that shading caused the changes of both anatomical structure of leaf and photosynthetic characteristics in maize/soybean relay strip intercropping systems. During the V5 stage of soybean, the PAR density in treatments (A1 and A2) were lower than that in CK by 91.2% and 66.8%, respectively. The content of Chl a of treatments (A1 and A2) was lower than that in CK by 50.24% and 27.9%. The Pn of treatments (A1 and A2) was lower than that in CK by 63.2% and 37.8%. The Fv/Fm, qP, NPQ in leaf of treatments (A1 and A2) were lower than there in CK significantly, Fv'/Fm' and Fq'/Fm' of treatments (A1 and A2) were higher than that in CK. Under light microscope, compared with CK, A1 and A2 treatments decreased palisade tissue thickness and spongy tissue thickness of leaf. The leaf of treatment CK was 2.15 and 1.69 times as thick as that of treatments A1 and A2. In A1 and A2 treatments leaf chloroplast morphology was normal, the number of chloroplasts, granule lamella and osmophilic globule in chloroplast increased; the structure of chloroplast was not damaged; There were few starch grains in chloroplasts; and the structure of cell organelles was normal. Therefore, the different spatial patterns of maize-soybean intercropping system result in different light environments above the soybean canopy, and directly affect soybean leaf structure and photosynthetic fluorescence characteristics.

Key words: Soybean, Intercropping, Photosynthetic fluorescence characteristics, Chloroplast ultrastructure

[1] 高小丽, 高金锋, 冯佰利, 王鹏科, 柴岩. 不同绿豆品种的叶片解剖结构. 作物学报, 2012, 38: 181–185
Gao X L, Gao J F, Feng B L, Wang P K, Chai Y. Anatomical structure of leaf in different mung bean varieties. Acta Agron Sin, 2012, 38: 181–185 (in Chinese with English abstract)
[2] 冯乃杰, 郑殿峰, 赵玖香, 祖伟, 杜吉到, 张玉先, 梁喜龙. 植物生长物质对大豆叶片形态解剖结构及光合特性的影响. 作物学报. 2009, 35: 1691–1697
Feng N J, Zheng D F, Zhao J X, Zu W, Du J D, Zhang Y X, Liang X L. Effect of plant growth substances on morphological and anatomical structure of leaf and photosynthetic characteristics in soybean. Acta Agron Sin, 2009, 35: 1691–1697
[3] 覃凤飞, 李强, 崔棹茗, 李洪萍, 杨智然. 越冬期遮阴条件下3个不同秋眠型紫花苜蓿品种叶片解剖结构与其光生态适应性. 植物生态学报. 2012, 36: 333–345
Qin F F, Li Q, Cui Z M, Li H P, Yang Z R. Leaf anatomical structures and ecological adaptabilities to light of three alfalfa cultivars with different fall dormancies under shading during overwintering. Chin J Plant Ecol, 2012, 36: 333–345(in Chinese with English abstract)
[4] 甄伟, 张福墁. 弱光对黄瓜功能叶片光合特性及超微结构的影响. 园艺学报, 2000, 27: 290–292
Zhen W, Zhang F M. The effects of low light intensity on photosynthetic characteristics and ultrastructure of cucumber functional leaves. Acta Hort Sin, 2000, 27: 290–292
[5] 马慧丽, 吕德国. 光照条件对“寒富”苹果叶片结构和光合特性的影响. 应用生态学报, 2014, 25: 1927–1932
Ma H L, Lyu D G, Effects of light condition on structure and photosynthetic characteristics of leaves in “Hanfu” apple. Chin J Appl Ecol, 2014,25: 1927–1932 (in Chinese with English abstract)
[6] 黄俊, 郭世荣, 吴震, 李式军. 弱光对不结球白菜光合特性与叶绿体超微结构的影响. 应用生态学报, 2007, 18: 352–358
Huang J, Guo S R, Wu Z, Li S J. Effects of weak light on photosynthetic characteristics and chloroplast ultrastructure of nonheading Chinese cabbage. Chin J Appl Ecol, 2007, 18: 352–358 (in Chinese with English abstract)
[7] 李芳兰, 包维楷. 植物叶片形态解剖结构对环境变化的响应与适应. 植物学通报, 2005, (增刊1): 118–127
Li F L, Bao W K. Responses of the morphological and anatomical structure of the plant leaf to environmental change. Chin Bull Bot, 2005, (suupl-1): 118–127 (in Chinese with English abstract)
[8] 姚允聪, 王绍辉, 孔云. 弱光条件下桃叶片结构及光合特性与叶绿体超微结构变化. 中国农业科学, 2007, 40 : 855–863
Yao Y C, Wang S H, Kong Y. Characteristics of photosynthesis mechanism in different peach species under low light intensity. Sci Agric Sin, 2007: 40: 855–863 (in Chinese with English abstract)
[9] 吴涛, 耿云芬, 柴勇, 郝佳波, 袁春明. 三叶爬山虎叶片解剖结构和光合生理特性对3种生境的响应. 生态环境学报, 2014, 23: 1586–1592
Wu T, Geng Y F, Chai Y, Hao J B, Yuan C M. Response of leaf anatomical structure and photosynthesis characteristics of Parthenocissus himalayana to three habitat types. Ecol Environ Sci, 2014, 23: 1586–1592 (in Chinese with English abstract)
[10] Fan X, Xu Z, Liu X, Liu X Y, Tang C M, Wang L W, Han X L. Effects of light intensity on the growth and leaf development of young tomato plants grown under a combination of red and blue light. Sci Hortic-amsterdam, 2013, 153: 50–55
[11] Jiang C D, Wang X, Gao H Y, Shi L, Wah Soon Chow. Systemic regulation of leaf anatomical structure, photosynthetic performance, and high-light tolerance in sorghum. Plant Physiol, 2011, 155: 1416–1424
[12] 赵团结, 盖钧镒, 李海旺, 邢邯, 邱家驯. 超高产大豆育种研究的进展与讨论. 中国农业科学, 2006, 39: 29–37
Zhao T J, Gai J Y, Li H W, Xing H, Qiu J X. Advances in breeding for super high-yielding soybean cultivars. Sci Agric Sin, 2006, 39: 29–37 (in Chinese with English abstract)
[13] Fehr W R, Caviness C E. Stages of Soybean Development. Special Report 80, Cooperative Extension Service, Agriculture and Home Economic Experiment Station. Ames, Iowa: Iowa State University, 1977. pp 1–11 
[14] 崔亮, 苏本营, 杨峰, 杨文钰. 不同玉米-大豆带状套作组合条件下光合有效辐射强度分布特征对大豆光合特性和产量的影响. 中国农业科学, 2013, 47: 1489–1501
Cui L, Su B Y, Yang F, Yang W Y. Effects of photo-synthetically active radiation on photosynthetic characteristics and yield of soybean in different maize/soybean relay strip intercropping systems. Sci Agric Sin, 2013, 47: 1489–1501 (in Chinese with English abstract)
[15] 李艳大, 汤亮, 张玉屏, 刘蕾蕾, 曹卫星, 朱艳. 水稻冠层光合有效辐射的时空分布特征. 应用生态学报, 2010, 21: 952–958
Li Y D, Tang L, Zhang Y P, Liu L L, Cao W X, Zhu Y. Spatiotemporal distribution of photosynthetically active radiation in rice canopy. Chin J Appl Ecol, 2010, 21: 952–958 (in Chinese with English abstract)
[16] 吕丽华, 赵明, 赵久然, 陶洪斌, 王璞. 不同施氮量下夏玉米冠层结构及光合特性的变化. 中国农业科学, 2008, 41: 2624–2632
Lyu Li H, Zhao M, Zhao J R, Tao H B, Wang P. Canopy structure and photosynthesis of summer maize under different nitrogen fertilizer application rates. Sci Agric Sin, 2008, 41: 2624–2632 (in Chinese with English abstract)
[17] 王锐, 杨峰, 张勇, 黄山, 雍太文, 刘卫国, 杨文钰. 套作大豆后期叶片叶绿素荧光特性及光谱特征分析. 核农学报, 2015, 29: 1182–1189
Wang R, Yang F, Zhang Y, Huang S, Yong T W, Liu W G, Yang W Y. The analysis of chlorophyll fluorescence parameters and hyperspectral characteristics of soybean after maize harvest under relay intercropping systems. Acta Agric Nucl Sin, 2015, 29: 1182–1189 (in Chinese with English abstract)
[18] Arnon D I. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol, 1949, 24: 1–15
[19] 杨虎彪, 李晓霞, 罗丽娟. 植物石蜡制片中透明和脱蜡技术的改良. 植物学报, 2009, 44: 230–235
Yang H B, Li X X, Luo L J. An improved clearing and de-waxing method for plant paraffin sectioning. Chin Bull Bot, 2009, 44: 230–235 (in Chinese with English abstract)
[20] 苏本营, 宋艳霞, 陈圣宾, 杨文钰. 大豆幼苗对套作玉米遮阴环境的光合生理生态响. 生态学报, 2015, 35: 3298–3308
Su B Y, Song Y X, Chen S B, Yang G W Y. Photosynthetic responses of soybean (Glycine max) seedlings to shading caused by maize in an intercropping system. Acta Ecol Sin, 2015, 35: 3298–3308 (in Chinese with English abstract)
[21] 李瑞, 文涛, 唐艳萍, 孙歆, 夏超. 遮阴对大豆幼苗光合和荧光特性的影响. 草业学报, 2014: 198–206
Li R, Wen T, Tang Y P, Sun X, Xia C. Effect of shading on photosynthetic and chlorophyll fluorescence characteristics of soybean. Acta Prat Sin, 2014: 198–206
[22] Demmig-Adams B, Adams W W. The role of xanthophyll cycle carotenoids in the protection of photosynthesis. Trends Plant Sci, 1996, 1: 21–26
[23] Hu W H, Yu J Q. Effects of chilling under low light on photosynthesis and chlorophyll fluorescence characteristic in tomato leaves. Acta Hort Sin, 2001, 28: 41–46
[24] Zhang G R, Du W G, Man W Q, Li G Q, Gui M Z, Wang X D, Ge Q Y, Hao N B. Study on leaf comparative anatomy of different genotypes of Soybean. Chin Bull Bot, 2002, 19: 208–214
[25] Grover A, Mohanty P. Leaf senescence-induced alterations in structure and function of higher plant chloroplasts. In: Abrol Y P, Mohanty P, Govindjee, eds. Photosynthesis: Photoreactions to Plant Productivity. Dordrecht: Kluwer Academic Publishers, 1992. pp 225–255 
[26] Huang D, Wu L, Chen J R, Dong L. Morphological plasticity, photosynthesis and chlorophyll fluorescence of Athyrium pachyphlebium at different shade levels. Photosynthetica, 2011, 49: 611–618
[27] 吴正锋, 孙学武, 王才斌, 郑亚萍, 万书波, 刘俊华, 郑永美, 吴菊香, 冯昊, 于天一. 弱光胁迫对花生功能叶片RuBP羧化酶活性及叶绿体超微结构的影响. 植物生态学报, 2014, 38: 740–748
Wu Z F, Sun X W, Wang C B, Zheng Y P, Wan S B, Liu J H, Zheng Y M, Wu J X, Feng H, Yu T Y. Effects of low light stress on rubisco activity and the ultrastructure of chloroplast in functional leaves of peanut. Acta Ecol Sin, 2014, 38: 740–748 (in Chinese with English abstract)
[28] 艾希珍, 郭延奎, 马兴庄, 邢禹贤. 弱光条件下日光温室黄瓜需光特性及叶绿体超微结构. 中国农业科学, 2004, 37: 268–273
Ai X Z, Guo Y K, Ma X Z, Xing Y X. Photosynthetic characteristics and ultrastructure of chloroplast of cucumber under low light intensity in solar greenhouse. Sci Agric Sin, 2004, 37: 268–273
[29] 刘凡值, 苟兴红, 傅生华, 梁慕勤. 大豆耐阴性的研究: V. 大豆叶片形态特征及解剖结构与耐阴性的关系. 贵州农业科学, 1990, (3): 9–16
Liu F Z, Gou X H, Fu S H, Liang M Q. Studies on shading-endurance of soybean [Glycing max(L.)] Merr.: V. A relation between the morphogical and anatomical characters of soybean leaf and the shade-endurance. Guizhou Agric Sci, 1990, (3): 9–16 (in Chinese with English abstract)
[30] 徐克章, 张治安, 王英典. 光强对人参叶片显微和超微结构的影响. 植物学报, 1994, 11(增刊): 23–27
Xu K Z, Zhang Z A, Wang Y D. Effect of light density on microstructure and ultrastructure of ginseng leaves. Chin Bull Bot, 1994, 11(suppl): 23–27 (in Chinese with English abstract)
[31] 张振贤, 郭延奎, 邹琦. 遮阴对生姜叶片显微结构及叶绿体超微结构的影响. 园艺学报, 1999, 26: 96–100
Zhang Z X, Guo Y K, Zhou Q. Effect of shading on ultrastructure of chloroplast and microstructure of ginger leaves. Acta Hortic Sin, 1999, 26: 96–100 (in Chinese with English abstract)
[32] 魏珉, 邢禹贤, 王秀峰, 马红. CO2加富对黄瓜叶片显微和亚显微结构的影响. 园艺学报, 2002, 29: 30–34
Wei M, Xing Y X, Wang X F, Ma H. Effect of CO2 enrichment on the microstructure and ultrastructure of leaves in cucumber. Acta Hort Sin, 2002, 29: 30–34 (in Chinese with English abstract)

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