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作物学报 ›› 2017, Vol. 43 ›› Issue (07): 1087-1095.doi: 10.3724/SP.J.1006.2017.01087

• 研究简报 • 上一篇    下一篇

利用90k芯片技术进行小麦穗部性状QTL定位

武炳瑾,简俊涛,张德强,马文洁,冯洁,崔紫霞,张传量,孙道杰*   

  1. 西北农林科技大学农学院 陕西杨凌 712100
  • 收稿日期:2016-09-11 修回日期:2017-03-01 出版日期:2017-07-12 网络出版日期:2017-04-06
  • 通讯作者: 孙道杰, E-mail: chinawheat@hotmail.com
  • 基金资助:

    本研究由国家重点基础研究计划(973计划)项目(2014CB138100), 陕西省自然科学基金项目(2015JM3094)和陕西省重点科技创新团队项目(2014KCT-25)资助。

QTL Mapping for Spike Traits of Wheat Using 90k Chip Technology

WU Bing-Jin,JIAN Jun-Tao,ZHANG De-Qiang,MA Wen-Jie,FENG Jie,CUI Zi-Xia,ZHANG Chuan-Liang,SUN Dao-Jie*   

  1. College of Agronomy, Northwest A&F University, Yangling 712100, China
  • Received:2016-09-11 Revised:2017-03-01 Published:2017-07-12 Published online:2017-04-06
  • Contact: Sun Daojie, E-mail: chinawheat@hotmail.com
  • Supported by:

    This study was supported by the National Key Basic Research Program of China (2014CB138100), the Natural Science Foundation of Shaanxi Province (2015JM3094), and the Key Scientific and Technological Innovation Team of Shaanxi Province (2014KCT-25).

摘要:

小麦穗部性状与产量密切相关,挖掘穗部性状基因及其关联分子标记具有重要意义。本研究以周8425B?小偃81衍生的RIL群体(F8)为材料,利用90k芯片标记构建的高密度遗传图谱对3个环境下的穗长、小穗数、不育小穗数、穗粒数、千粒重进行QTL定位。共检测到19条染色体上的71个QTL,变异解释率(PVE)范围为2.10%~45.25%,其中37个位点为主效QTL (PVE>10%)。QSl.nafu-6A.2 (穗长)、QSl.nafu-7A (穗长)、QSsn.nafu-2A.1 (小穗数)、QSsn.nafu-2D (小穗数)和QGns.nafu-2B (穗粒数)在多个环境中被检测到,且LOD>10,PVE>20%。位于同一个基因簇中的QSl.nafu-6A.2 (穗长)、QGns.nafu-6A (穗粒数)和QTgw.nafu-6A (千粒重)在多个环境中被检测到,且与已报道的相关位点位置相同或相近,在分子标记辅助育种中具有较大参考价值。

关键词: 小麦, 穗部性状, 90k基因芯片, QTL定位

Abstract:

Spike traits are important to grain yield in wheat. Molecular markers associated with genes/QTLs controlling spike traits are highly valuable to marker-assisted breeding. A recombinant inbred line (F8) population derived from Zhou 8425B ? Xiaoyan 81 were evaluated in three environments, and QTLs for spike length, spikelet number per spike, sterile spikelet number, grain number per spike and thousand-grain weigh were mapped into a high-density genetic map built by 90k chip. A total of 71 QTLs were located on 19 chromosomes, and the phenotype variation explained (PVE) by a single locus ranged from 2.10% to 45.25%. Thirty-seven loci were considered as main-effect QTLs owing to the PVE larger than 10%. QTLs QSl.nafu-6A.2 for spike length, QSl.nafu-7A for spike length, QSsn.nafu-2A.1 for sterile spikelet number, QSsn.nafu-2D for sterile spikelet number and QGns.nafu-2B for grain number per spike were identified repeatedly in different environments with the LOD value higher than 10 and PVE larger than 20%. QSl.nafu-6A.2 for spike length, QGns.nafu-6A for grain number per spike and QTgw.nafu-6A for thousand-grain weight were mapped in a cluster on chromosome 6A and might be applicable in marker-assisted selection because they have been detected in multiple environments and close to the loci reported.

Key words: Triticum aestivum, Spike-related traits, 90k gene chip, QTL mapping

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