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作物学报 ›› 2018, Vol. 44 ›› Issue (8): 1212-1220.doi: 10.3724/SP.J.1006.2018.01212

• 耕作栽培·生理生化 • 上一篇    下一篇

CO2浓度对大豆叶片气孔特征和气体交换参数的影响

李菲1(),刘亮1,张浩2,王清涛3,郭丽丽1,郝立华1,*(),张茜茜1,曹旭1,梁伟佳1,郑云普1,*()   

  1. 1 河北工程大学水利水电学院, 河北邯郸 056038
    2 邯郸学院生命科学与工程学院, 河北邯郸 056005
    3 河北工程大学园林与生态工程学院, 河北邯郸 056038
  • 收稿日期:2017-12-22 接受日期:2018-04-11 出版日期:2018-08-10 网络出版日期:2018-05-11
  • 通讯作者: 郝立华,郑云普
  • 基金资助:
    国家自然科学基金项目(31400418);河北省自然科学基金项目(C2016402088);河北省自然科学基金项目(E2016402098);河北省创新能力提升计划科技研发平台建设专项“河北省水资源高效利用工程技术研究中心”(18965307H)(Research Center for High-efficiency Utilization of Water Resources);河北省创新能力提升计划科技研发平台建设专项“河北省水资源高效利用工程技术研究中心”(18965307H);河北省高等学校青年拔尖人才计划项目(BJ2016012);河北省引进留学人员资助项目(CN201702);中国博士后科学基金项目(2014M561044);中国博士后科学基金项目(2016T90128);河北省教育厅青年科学基金项目(QN2015253)

Effects of CO2 Concentrations on Stomatal Traits and Gas Exchange in Leaves of Soybean

Fei LI1(),Liang LIU1,Hao ZHANG2,Qing-Tao WANG3,Li-Li GUO1,Li-Hua HAO1,*(),Xi-Xi ZHANG1,Xu CAO1,Wei-Jia LIANG1,Yun-Pu ZHENG1,*()   

  1. 1 School of Water Conservancy and Hydroelectric Power, Hebei University of Engineering, Handan 056038, Hebei, China
    2 School of Life Science and Engineering, Handan University, Handan 056005, Hebei, China
    3 School of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056038, Hebei, China
  • Received:2017-12-22 Accepted:2018-04-11 Published:2018-08-10 Published online:2018-05-11
  • Contact: Li-Hua HAO,Yun-Pu ZHENG
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31400418);the Natural Science Foundation of Hebei Province(C2016402088);the Natural Science Foundation of Hebei Province(E2016402098);the Innovation Capability Upgrading Plan of Hebei Province(Research Center for High-efficiency Utilization of Water Resources);the Innovation Capability Upgrading Plan of Hebei Province (Research Center for High-efficiency Utilization of Water Resources)(18965307H);the Young Outstanding Innovative Talents of Hebei Province(BJ2016012);the Foundation for Returnees of Hebei Province(CN201702);China Postdoctoral Science Foundation Funded Projects(2014M561044);China Postdoctoral Science Foundation Funded Projects(2016T90128);the Science and Technology Research Project of Hebei Colleges and Universities(QN2015253)

摘要:

利用可精准控制CO2浓度的大型气候箱设置7个CO2浓度处理(400、600、800、1000、1200、1400和1600 μmol mol -1), 对大豆进行CO2浓度富集的室内培养试验。结果表明, CO2浓度升高显著减小大豆叶片近轴面的气孔密度和远/近轴面的气孔面积指数。当CO2浓度为400 μmol mol -1时, 远轴面气孔分布最规则, 提高CO2浓度导致远轴面气孔的不规则分布; 与远轴面相反, CO2浓度升高导致近轴面气孔的空间分布更加规则, 即在较高CO2浓度处理下的Lhat(d)最小值均低于对照组。不同叶面(远/近轴面)气孔特征对大气CO2浓度变化的响应存在明显差异, 但大豆可以通过调整气孔形态特征和气孔空间分布格局进一步改变叶片的气体交换参数。研究结果有助于从气孔特征响应的角度深入理解CO2浓度对大豆叶片气体交换过程产生的影响。

关键词: CO2浓度, 大豆, 气孔结构特征, 气孔空间分布, 气体交换参数

Abstract:

Seven concentrations treatments (400, 600, 800, 1000, 1200, 1400, and 1600 μmol mol -1) were designed to investigate the effects of atmospheric CO2 concentrations on the stomatal traits and leaf gas exchange of soybean. We found that elevating CO2 concentrations significantly decreased the stomatal density of adaxial side and the stomatal area index of both the adaxial and abaxial sides. Meanwhile, the spatial distribution pattern analysis of stomata with the Ripley’s K function showed that the spatial distribution pattern of stomata on leaf surfaces of soybean was highly scale-dependent. The most regular distribution pattern of stomata on the abaxial surface was found under the CO2 concentration of 400 μmol mol -1, and the increase of CO2 concentration resulted in irregular distribution pattern of stomata on the abaxial surface of soybean leaves. In contrast to the abaxial surface, elevating CO2 concentrations made the spatial distribution pattern of stomata more regular on the adaxial leaf surface, which was evidenced by lower minimal Lhat(d) values under elevated CO2 concentrations than those under CO2 concentration of 400 μmol mol -1. Although the response of stomatal traits to atmospheric CO2 concentration was obviously different between the adaxial and abaxial surfaces of leaves, soybean plants could alter leaf gas exchange through adjusting the morphological traits and the spatial distribution pattern of stomata. These results may be helpful for further understanding potential mechanisms concerning about the elevating CO2 effect on the leaf gas exchange of soybean plants from the view of stomatal traits.

Key words: CO2 concentration, soybean plants, stomatal structure and function, stomatal distribution pattern, leaf gas exchange

表1

CO2浓度对气孔密度和单个气孔形态特征的影响"

图2

不同CO2浓度下大豆叶片近轴面和远轴面气孔扫描电子显微照片 CO2浓度为400、600、800、1000、1200、1400和1600 μmol mol-1环境下大豆近轴面(a~g)和远轴面的气孔形态特征(A~G)。"

表2

CO2浓度对大豆不同叶面气孔参数的交互作用"

参数
Parameter
气孔密度
Stomatal density
(No. mm-2)
气孔长度
Stomatal length
(mm)
气孔宽度
Stomatal width
(mm)
气孔面积
Stomatal area
(mm2)
气孔周长
Stomatal
perimeter (mm)
气孔面积指数
Stomatal area index (%)
气孔形状指数
Stomatal shape index (%)
CO2 0.149 0.531 0.352 0.102 0.910 0.194 0.130
叶面 Surface <0.001 <0.001 <0.001 <0.001 0.805 0.933 0.002
CO2×叶面 CO2×surface 0.009 0.158 0.350 0.006 0.269 0.323 0.290

图1

CO2浓度对大豆叶片气孔空间分布格局的影响 大豆叶片近轴面(a)和远轴面(b)气孔空间分布格局。"

图3

CO2浓度对大豆叶片气孔导度、蒸腾速率和水分利用效率的影响 (a)大豆叶片气孔导度; (b)蒸腾速率; (c)水分利用效率。"

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