欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2018, Vol. 44 ›› Issue (8): 1212-1220.doi: 10.3724/SP.J.1006.2018.01212

• 耕作栽培·生理生化 • 上一篇    下一篇

CO2浓度对大豆叶片气孔特征和气体交换参数的影响

李菲1(),刘亮1,张浩2,王清涛3,郭丽丽1,郝立华1,*(),张茜茜1,曹旭1,梁伟佳1,郑云普1,*()   

  1. 1 河北工程大学水利水电学院, 河北邯郸 056038
    2 邯郸学院生命科学与工程学院, 河北邯郸 056005
    3 河北工程大学园林与生态工程学院, 河北邯郸 056038
  • 收稿日期:2017-12-22 接受日期:2018-04-11 出版日期:2018-08-10 网络出版日期:2018-05-11
  • 通讯作者: 郝立华,郑云普
  • 基金资助:
    国家自然科学基金项目(31400418);河北省自然科学基金项目(C2016402088);河北省自然科学基金项目(E2016402098);河北省创新能力提升计划科技研发平台建设专项“河北省水资源高效利用工程技术研究中心”(18965307H)(Research Center for High-efficiency Utilization of Water Resources);河北省创新能力提升计划科技研发平台建设专项“河北省水资源高效利用工程技术研究中心”(18965307H);河北省高等学校青年拔尖人才计划项目(BJ2016012);河北省引进留学人员资助项目(CN201702);中国博士后科学基金项目(2014M561044);中国博士后科学基金项目(2016T90128);河北省教育厅青年科学基金项目(QN2015253)

Effects of CO2 Concentrations on Stomatal Traits and Gas Exchange in Leaves of Soybean

Fei LI1(),Liang LIU1,Hao ZHANG2,Qing-Tao WANG3,Li-Li GUO1,Li-Hua HAO1,*(),Xi-Xi ZHANG1,Xu CAO1,Wei-Jia LIANG1,Yun-Pu ZHENG1,*()   

  1. 1 School of Water Conservancy and Hydroelectric Power, Hebei University of Engineering, Handan 056038, Hebei, China
    2 School of Life Science and Engineering, Handan University, Handan 056005, Hebei, China
    3 School of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056038, Hebei, China
  • Received:2017-12-22 Accepted:2018-04-11 Published:2018-08-10 Published online:2018-05-11
  • Contact: Li-Hua HAO,Yun-Pu ZHENG
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31400418);the Natural Science Foundation of Hebei Province(C2016402088);the Natural Science Foundation of Hebei Province(E2016402098);the Innovation Capability Upgrading Plan of Hebei Province(Research Center for High-efficiency Utilization of Water Resources);the Innovation Capability Upgrading Plan of Hebei Province (Research Center for High-efficiency Utilization of Water Resources)(18965307H);the Young Outstanding Innovative Talents of Hebei Province(BJ2016012);the Foundation for Returnees of Hebei Province(CN201702);China Postdoctoral Science Foundation Funded Projects(2014M561044);China Postdoctoral Science Foundation Funded Projects(2016T90128);the Science and Technology Research Project of Hebei Colleges and Universities(QN2015253)

RichHTML

10

PDF

784

PDF

25

摘要：

利用可精准控制CO2浓度的大型气候箱设置7个CO2浓度处理(400、600、800、1000、1200、1400和1600 μmol mol -1), 对大豆进行CO2浓度富集的室内培养试验。结果表明, CO2浓度升高显著减小大豆叶片近轴面的气孔密度和远/近轴面的气孔面积指数。当CO2浓度为400 μmol mol -1时, 远轴面气孔分布最规则, 提高CO2浓度导致远轴面气孔的不规则分布; 与远轴面相反, CO2浓度升高导致近轴面气孔的空间分布更加规则, 即在较高CO2浓度处理下的Lhat(d)最小值均低于对照组。不同叶面(远/近轴面)气孔特征对大气CO2浓度变化的响应存在明显差异, 但大豆可以通过调整气孔形态特征和气孔空间分布格局进一步改变叶片的气体交换参数。研究结果有助于从气孔特征响应的角度深入理解CO2浓度对大豆叶片气体交换过程产生的影响。

关键词: CO2浓度, 大豆, 气孔结构特征, 气孔空间分布, 气体交换参数

Abstract:

Seven concentrations treatments (400, 600, 800, 1000, 1200, 1400, and 1600 μmol mol -1) were designed to investigate the effects of atmospheric CO2 concentrations on the stomatal traits and leaf gas exchange of soybean. We found that elevating CO2 concentrations significantly decreased the stomatal density of adaxial side and the stomatal area index of both the adaxial and abaxial sides. Meanwhile, the spatial distribution pattern analysis of stomata with the Ripley’s K function showed that the spatial distribution pattern of stomata on leaf surfaces of soybean was highly scale-dependent. The most regular distribution pattern of stomata on the abaxial surface was found under the CO2 concentration of 400 μmol mol -1, and the increase of CO2 concentration resulted in irregular distribution pattern of stomata on the abaxial surface of soybean leaves. In contrast to the abaxial surface, elevating CO2 concentrations made the spatial distribution pattern of stomata more regular on the adaxial leaf surface, which was evidenced by lower minimal Lhat(d) values under elevated CO2 concentrations than those under CO2 concentration of 400 μmol mol -1. Although the response of stomatal traits to atmospheric CO2 concentration was obviously different between the adaxial and abaxial surfaces of leaves, soybean plants could alter leaf gas exchange through adjusting the morphological traits and the spatial distribution pattern of stomata. These results may be helpful for further understanding potential mechanisms concerning about the elevating CO2 effect on the leaf gas exchange of soybean plants from the view of stomatal traits.

Key words: CO2 concentration, soybean plants, stomatal structure and function, stomatal distribution pattern, leaf gas exchange

表1

CO2浓度对气孔密度和单个气孔形态特征的影响"

图2

不同CO2浓度下大豆叶片近轴面和远轴面气孔扫描电子显微照片 CO2浓度为400、600、800、1000、1200、1400和1600 μmol mol-1环境下大豆近轴面(a~g)和远轴面的气孔形态特征(A~G)。"

表2

CO2浓度对大豆不同叶面气孔参数的交互作用"

参数
Parameter		 气孔密度
Stomatal density
(No. mm-2)		 气孔长度
Stomatal length
(mm)		 气孔宽度
Stomatal width
(mm)		 气孔面积
Stomatal area
(mm2)		 气孔周长
Stomatal
perimeter (mm)		 气孔面积指数
Stomatal area index (%)		 气孔形状指数
Stomatal shape index (%)
CO2 0.149 0.531 0.352 0.102 0.910 0.194 0.130
叶面 Surface <0.001 <0.001 <0.001 <0.001 0.805 0.933 0.002
CO2×叶面 CO2×surface 0.009 0.158 0.350 0.006 0.269 0.323 0.290

图1

CO2浓度对大豆叶片气孔空间分布格局的影响 大豆叶片近轴面(a)和远轴面(b)气孔空间分布格局。"

图3

CO2浓度对大豆叶片气孔导度、蒸腾速率和水分利用效率的影响 (a)大豆叶片气孔导度; (b)蒸腾速率; (c)水分利用效率。"

[1] IPCC. Intergovernmental panel on climate change (2013) summary for policymakers. In: Stocker T F, Qin D, Plattner G K, Tignor M, Allen S K, Bo-schung J, Nauels A, Xia Y, Bex V, Midgley P M, eds. Climate Change 2013: The Physical Science Basis, Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge: Cambridge University Press, pp 225-248.
[2] Kim H Y, Lieffering M, Kobayashi K, Okada M, Shu M . Seasonal changes in the effects of elevated CO2 on rice at three levels of nitrogen supply: a free air CO2 enrichment (FACE) experiment. Global Change Biol, 2003,9:826-837
[3] 李萍, 郝兴宇, 杨宏斌, 林而达 . 大气CO2浓度升高对绿豆生长发育与产量的影响. 核农学报, 2011,25:358-362
Li P, Hao X Y, Yang H B, Lin E D . Effects of air CO2 enrichment on growth and yield of mung bean. J Nucl Agric Sci, 2011,25:358-362 (in Chinese with English abstract)
[4] 于显枫, 张绪成, 王红丽 . 高浓度CO2下氮素对小麦叶片干物质积累及碳氮关系的影响. 核农学报, 2012,26:1058-1063
Yu X F, Zhang X C, Wang H L . Effects of nitrogen on the dry matter accumulation, carbon and nitrogen metabolism of wheat leaves under elevated atmospheric CO2 concentration. J Nucl Agric Sci, 2012,26:1058-1063 (in Chinese with English abstract)
[5] Bowes G . Facing the inevitable: plants and increasing atmospheric CO2. Annu Rev Plant Physiol Plant Mol Biol, 1993,44:309-332
doi: 10.1146/annurev.pp.44.060193.001521
[6] Woodward F I . Stomatal numbers are sensitive to increases in CO2 from preindustrial levels. Nature, 1987,327:617-618
doi: 10.1038/327617a0
[7] Hetheringto A M, Woodward F I . The role of stomata in sensing and driving environmental change. Nature, 2003, 424:901-908
doi: 10.1038/nature01843 pmid: 12931178
[8] 王慧, 周广胜, 蒋延玲, 石耀辉, 许振柱 . 降水与CO2浓度协同作用对短花针茅光合特性的影响. 植物生态学报, 2012,36:597-606
Wang H, Zhou G S, Jiang Y L, Shi Y H, Xu Z Z . Interactive effects of changing precipitation and elevated CO2 concentration on photosynthetic parameters of Stipa breviflora. Chin J Plant Ecol, 2012,36:597-606 (in Chinese with English abstract)
[9] 孙谷畴, 赵平, 彭少麟, 曾小平 . 在高CO2浓度下四种亚热带幼树光合作用对水分胁迫的响应. 生态学报, 2001,21:738-746
Sun G C, Zhao P, Peng S L, Zeng X P . Response of photosynthesis to water stress in four saplings from subtropical forests under elevated atmospheric CO2 concentration. Acta Ecol Sin, 2001,21:738-746 (in Chinese with English abstract)
[10] 王建林, 温学发, 赵风华, 房全孝, 杨新民 . CO2浓度倍增对8种作物叶片光合作用、蒸腾作用和水分利用效率的影响. 植物生态学报, 2012,6:438-446
doi: 10.3724/SP.J.1258.2012.00438
Wang J L, Wen X F, Zhao F H, Fang Q X, Yang X M . Effects of doubled CO2 concentration on leaf photosynthesis, transpiration and water use efficiency of eight crop species. Chin J Plant Ecol, 2012,6:438-446 (in Chinese with English abstract)
doi: 10.3724/SP.J.1258.2012.00438
[11] Farquhar G D, Von Caemmerer S, Berry J A . Models of photosynthesis. Plant Physiol, 2001,125:42-45
doi: 10.1104/pp.125.1.42
[12] Reeves D W, Rogers H H, Prior S A, Wood C W, Runion G B . Elevated atmospheric carbon dioxide effects on sorghum and soybean nutrient status. J Plant Nutr, 1994,17:1939-1954
doi: 10.1080/01904169409364856
[13] 翟志席, 郭玉海, 马永泽, 柏长青 . 植物生态生理学. 北京: 中国农业大学出版社, 1997, pp 60-66
Zhai Z X, Guo Y H, Ma Y Z, Bai C Q . Plant Ecophysiology. Beijing: China Agricultural University Press, 1997. pp 60-66(in Chinese)
[14] Apple M E, Olszyk D M, Ormrod D P, Lewis J, Southworth D, Tinqey D T . Morphology and stomatal function of douglas fir needles exposed to climate change: elevated CO2 and temperature. Int J Plant Sci, 2000,161:127-132
doi: 10.1086/314237
[15] Kouwenberg L L R, Kurschner W M, Mcelwain J C . Stomatal frequency change over altitudinal gradients: prospects for paleoaltimetry. Rev Mineral Geochem, 2007,66:215-241
doi: 10.2138/rmg.2007.66.9
[16] Fraser L H, Greenall A, Carlyle C, Turkington R, Friedman C R . Adaptive phenotypic plasticity of Pseudoroegneria spicata: response of stomatal density, leaf area and biomass to changes in water supply and increased temperature. Ann Bot, 2009,103:769-775
[17] Polly H W, Johnson H B, Mayeux H S . Carbon dioxide and water fluxes of C3 and C4 perennials at subambient CO2 concentrations. Funct Ecol, 1992,6:693-703
doi: 10.2307/2389966
[18] Xu M . The optimal atmospheric CO2 concentration for the growth of winter wheat. J Plant Physiol, 2015,184:89-97
doi: 10.1016/j.jplph.2015.07.003 pmid: 26253981
[19] Croxdale J L . Stomatal patterning in angiosperms. J Bot, 2000,87:1069-1080
doi: 10.2307/2656643
[20] Shpak E D, Mcabee J M, Pillitteri L J, Ku T . Stomatal patterning and differentiation by synergistic interactions of receptor kinases. Science, 2005,309:290-293
doi: 10.1126/science.1109710
[21] 孙成明, 庄恒扬, 杨连新, 杨洪建, 黄建晔, 董桂春, 朱建国, 王余龙 . FACE水稻生育期模拟. 生态学报, 2007,27:613-619
doi: 10.3321/j.issn:1000-0933.2007.02.024
Sun C M, Zhuang H Y, Yang L X, Yang H J, Huang J Y, Dong G C, Zhu J G, Wang Y L . A simulation of growth duration FACE rice. Acta Ecol Sin, 2007,27:613-619 (in Chinese with English abstract)
doi: 10.3321/j.issn:1000-0933.2007.02.024
[22] 蒋跃林, 张庆国, 岳伟, 姚玉刚, 王公明 . 大气CO2浓度升高对大豆生长和产量的影响. 中国农学通报, 2005,21:355-357
doi: 10.3969/j.issn.1000-6850.2005.06.105
Jiang Y L, Zhang Q G, Yue W, Yao Y G, Wang G M . Effects of elevated atmospheric CO2 concentration on growth and yield of soybean. Chin Agric Sci Bull, 2005,21:355-357 (in Chinese with English abstract)
doi: 10.3969/j.issn.1000-6850.2005.06.105
[23] 王修兰, 徐师华 . CO2浓度倍增对大豆各生育期阶段的光合作用及干物质积累的影响. 作物学报, 1994,20:520-527
Wang X L, Xu S H . Effect of CO2 concentration doubling on photosynthesis and dry matter production in different growth stages of soybean plant. Acta Agron Sin, 1994,20:520-527 (in Chinese with English abstract)
[24] Zheng Y P, Xu M, Hou R X, Shen R C, Qiu S, Ou-Yang Z . Effects of experimental warming on stomatal traits in leave s of maize (Zea may L.). Ecol Evol, 2013,3:3095-3111
doi: 10.1002/ece3.674 pmid: 3790554
[25] 张绪成, 于显枫, 高世铭 . 高大气CO2浓度下氮素对小麦叶片光能利用的影响. 植物生态学报, 2010,34:1196-1203
Zhang X C, Yu X F, Gao S M . Effects of nitrogen application rates on photosynthetic energy utilization in wheat leaves under elevated atmospheric CO2 concentration. Chin J Plant Ecol, 2010,34:1196-1203 (in Chinese with English abstract)
[26] 张绪成, 于显枫, 马一凡, 上官周平 . 高大气CO2浓度下小麦旗叶光合能量利用对氮素和光强的响应. 生态学报, 2011,31:1046-1057
Zhang X C, Yu X F, Ma Y F , Shang-Guan Z P. The responses of photosynthetic energy use in wheat flag leaves to nitrogen application rates and light density under elevated atmospheric CO2 concentration. Acta Ecol Sin, 2011,31:1046-1057 (in Chinese with English abstract)
[27] Teng N J, Wang J, Chen T, Wang Y, Lin J . Elevated CO2 induces physiological, biochemical and structural changes in leaves of Arabidopsis thaliana. New Phytol, 2006,172:92-103
[28] Kruse J, Hetzger I, Mai C, Polle A, Rennenberg H . Elevated CO2 affects N-metabolism of young poplar plants (Populus tremula × P. alba) differently at deficient and sufficient N-supply. New Phytol, 2003,157:65-81
[29] 朱玉, 黄磊, 党承华, 王贺新, 姜国斌, 李根柱, 张子川, 娄鑫, 郑云普 . 高温对蓝莓叶片气孔特征和气体交换参数的影响. 农业工程学报, 2016,32(1):218-225
Zhu Y, Huang L, Dang C H, Wang H X, Jiang G B, Li Y Z, Zhang Z C, Lou X, Zheng Y P . Effects of high temperature on leaf stomatal traits and gas exchange parameters of blueberry. Trans CSAE, 2016,32(1):218-225 (in Chinese with English abstract)
[30] 郑云普, 徐明, 王建书, 王贺新 . 气候变暖对华北平原玉米叶片形态结构和气体交换过程的影响. 生态学报, 2016,36:1526-1538
doi: 10.5846/stxb201408231669
Zheng Y P, Xu M, Wang J S, Wang H X . Effects of future climate warming on the morphology, structure, and gas exchange of maize leaves in the North China Plain. Acta Ecol Sin, 2016,36:1526-1538 (in Chinese with English abstract)
doi: 10.5846/stxb201408231669
[31] Amthor J S . Effects of atmospheric CO2 concentration on wheat yield: review of results from experiments using various approaches to control CO2 concentration. Field Crops Res, 2001,84:1-34
[32] Cotrufo M F, Ineson P, Scott A . Elevated CO2 reduces the nitrogen concentration of plant tissues. Global Change Biol, 1998,4:43-54
doi: 10.1046/j.1365-2486.1998.00101.x
[33] Donohue R J, Roderick M L, McVicar T R, Farquhar G D . Impact of CO2 fertilization on maximum foliage cover across the globe’s warm arid environments. Geophys Res Lett, 2013,40:3031-3035
[34] Field C B, Jackson R B, Mooney H A . Stomatal responses to increased CO2: implications from the plant to the global scale. Plant Cell Environ, 1995,18:1214-1225
doi: 10.1111/j.1365-3040.1995.tb00630.x
[35] JablonskiL M, Wang X, Curtis P S . Plant reproduction under elevated CO2 conditions: a meta-analysis of reports on 79 crop and wild species. New Phytol, 2002,156:9-26
[36] Valle R M . Transpiration rate and water use efficiency of soybean leaves adapted to different CO2 environments. Crop Sci, 1985,25:47-482
doi: 10.1016/0261-2194(85)90058-4
[37] Levine L H, Richards J T, Wheeler R M . Super-elevated CO2 interferes with stomatal response to ABA and night closure in soybean (Glycine max). J Plant Physiol, 2009,166:903-913
[38] Bunce J A . Stomatal conductance, photosynthesis and respiration of temperate deciduous tree seedlings grown outdoors at an elevated concentration of carbon dioxide. Plant Cell Environ, 1992,15:541-549
doi: 10.1111/pce.1992.15.issue-5
[39] Assmann S M, Shimazaki K I . The multisensory guard cell: stomatal responses to blue light and abscisic acid. J Plant Physiol, 1999,119:809-815
doi: 10.1104/pp.119.3.809
[40] Kolla V A, Vavasseur A, Raghavendra A S . Hydrogen peroxide production is an early event during bicarbonate induced stomatal closure in abaxial epidermis of Arabidopsis. Planta, 2007,225:1421-1429
doi: 10.1007/s00425-006-0450-6
[41] Morison J I L . Intercellular CO2 concentration and stomatal response to CO2. In: Zeiger E, Cowan I R, Farquhar G D, eds. Stomatal Function. California: Stanford University Press, 1987. pp 229-512
[1] 陈玲玲, 李战, 刘亭萱, 谷勇哲, 宋健, 王俊, 邱丽娟. 基于783份大豆种质资源的叶柄夹角全基因组关联分析[J]. 作物学报, 2022, 48(6): 1333-1345.
[2] 杨欢, 周颖, 陈平, 杜青, 郑本川, 蒲甜, 温晶, 杨文钰, 雍太文. 玉米-豆科作物带状间套作对养分吸收利用及产量优势的影响[J]. 作物学报, 2022, 48(6): 1476-1487.
[3] 王炫栋, 杨孙玉悦, 高润杰, 余俊杰, 郑丹沛, 倪峰, 蒋冬花. 拮抗大豆斑疹病菌放线菌菌株的筛选和促生作用及防效研究[J]. 作物学报, 2022, 48(6): 1546-1557.
[4] 于春淼, 张勇, 王好让, 杨兴勇, 董全中, 薛红, 张明明, 李微微, 王磊, 胡凯凤, 谷勇哲, 邱丽娟. 栽培大豆×半野生大豆高密度遗传图谱构建及株高QTL定位[J]. 作物学报, 2022, 48(5): 1091-1102.
[5] 李阿立, 冯雅楠, 李萍, 张东升, 宗毓铮, 林文, 郝兴宇. 大豆叶片响应CO2浓度升高、干旱及其交互作用的转录组分析[J]. 作物学报, 2022, 48(5): 1103-1118.
[6] 彭西红, 陈平, 杜青, 杨雪丽, 任俊波, 郑本川, 罗凯, 谢琛, 雷鹿, 雍太文, 杨文钰. 减量施氮对带状套作大豆土壤通气环境及结瘤固氮的影响[J]. 作物学报, 2022, 48(5): 1199-1209.
[7] 王好让, 张勇, 于春淼, 董全中, 李微微, 胡凯凤, 张明明, 薛红, 杨梦平, 宋继玲, 王磊, 杨兴勇, 邱丽娟. 大豆突变体ygl2黄绿叶基因的精细定位[J]. 作物学报, 2022, 48(4): 791-800.
[8] 李瑞东, 尹阳阳, 宋雯雯, 武婷婷, 孙石, 韩天富, 徐彩龙, 吴存祥, 胡水秀. 增密对不同分枝类型大豆品种同化物积累和产量的影响[J]. 作物学报, 2022, 48(4): 942-951.
[9] 杜浩, 程玉汉, 李泰, 侯智红, 黎永力, 南海洋, 董利东, 刘宝辉, 程群. 利用Ln位点进行分子设计提高大豆单荚粒数[J]. 作物学报, 2022, 48(3): 565-571.
[10] 周悦, 赵志华, 张宏宁, 孔佑宾. 大豆紫色酸性磷酸酶基因GmPAP14启动子克隆与功能分析[J]. 作物学报, 2022, 48(3): 590-596.
[11] 王娟, 张彦威, 焦铸锦, 刘盼盼, 常玮. 利用PyBSASeq算法挖掘大豆百粒重相关位点与候选基因[J]. 作物学报, 2022, 48(3): 635-643.
[12] 董衍坤, 黄定全, 高震, 陈栩. 大豆PIN-Like (PILS)基因家族的鉴定、表达分析及在根瘤共生固氮过程中的功能[J]. 作物学报, 2022, 48(2): 353-366.
[13] 张国伟, 李凯, 李思嘉, 王晓婧, 杨长琴, 刘瑞显. 减库对大豆叶片碳代谢的影响[J]. 作物学报, 2022, 48(2): 529-537.
[14] 宋丽君, 聂晓玉, 何磊磊, 蒯婕, 杨华, 郭安国, 黄俊生, 傅廷栋, 汪波, 周广生. 饲用大豆品种耐荫性鉴定指标筛选及综合评价[J]. 作物学报, 2021, 47(9): 1741-1752.
[15] 曹亮, 杜昕, 于高波, 金喜军, 张明聪, 任春元, 王孟雪, 张玉先. 外源褪黑素对干旱胁迫下绥农26大豆鼓粒期叶片碳氮代谢调控的途径分析[J]. 作物学报, 2021, 47(9): 1779-1790.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2