作物学报 ›› 2019, Vol. 45 ›› Issue (4): 508-521.doi: 10.3724/SP.J.1006.2019.83060
张春宵1,李淑芳1,金峰学1,刘文平1,李万军2,刘杰1,3,李晓辉1,*()
ZHANG Chun-Xiao1,LI Shu-Fang1,JIN Feng-Xue1,LIU Wen-Ping1,LI Wan-Jun2,LIU Jie1,3,LI Xiao-Hui1,*()
摘要:
以耐盐碱郑58和盐碱敏感昌7-2为亲本, 构建包含151份F2:5重组自交系(RILs)群体。基于3K芯片对郑58、昌7-2及其F2:5家系进行基因型分析, 构建了包含1407个SNP分子标记的高密度遗传连锁图谱。该图谱的各染色体标记数在84~191之间, 标记间的平均距离为0.81 cM。胁迫液为200 mmol L -1 NaCl和100 mmol L -1 Na2CO3, 对照液为蒸馏水或霍格兰营养液, 对盐、碱胁迫和自然条件下玉米的发芽率(GP)、株高(PH)、植株干、鲜重(FW、DW)、幼苗组织含水量(TWS)、植株地上部分钠含量(SNC)、钾含量(SKC)、钠/钾含量比(NKR)、苗期耐盐率(STR)、耐碱率(ATR) 10项指标, 采用3种不同的作图方法同时定位研究, 对加性QTL定位采用复合区间作图法(CIM)和完备区间作图法(ICIM), 对加性QTL与环境互作联合分析采用混合线性模型的复合区间作图法(MCIM)。结果表明, (1)与对照条件下各性状表型值相比, 耐碱相关性状的降低较耐盐相关性状明显, 说明玉米对碱胁迫更加敏感和碱胁迫对玉米的伤害更严重。碱与盐胁迫下SKC相当而SNC差异较大, 表明Na +、K +的吸收和运输是相互独立的两个过程, 玉米盐、碱胁迫可能是两种性质不同的胁迫。(2)在自然、盐和碱胁迫条件下, 运用CIM分别检测到27、28、40个加性QTL; 运用ICIM分别检测到28、23、17个加性QTL; 运用MCIM共检测到11个耐盐加性QTL、4个环境互作QTL以及11个耐碱加性QTL、3个环境互作QTL。(3)盐胁迫条件下的qPH-9、qSTR-8、qNKR-6、qNKR-7和碱胁迫条件下的qPH-9、qATR-3能被3种作图方法重复检测到。与前人结果比较, qPH-9、qSTR-8、qNKR-6、q-ATR-3定位在相同或邻近区域, qNKR-7尚未见报道。本研究结果为精细定位玉米耐盐碱主效基因、挖掘候选基因和开发用于标记辅助选择的实用功能标记奠定基础。
[1] | 国家统计局. 中国统计年鉴. 北京: 中国统计出版社, 2017. pp 1-12. |
National Bureau of Statistics of the People’s Republic of China. China Statistical Yearbook. Beijing: China Statistical Press, 2017. pp 1-12(in Chinese). | |
[2] | 贾广和 . 盐碱地综合整治与开发研究. 西南林学院学报, 2008,28(4):23-24. |
Jia G H . Study on integrated transformation and development of saline and alkaline and resources. J Southwest For Coll, 2008,28(4):23-24 (in Chinese with English abstract). | |
[3] | 管飞翔 . 玉米RIL芽苗期耐盐碱QTL定位. 扬州大学硕士学位论文, 江苏扬州, 2012. |
Guan F X . Mapping QTL Saline-alkali Tolerance during Bud and Seedling Stage Using RIL in Maize. MS Thesis of Yangzhou University, Jiangsu, Yangzhou,China, 2012 (in Chinese with English abstract). | |
[4] | 王士磊, 高树仁, 王振华, 郎淑平, 王静红 . 玉米重组自交系苗期耐盐相关性状QTL的初步定位. 安徽农业科学, 2012,40:12363-12366. |
Wang S L, Gao S R, Wang Z H, Lang S P, Wang J H . Mapping of QTL associated with salt tolerance in maize inbred line during seedling stage. J Anhui Agric Sci, 2012,40:12363-12366 (in Chinese with English abstract). | |
[5] | 马晓军, 金峰学, 晁青, 张春宵, 杨德光, 李晓辉 . 玉米苗期耐碱性QTL定位. 玉米科学, 2014,22(5):13-19. |
Ma X J, Jin F X, Chao Q, Zhang C X, Yang D G, Li X H . Identification of QTLs for alkaline tolerance at seedling stage in maize. J Maize Sci, 2014,22(5):13-19 (in Chinese with English abstract). | |
[6] | 吴丹丹 . 玉米苗期耐盐性的QTL定位. 山东大学硕士学位论文, 山东济南, 2014. |
Wu D D . QTL Mapping for Salt Tolerance at the Seedling Stage in Maize (Zea mays L.). MS Thesis of Shandong University, Shandong, Jinan,China, 2014 (in Chinese with English abstract). | |
[7] | Mohammad M I H. 玉米耐盐性的鉴定及QTL分析. 中国农业科学院硕士学位论文, 北京, 2013. |
Mohammad M I H . Evaluation and Mapping QTL of Maize Salinity Tolerance. MS Thesis of Chinese Academy of Agricultural Sciences, Beijing,China, 2013 (in Chinese with English abstract). | |
[8] |
Luo M J, Zhao Y X, Zhang R Y, Xing J F, Duan M X, Li J N, Wang N S, Wang W G, Zhang S S, Chen Z H, Zhang H S, Shi Z, Song W, Zhao J R . Mapping of a major QTL for salt tolerance of mature field-grown maize plants based on SNP markers. BMC Plant Biol, 2017,17:140
doi: 10.1186/s12870-017-1090-7 pmid: 5556339 |
[9] | 曲冰冰 . 混合盐碱胁迫对碱地肤生长的影响和统计学分析 . 东北师范大学硕士学位论文, 吉林长春, 2007. |
Qu B B . Effects of Salt-Alkaline Mixed Stresses on Growth of Kochia siecersiana and Statistical Analysis. MS Thesis of Northeast Normal University, Changchun, Jilin, China, 2007 (in Chinese with English abstract). | |
[10] | 河南工业大学. 粮油检验发芽试验: GB/T 5520-2011. 北京: 中国标准出版社, 2011. pp 2-4. |
Henan University of Technology . Inspection of Grain and Oils-germination Test of Seeds: GB/T 5520-2011. Beijing: China Standard Press, 2011. pp 2-4(in Chinese). | |
[11] | 付艳, 高树仁, 王振华 . 玉米种质苗期耐盐性的评价. 玉米科学, 2009,17(1):36-39. |
Fu Y, Gao S R, Wang Z H . Evaluation of salt tolerance of maize germplasm in seedling stage. J Maize Sci, 2009,17(1):36-39 (in Chinese with English abstract). | |
[12] | 王霞, 杨智超, 钱海霞, 高树仁, 孙丽芳 . 添加外源物质硅对NaCl胁迫下玉米幼苗的缓解作用. 安徽农业科学, 2013,41:7404-7405. |
Wang Y, Yang Z C, Qian H X, Gao S R, Sun L F . Relief effect of added exogenous substances Si 4+ under NaCl stress on maize growth . J Anhui Agric Sci, 2013,41:7404-7405 (in Chinese with English abstract). | |
[13] | 刘芳, 付艳, 高树仁, 王振华 . 玉米幼苗的盐胁迫反应及玉米耐盐性的鉴定. 黑龙江八一农垦大学学报, 2007,19(6):22-26. |
Liu F, Fu Y, Gao S R, Wang Z H . Response under salt-stress of maize in seedling stage and appraisal of salt tolerance of maize. J Heilongjiang August First Land Reclamation Univ, 2007,19(6):22-26 (in Chinese with English abstract). | |
[14] | 崔美燕, 高树仁, 付艳, 刘文研, 蔡鑫鑫, 李帅 . 玉米苗期耐碱性鉴定方法研究. 黑龙江八一农垦大学学报, 2008,20(5):12-16. |
Cui M Y, Gao S R, Fu Y, Liu W Y, Cai X X, Li S . Study on appraised methods of alkali tolerance of maize at the stage of seedling. J Heilongjiang August First Land Reclamation Univ, 2008,20(5):12-16 (in Chinese with English abstract). | |
[15] |
Ganal M W, Durstewitz G, Polley A, Berard A, Buckler E S, Charcosset A, Clarke J D, Graner E M, Hansen M, Joets J , LePaslier M C, McMullen M D, Montalent P, Rose M, Schon C C, Sun Q, Walter H, Martin O C, Falque M . A large maize (Zea mays L.) SNP genotyping array: development and germplasm genotyping, and genetic mapping to compare with the B73 reference genome. PLoS One, 2011,6:e28334.
doi: 10.1371/journal.pone.0028334 pmid: 22174790 |
[16] |
Meng L, Li H H, Zhang L Y, Wang J K . QTL IciMapping: Integrated software for genetic linkage map construction and quantitative trait locus mapping in bi-parental populations. Crop J, 2015,3:169-173.
doi: 10.1016/j.cj.2015.01.001 |
[17] |
Kosambi D D . The estimation of map distances from recombination values. Ann Eugenics, 1943,12:172-175.
doi: 10.1111/j.1469-1809.1943.tb02321.x |
[18] | Wang S C, Basten C J, Zeng Z B. Windows QTL Cartographer 2.5. Raleigh, NC, USA: Department of Statistics, North Carolina State University, 2012, Windows QTL Cartographer 2.5. Raleigh, NC, USA: Department of Statistics, North Carolina State University, 2012, . |
[19] |
Yang J, Zhu J, Williams R W . Mapping the genetic architecture of complex traits in experimental populations. Bioinformatics, 2007,23:1527-1536.
doi: 10.1093/bioinformatics/btm143 pmid: 17459962 |
[20] | McCouch S R, Cho Y G, Yano M, Blinstrub M, Morishima H, Kinoshita T . Report on QTL no-menclature. Rice Genet Newsl, 1997,14:11-13. |
[21] | 斯琴巴特尔, 吴红英 . 盐胁迫对玉米种子萌发及幼苗生长的影响. 干旱区资源与环境, 2000,14(4):77-81. |
Sechenbater, Wu H Y . Effect of salt stress on seed germination and seedling growth of Zea mays L. J Arid Land Resour Environ, 2000,14(4):77-81 (in Chinese with English abstract). | |
[22] | 曲元刚, 赵可夫 . NaCl和Na2CO3对玉米生长和生理胁迫效应的比较研究. 作物学报, 2004,30:334-341. |
Qu Y G, Zhao K F . Comparative studies on growth and physiological reaction of Zea mays under NaCl and Na2CO3 stresses. Acta Agron Sin, 2004,30:334-341 (in Chinese with English abstract). | |
[23] | 白文波, 李品芳, 李保国 . NaCl和NaHCO3胁迫下马蔺生长与光合特性的反应. 土壤学报, 2008,45:328-335. |
Bai W B, Li P F, Li B G . Response of Iris lactea var. chinensis to NaCl and NaHCO3 stress in growth and photosynthesis. Acta Pedol Sin, 2008,45:328-335 (in Chinese with English abstract). | |
[24] |
Yang C, Chong J, Li C, Kim C, Shi D, Wang D . Osmotic adjustment and ion balance traits of an alkali resistant halophyte Kochia sieversiana during adaptation to salt and alkali conditions. Plant Soil, 2007,294:263-276.
doi: 10.1007/s11104-007-9251-3 |
[25] | 刘建新, 王鑫, 王瑞娟, 贾海燕 . 黑麦草对NaHCO3胁迫的光合生理响应. 草业学报, 2012,21(3):184-190. |
Liu J X, Wang X, Wang R J, Jia H Y . Photosynthetic physiological response of Lolium prenne to NaHCO3 stress. Acta Pratac Sin, 2012,21(3):184-190 (in Chinese with English abstract). | |
[26] | 邸宏, 刘学 . 13份玉米自交系的耐碱性评价. 贵州农业科学, 2016,44(2):15-19. |
Di H, Liu X . Alkali tolerance of thirteen inbred lines in maize. Guizhou Agric Sci, 2016,44(2):15-19 (in Chinese with English abstract). | |
[27] |
Wang Z F, Chen Z W, Cheng J P, Lai Y Y, Wang J F, Bao Y M, Huang J, Zhang H S . QTL analysis of Na+ and K+ concentrations in roots and shoots under different levels of NaCl stress in rice ( Oryza sativa L.). PLoS One, 2012,7:e51202.
doi: 10.1371/journal.pone.0051202 pmid: 23236455 |
[28] |
Cheng L R, Wang Y, Meng L J, Hu X, Cui Y R, Sun Y, Zhu L H, Ali J, Xu J L, Li J K . Identification of salt-tolerant QTLs with strong genetic background effect using two sets of reciprocal introgression lines in rice. Genome, 2012,55:45-55.
doi: 10.1139/g11-075 pmid: 22181322 |
[29] |
Lee G J, Boerma H R, Villagarcia M R, Zhou X , Carter T E Jr, Li Z, Gibbs M O. A major QTL conditioning salt tolerance in S-100 soybean and descendent cultivars. Theor Appl Genet, 2004,109:1610-1619.
doi: 10.1007/s00122-004-1783-9 pmid: 15365627 |
[30] | 梁银培, 孙健, 索艺宁, 刘化龙, 王敬国, 郑洪亮, 孙晓雪, 邹德堂 . 水稻耐盐性和耐碱性相关性状的QTL定位及环境互作分析. 中国农业科学, 2017,50:1747-1762. |
Liang Y P, Sun J, Suo Y N, Liu H L, Wang J G, Zheng H, Sun X X, Zou D T . QTL mapping and QTL × environment interaction analysis of salt and alkali tolerance-related traits in rice ( Oryza sativa L.). Sci Agric Sin, 2017,50:1747-1762 (in Chinese with English abstract). | |
[31] | 梁慧珍, 余永亮, 杨红旗, 张海洋, 董薇, 崔暐文, 杜华, 刘学义, 方宣钧 . 大豆小区产量及其相关性状QTL间的上位性和环境互作效应. 植物学报, 2014,49:273-281. |
Liang H Z, Yu Q L, Yang H Q, Zhang H Y, Dong W, Cui W W, Du H, Liu Y, Fang X J . Epistatic effects and quantitative trait loci (QTL) × environment (QE) interaction effects for yield per plot and botanical traits in soybean. Chin Bull Bot, 2014,49:273-281 (in Chinese with English abstract). | |
[32] | 苏成付, 赵团结, 盖钧镒 . 不同统计遗传模型QTL定位方法应用效果的模拟比较. 作物学报, 2010,36:1100-1107. |
Su C F, Zhao T J, Gai J Y . Simulation comparisons of effectiveness among QTL mapping procedures of different statistical genetic models. Acta Agron Sin, 2010,36:1100-1107 (in Chinese with English abstract). | |
[33] | Zhang W J, Niu Y, Bu S H, Li M, Feng J Y, Zhang J, Yang S X, Odinga M M, Wei S P, Liu X F, Zhang Y M . Epistatic association mapping for alkaline and salinity tolerance traits in the soybean germination stage. PLoS One, 2014,9:e84750. |
[34] |
Cui D Z, Wu D D, Somarathna Y, Xu C Y, Li S, Li P, Zhang H, Chen H B, Zhao L . QTL mapping for salt tolerance based on SNP markers at the seedling stage in maize (Zea mays L.). Euphytica, 2015,203:273-283.
doi: 10.1007/s10681-014-1250-x |
[35] | Bizimana J B, Luzi-Kihupi A, Murori R W, Singh R K . Identification of quantitative trait loci for salinity tolerance in rice ( Oryza sativa L.) using IR29/Hasawi mapping population. J Genet, 2017,96:571-582. |
[36] | 赵振卿, 顾宏辉, 盛小光, 虞慧芳, 王建升, 曹家树 . 作物数量性状位点研究进展及其育种应用. 核农学报, 2014,28:1615-1624. |
Zhao Z Q, Gu H H, Sheng X G, Yu H F, Wang J S, Cao J S . Advances and applications in crop quantitative trait locus. J Nucl Agric Sci, 2014,28:1615-1624 (in Chinese with English abstract). |
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