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作物学报 ›› 2019, Vol. 45 ›› Issue (11): 1735-1745.doi: 10.3724/SP.J.1006.2019.94022

• 耕作栽培·生理生化 • 上一篇    下一篇

基于多重表型分析的准确评价高粱抗旱性方法的建立

张笑笑1,2,潘映红3,任富莉2,蒲伟军2,王道平3,李玉斌2,陆平3,李桂英3,*(),朱莉2,*()   

  1. 1 西南科技大学, 四川绵阳 621000
    2 中国农业科学院生物技术研究所, 北京 100081
    3 中国农业科学院作物科学研究所, 北京 100081
  • 收稿日期:2019-02-14 接受日期:2019-05-12 出版日期:2019-11-12 网络出版日期:2019-06-04
  • 通讯作者: 李桂英,朱莉
  • 作者简介:E-mail: 1826437817@qq.com
  • 基金资助:
    本研究由中央级公益性科研院所基本科研业务费专项(Y2017PT25);国家自然科学基金项目(31471558);国家重点研发项目(2018YFD1000702);国际原子能机构协调研究项目(D23031)

Establishment of an accurate evaluation method for drought resistance based on multilevel phenotype analysis in sorghum

ZHANG Xiao-Xiao1,2,PAN Ying-Hong3,REN Fu-Li2,PU Wei-Jun2,WANG Dao-Ping3,LI Yu-Bin2,LU Ping3,LI Gui-Ying3,*(),ZHU Li2,*()   

  1. 1 Southwest University of Science and Technology, Mianyang 621000, Sichuan, China
    2 Institute of Biotechnology, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    3 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2019-02-14 Accepted:2019-05-12 Published:2019-11-12 Published online:2019-06-04
  • Contact: Gui-Ying LI,Li ZHU
  • Supported by:
    This study was supported by the Fundamental Research Funds for Central Non-profit Scientific Institution(Y2017PT25);the National Natural Science Foundation of China(31471558);the National Key R&D Program of China(2018YFD1000702);the IAEA Coordinated Research Project(D23031)

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摘要：

干旱是制约农业生产的重要非生物胁迫之一, 因此作物抗旱性研究具有重要理论和应用价值。本研究对73份高粱材料萌发期和苗期的抗旱性进行了初筛, 从中选择15份抗旱材料和6份敏感材料, 通过室内和田间试验, 获得干旱胁迫下幼苗的株高、叶长、叶宽等形态特征以及超氧化物歧化酶、丙二醛、过氧化物酶等生理生化指标的响应数据, 并对高粱的抗旱性做进一步的验证, 最终确定抗旱型材料1份, 中等抗旱型材料1份, 干旱敏感型材料2份。本研究建立了一套基于多重表型分析的高粱抗旱性研究新方法, 即综合运用多种数据分析方法(包括隶属函数法、主成分分析法、聚类分析法), 结合不同生长环境(田间试验和室内试验)和不同生育期(萌发期和苗期)高粱响应干旱胁迫的多种表型数据(形态特征、生理生化指标), 通过多重比较高粱抗旱性评价结果的一致性, 旨在系统、高效、准确地判定高粱的抗旱性, 为高粱抗旱机制的研究和抗旱新品种的选育提供技术支撑。

关键词: 多重表型分析, 高粱, 抗旱性, 萌发期, 苗期

Abstract:

Drought is one of the important abiotic stresses that restrict agricultural production. Research on drought resistance of crops is of significance in both theoretical and practical aspects. In this study, 73 sorghum accessions were preliminarily evaluated for drought resistance at the germination and seedling stages under simulated drought environment with PEG-6000. Among them, 21 accessions including 15 tolerant and 6 susceptible, were screened to measure the morphological characteristics such as plant height, leaf length and leaf width, and physiological and biochemical indicators such as superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, and peroxidase (POD) activity. Finally, one accession was identified with high drought resistance, one accession was moderate drought resistance and two accessions with drought-sensitivity. A new evaluation method for sorghum drought resistance was established based on the multilevel phenotypic analysis, in which the data from subordinate function method, principal component analysis and clustering analysis were comprehensively applied, and combined with various phenotypic data (morphological, physiological and biochemical indicators) at different growth stages (germination and seedling stages) in different growth environments (field test and laboratory test). This method can be used to systematically, efficiently and accurately identify the drought resistance of sorghum based on the consistency of evaluation results by multiple comparison analysis, which is useful in the research on drought resistance mechanism and sorghum breeding for drought resistance.

Key words: multilevel phenotypic analysis, sorghum, drought resistance, germination stage, seedling stage

图1

多重表型分析方法概要"

表1

73份高粱材料信息"

编号
Number		 名称
Name		 编号
Number		 名称
Name
D01 Club.S D38 SEM-20-4
D02 IS-8377 D39 SEM-20-5
D03 5933 D40 SEM-28-4
D04 M81-E D41 SEM-30-1
D05 SEM-2-4 D42 SEM-31-4
D06 灯笼红(忻州) Denglonghong (Xinzhou) D43 SEM-32-1
D07 黑暴糯高粱(广南) Heibaonuogaoliang (Guangnan) D44 SEM-32-2
D08 Bird proof kafer #-662 D45 SEM-32-4
D09 矮脚糯(平利) Aijiaonuo (Pingli) D46 SEM-32-5
D10 糯高粱(宁夏) Nuogaoliang (Ningxia) D47 SEM-32-9
D11 糯高粱(柞水) Nuogaoliang (Zuoshui) D48 SEM-32-4
D12 CLUBHEAD-3 D49 SEM-32-5
D13 甜131 Tian 131 D50 SEM-32-9
编号
Number		 名称
Name		 编号
Number		 名称
Name
D14 千斤锤(沁水) Qianjinchui (Qinshui) D51 SEM-38-4
D15 甜选29 Tianxuan 29 D52 SEM-38-7
D16 甜选37 Tianxuan 37 D53 SEM-42-1
D17 甜选129 Tianxuan 129 D54 SEM-44-2
D18 Icsv 219 D55 SEM-54-1
D19 木浦在来种Mupuzailaizhong D56 SEM-54-5
D20 IS-9180 D57 SEM-21-8
D21 10048 D58 SEM-24-6
D22 AIRI-2 D59 SEM-24-7
D23 东北农信糯Dongbeinongxinnuo D60 SEM-30-3
D24 河北糯杂5号Heibeinuoza 5 D61 SEM-49-2
D25 河北糯杂6号Hebeinuoza 6 D62 SEM-49-4
D26 红茅糯2号Hongmaonuo 2 D63 SEM-51-11
D27 山东莱阳糯Shandonglaiyangnuo D64 SEM-53-4
D28 原15B Yuan 15B D65 SEM-53-5
D29 耘糯Yunnuo D66 SEM-53-7
D30 上海甜Shanghaitian D67 SEM-53-10
D31 软高粱(定襄) Ruangaoliang (Dingxiang) D68 SEM-57-4
D32 米高粱(清涧) Migaoliang (Qingjian) D69 SEM-56-8
D33 CP16-1481 D70 SEM-62-5
D34 SEM-8-4 D71 SEM-66-2
D35 SEM-16-10 D72 科甜5号Ketian 5
D36 SEM-17-3 D73 SEM-42-4
D37 SEM-20-2

图2

不同高粱材料萌发期的抗旱指标隶属函数值及苗期萎蔫指数等级 RGP: 相对发芽势; RGR: 相对发芽率; DGRI: 萌发抗旱指数; ASFV: 平均隶属函数值; 1-WI: 1-苗期萎蔫指数等级赋值。"

表2

21份高粱材料基于隶属函数法的苗期抗旱性综合评价"

材料编号
Accession number		 田间抗旱性排序
Field drought
resistance ranking		 室内抗旱性排序
Laboratory drought resistance ranking		 材料编号
Accession number		 田间抗旱性排序
Field drought
resistance ranking		 室内抗旱性排序
Laboratory drought resistance ranking
D11 1 1 D51 11 16
D16 2 3 D40 13 5
D05 3 15 D04 14 18
D37 4 4 D62 15 6
D41 5 19 D24 16 14
D43 6 17 D60 17 11
D18 7 8 D42 18 10
D19 8 7 D22 19 2
D49 9 20 D53 20 21
D14 10 9 D65 21 13
D10 11 12

图3

21份高粱材料基于主成分分析法的苗期抗旱性综合评价 A: 21份高粱材料田间抗旱性综合得分; B: 21份高粱材料室内抗旱性综合得分。"

图4

21份高粱材料基于聚类分析法的苗期抗旱性评价 A: 21份高粱材料田间抗旱性聚类分析结果; B: 21份高粱材料室内抗旱性聚类分析结果。"

图5

不同高粱抗旱性多重表型分析结果的差异度比较 A: 不同分析方法和不同生长环境的多重表型分析结果的差异度比较; B: 不同生长环境下高粱抗旱性多重表型分析结果的差异度比较。a: 田间试验条件下隶属函数法与主成分分析法分析结果之间的比较; b: 室内试验条件下隶属函数法与主成分分析法结果之间比较; c: 田间与室内试验主成分分析法结果比较; d: 田间与室内试验隶属函数法结果比较; e: 田间试验隶属函数法与室内试验主成分分析法结果之间比较。其中实线(0.5和1.0)作为c和d差异值的参考线, 用以评估不同生长环境同一分析方法多重表型分析结果的差异度; 虚线(1.20和1.75)和实线(1.5)作为e差异值的参考线, 用以评估田间试验隶属函数与室内试验主成分分析结果的差异度。"

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