作物学报 ›› 2019, Vol. 45 ›› Issue (11): 1735-1745.doi: 10.3724/SP.J.1006.2019.94022
张笑笑1,2,潘映红3,任富莉2,蒲伟军2,王道平3,李玉斌2,陆平3,李桂英3,*(),朱莉2,*()
ZHANG Xiao-Xiao1,2,PAN Ying-Hong3,REN Fu-Li2,PU Wei-Jun2,WANG Dao-Ping3,LI Yu-Bin2,LU Ping3,LI Gui-Ying3,*(),ZHU Li2,*()
摘要:
干旱是制约农业生产的重要非生物胁迫之一, 因此作物抗旱性研究具有重要理论和应用价值。本研究对73份高粱材料萌发期和苗期的抗旱性进行了初筛, 从中选择15份抗旱材料和6份敏感材料, 通过室内和田间试验, 获得干旱胁迫下幼苗的株高、叶长、叶宽等形态特征以及超氧化物歧化酶、丙二醛、过氧化物酶等生理生化指标的响应数据, 并对高粱的抗旱性做进一步的验证, 最终确定抗旱型材料1份, 中等抗旱型材料1份, 干旱敏感型材料2份。本研究建立了一套基于多重表型分析的高粱抗旱性研究新方法, 即综合运用多种数据分析方法(包括隶属函数法、主成分分析法、聚类分析法), 结合不同生长环境(田间试验和室内试验)和不同生育期(萌发期和苗期)高粱响应干旱胁迫的多种表型数据(形态特征、生理生化指标), 通过多重比较高粱抗旱性评价结果的一致性, 旨在系统、高效、准确地判定高粱的抗旱性, 为高粱抗旱机制的研究和抗旱新品种的选育提供技术支撑。
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