作物学报 ›› 2020, Vol. 46 ›› Issue (10): 1639-1646.doi: 10.3724/SP.J.1006.2020.04056
• 研究简报 • 上一篇
胡茂龙1,2(), 程丽1, 郭月1, 龙卫华1, 高建芹1, 浦惠明1,*(), 张洁夫1,2, 陈松1
HU Mao-Long1,2(), CHENG Li1, GUO Yue1, LONG Wei-Hua1, GAO Jian-Qin1, PU Hui-Ming1,*(), ZHANG Jie-Fu1,2, CHEN Song1
摘要:
草害已成为严重制约我国油菜生产的重要因素。种植抗除草剂品种和采用化学除草是防控草害的经济有效途径。为了开展分子标记辅助选择(marker-assisted selection, MAS)育种, 加速抗除草剂品种培育, 本研究针对已发现的抗咪唑啉酮类除草剂油菜M9中BnALS1R基因编码区第1913位点的SNP变异, 开发高通量、低成本的竞争性等位基因特异PCR (kompetitive allele specific PCR, KASP)标记。采用筛选出的KBA1R19681913B标记在2个F2群体中进行KASP反应。结果表明, 该标记能有效检测群体中存在的BnALS1R 3种基因型, 其分离比均为1︰2︰1, 遵循单基因遗传规律, 且基因型与表型完全吻合。将该标记用于BnALS1R抗性纯合基因的回交转育, 获得200多个抗咪唑啉酮油菜恢复系。该标记还可在油菜苗期鉴定抗性杂交种的纯度。KASP标记KBA1R19681913B的获得为油菜抗除草剂MAS育种以及抗性新种质的培育提供了技术支撑。
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