不同施肥水平下丛枝菌根真菌对甘蔗生长及养分相关基因共表达网络的影响

doi:10.3724/SP.J.1006.2022.14052

作物学报 ›› 2022, Vol. 48 ›› Issue (4): 860-872.doi: 10.3724/SP.J.1006.2022.14052

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

不同施肥水平下丛枝菌根真菌对甘蔗生长及养分相关基因共表达网络的影响

孔垂豹¹(), 庞孜钦¹^,², 张才芳¹, 刘强¹^,², 胡朝华¹, 肖以杰¹, 袁照年¹^,³^,^*()

¹福建农林大学国家甘蔗工程技术研究中心, 福建福州 350002
²福建农林大学农学院, 福建福州 350002
³蔗糖产业省部共建协同创新中心, 广西南宁 530000

收稿日期:2021-03-31 接受日期:2021-07-12 出版日期:2022-04-12 网络出版日期:2021-07-26
通讯作者: 袁照年
作者简介:E-mail: kongchuibao18@163.com
基金资助:
国家现代农业产业技术体系建设专项(CARS-170208);福建农林大学科技创新项目资助(KFA17528A)

Effects of arbuscular mycorrhizal fungi on sugarcane growth and nutrient- related gene co-expression network under different fertilization levels

KONG Chui-Bao¹(), PANG Zi-Qin¹^,², ZHANG Cai-Fang¹, LIU Qiang¹^,², HU Chao-Hua¹, XIAO Yi-Jie¹, YUAN Zhao-Nian¹^,³^,^*()

¹National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
²College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
³Province and Ministry Co-sponsored Collaborative Innovation Center of Sugar Industry, Nanning 530000, Guangxi, China

Received:2021-03-31 Accepted:2021-07-12 Published:2022-04-12 Published online:2021-07-26
Contact: YUAN Zhao-Nian
Supported by:
China Agriculture Research System(CARS-170208);Science and Technology Innovation Project of Fujian Agriculture and Forestry University(KFA17528A)

摘要/Abstract

摘要：

甘蔗是我国重要的糖料作物之一。丛枝菌根(arbuscular mycorrhizas, AM)真菌在自然界中分布广泛, 研究表明AM真菌侵染植物根系能够促进植物的养分吸收和生长发育。本研究利用盆栽试验设置常规施肥(N)和减量施肥(R) 2个施肥水平, 并分别进行接种(AM)和未接种(CK) AM真菌处理, 共计4组, 每组设置4个重复。结果表明, 接种AM真菌不仅可以显著提高甘蔗地上及地下部生物量的积累, 而且还显著影响甘蔗根际土壤pH值、碱解氮、有效磷的含量。同时, 接种AM真菌在减量施肥水平下对甘蔗生物量的提高作用明显高于常规施肥水平。通过加权基因共表达网络分析(weighted gene co-expression network analysis, WGCNA)筛选到与氮、磷等养分表型存在高度特异性的turquoise模块和darkgreen模块。以KME值大于0.7为阈值筛选模块的核心基因, 分别筛选到408个和21个核心基因。通过GO富集分析发现, 这些核心基因主要参与养分转运、代谢以及催化活性等通路。根据基因的注释信息以及基因间的连通性, 在筛选出的核心基因中寻找到28个与氮、磷等养分吸收与转运相关的核心基因以及108个候选基因。本研究揭示了AM真菌对甘蔗养分吸收的影响效应, 为进一步理解AM真菌影响甘蔗养分吸收的分子机制提供了理论基础。

关键词: 丛枝菌根真菌, 甘蔗生长, 养分, 加权基因共表达网络

Abstract:

Sugarcane is one of the important sugar crops in China. Arbuscular mycorrhizas (AM) fungi are widely distributed. Researches have shown that AM fungi infecting plant roots can promote nutrient absorption and growth of plants. In this study, pot experiment was used to set up with two fertilization levels of conventional fertilization (N) and reduced fertilization (R), and inoculated (AM) and control (CK). There were four treatments in total, and four replicates were set in each treatment. The results revealed that the plants inoculated with AM fungi did not only significantly increased the biomass accumulation, but also significantly affected the pH value, alkali hydrolyzable nitrogen and available phosphorus of sugarcane rhizosphere soil. The biomass accumulation in the AM fungus inoculation treatment under reduced fertilization was significantly higher than that of conventional fertilization. The turquoise module and darkgreen module with high specificity with nutrient phenotypes such as nitrogen and phosphorus were screened by weighted gene co-expression network analysis (WGCNA). The core genes of the module were screened with KME value greater than 0.7 as the threshold, and 408 and 21 core genes were screened, respectively. GO enrichment indicated that these core genes were mainly involved in nutrient transport, metabolism, and enzyme catalysis pathways. Based on annotation information and the connectivity of genes, 28 core genes related to the absorption and transportation of nutrients such as nitrogen and phosphorus and 108 related candidate genes were detected among the core genes screened. This study reveals the effects of AM fungi on sugarcane nutrient absorption, and provides a theoretical basis for further understanding of the molecular mechanisms of AM fungi affecting sugarcane nutrient absorption.

Key words: arbuscular mycorrhizal fungi, sugarcane growth, nutrients, WGCNA

孔垂豹, 庞孜钦, 张才芳, 刘强, 胡朝华, 肖以杰, 袁照年. 不同施肥水平下丛枝菌根真菌对甘蔗生长及养分相关基因共表达网络的影响[J]. 作物学报, 2022, 48(4): 860-872.

KONG Chui-Bao, PANG Zi-Qin, ZHANG Cai-Fang, LIU Qiang, HU Chao-Hua, XIAO Yi-Jie, YUAN Zhao-Nian. Effects of arbuscular mycorrhizal fungi on sugarcane growth and nutrient- related gene co-expression network under different fertilization levels[J]. Acta Agronomica Sinica, 2022, 48(4): 860-872.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2022.14052

https://zwxb.chinacrops.org/CN/Y2022/V48/I4/860

图/表 10

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表3

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表5

各基因模块中养分相关核心基因功能注释(部分)"

模块 Module	基因ID Gene ID	基因功能 Gene function
碧绿	Saccharum_officinarum_newGene_73305	蔗糖合酶2
Turquoise		Sucrose synthase 2
	Saccharum_officinarum_newGene_56251	氨基酸转运和代谢
		Amino acid transport and metabolism
	Saccharum_officinarum_newGene_107291	氨基酸转运和代谢
		Amino acid transport and metabolism
	Saccharum_officinarum_newGene_109658	可溶性无机焦磷酸酶; 无机离子迁移与代谢
		Soluble inorganic pyrophosphatase; inorganic ion transport and metabolism
	Saccharum_officinarum_newGene_84225	碳水化合物的运输和代谢
		Carbohydrate transport and metabolism
	Saccharum_officinarum_newGene_20999	氨基酸转运和代谢
		Amino acid transport and metabolism
	Saccharum_officinarum_newGene_56328	碳水化合物的运输和代谢
		Carbohydrate transport and metabolism
模块 Module	基因ID Gene ID	基因功能 Gene function
	Saccharum_officinarum_newGene_61584	氨基酸转运和代谢
		Amino acid transport and metabolism
	Saccharum_officinarum_newGene_20996	氨基酸转运和代谢
		Amino acid transport and metabolism
	Saccharum_officinarum_newGene_36202	钾离子通道基因CNGC2
		Cyclic nucleotide-gated ion channel 2
	Saccharum_officinarum_newGene_101569	可溶性无机焦磷酸酶
		Soluble inorganic pyrophosphatase
	Saccharum_officinarum_newGene_36232	钾离子通道基因CNGC2
		Cyclic nucleotide-gated ion channel 2
	Saccharum_officinarum_newGene_60132	碳水化合物的运输和代谢
		Carbohydrate transport and metabolism
	Saccharum_officinarum_newGene_116537	主要诱导超级家族; 糖(及其他)转运蛋白
		Major Facilitator Super family; sugar (and other) transporter
	Saccharum_officinarum_newGene_68404	淀粉和蔗糖代谢; 氨基糖和核苷酸糖代谢
		Starch and sucrose metabolism; amino sugar and nucleotide sugar metabolism
	Saccharum_officinarum_newGene_52856	碳水化合物的运输和代谢
		Carbohydrate transport and metabolism
	Saccharum_officinarum_newGene_61188	主要诱导超级家族; 糖(及其他)转运蛋白
		Major Facilitator Super family; sugar (and other) transporter
	Saccharum_officinarum_newGene_60391	氨基酸转运和代谢
		Amino acid transport and metabolism
	Saccharum_officinarum_newGene_54480	主要诱导超级家族; 糖(及其他)转运蛋白
		Major Facilitator Super family; sugar (and other) transporter
	Saccharum_officinarum_newGene_116260	NRT1/PTR FAMILY 7.2蛋白基因
		Protein NRT1/PTR FAMILY 7.2
	Saccharum_officinarum_newGene_52960	氨基酸转运和代谢
		Amino acid transport and metabolism
	Saccharum_officinarum_newGene_76759	多肌醇多磷酸磷酸酶1
		Multiple inositol polyphosphate phosphatase 1
	Saccharum_officinarum_newGene_113779	NRT1/PTR FAMILY 6.4蛋白基因
		Protein NRT1/PTR FAMILY 6.4
	Saccharum_officinarum_newGene_1327	NRT1/PTR FAMILY 6.4蛋白基因
		Protein NRT1/PTR FAMILY 6.4
深绿	Saccharum_officinarum_newGene_108443	碳水化合物的运输和代谢
Darkgreen		Carbohydrate transport and metabolism
	Saccharum_officinarum_newGene_105049	磷酸酯酶家族基因
		Phosphoesterase family
	Saccharum_officinarum_newGene_48314	可溶性无机焦磷酸酶; 能源生产与转化
		Soluble inorganic pyrophosphatase; energy production and conversion
	Saccharum_officinarum_newGene_90724	碳水化合物的运输和代谢
		Carbohydrate transport and metabolism

表5

图5

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处理 Treatment	植株干重 Plant biomass (g)	株高 Plant height (cm)	菌丝侵染率 Mycelial infection rate (%)
AMN	31.00±0.20 a	41.43±3.27 a	31.25±4.50 b
AMR	27.47±0.84 b	40.09±4.87 a	50.25±8.14 a
CKN	26.37±0.59 b	29.93±0.92 b	0.25±0.25 c
CKR	21.24±0.24 c	23.46±1.13 b	0.50±0.29 c

处理 Treatment	根干重 Root biomass (g)	根长 Length (cm)	根表面积 Surface area (cm²)
AMN	2.85±0.12 ab	15055.92±162.27 b	1636.08±54.34 a
AMR	3.13±0.06 a	17668.88±220.10 a	1676.34±20.91 a
CKN	2.56±0.02 b	12742.93±158.71 c	1258.49±65.41 c
CKR	2.71±0.20 b	13360.82±365.33 c	1408.73±23.45 b

处理 Treatment	酸碱度 pH	碱解氮 AN (mg kg^-1)	有效磷 AP (mg kg^-1)	速效钾 AK (mg kg^-1)	有机质 OM (g kg^-1)
AMN	6.75±0.02 b	42.47±2.52 a	12.84±0.25 a	27.38±1.28 a	9.82±0.25 a
AMR	6.92±0.03 a	36.95±3.48 a	10.86±0.37 b	25.63±0.13 ab	7.28±0.14 b
CKN	6.68±0.02 c	26.99±1.52 b	8.89±0.50 c	25.13±1.01 ab	6.79±0.22 b
CKR	6.73±0.02 bc	24.66±1.19 b	7.55±0.16 d	24.00±0.35 b	4.14±0.02 c

模块 Module	GO富集项目 GO term	GO	基因数目 Number of genes	P值 P-value
碧绿Turquoise	代谢过程Metabolic process	GO:0008152	122	2.40E-04
	细胞过程Cellular process	GO:0009987	119	4.70E-03
	单一生物过程Single-organism process	GO:0044699	99	4.13E-03
	生物调节Biological regulation	GO:0065007	37	4.27E-03
	对刺激的反应Response to stimulus	GO:0050896	35	4.78E-03
	本质化过程Localization	GO:0051179	29	4.74E-03
	细胞Cell	GO:0005623	133	5.11E-04
	细胞部分Cell part	GO:0044464	133	2.39E-03
	细胞器Organelle	GO:0043226	100	7.90E-05
	膜Membrane	GO:0016020	92	2.04E-03
	膜部分Membrane part	GO:0044425	75	2.52E-03
	细胞器部分Organelle part	GO:0044422	29	3.51E-03
	结合Binding	GO:0005488	108	2.58E-03
	催化活性Catalytic activity	GO:0003824	98	7.54E-03
深绿Darkgreen	代谢过程Metabolic process	GO:0008152	13	1.25E-03
	细胞过程Cellular process	GO:0009987	8	3.45E-03
	单一生物过程Single-organism process	GO:0044699	8	2.54E-03
	膜Membrane	GO:0016020	8	3.25E-03
	细胞Cell	GO:0005623	7	6.45E-03
	细胞部分Cell part	GO:0044464	7	5.87E-03
	膜部分Membrane part	GO:0044425	7	2.58E-03
	细胞器Organelle	GO:0043226	6	4.65E-03
	催化活性Catalytic activity	GO:0003824	11	2.35E-03
	结合Binding	GO:0005488	8	4.78E-03

不同施肥水平下丛枝菌根真菌对甘蔗生长及养分相关基因共表达网络的影响

Effects of arbuscular mycorrhizal fungi on sugarcane growth and nutrient- related gene co-expression network under different fertilization levels

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