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作物学报 ›› 2022, Vol. 48 ›› Issue (11): 2683-2690.doi: 10.3724/SP.J.1006.2022.21017

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

矮败小麦技术体系在黄淮冬麦区南片抗赤霉病育种中的应用

周阳1,*(), 张宏军1, 王晨阳2, 李洪杰1, 买春艳3, 杨丽1, 刘宏伟1, 于立强4, 于广军4, 刘秉华1   

  1. 1中国农业科学院作物科学研究所 / 作物分子育种国家工程实验室, 北京100081
    2河南农业大学 / 国家小麦工程技术研究中心, 河南郑州450002
    3新乡矮败小麦育种技术创新中心, 河南新乡453700
    4石家庄市农林科学研究院赵县实验基地, 河北赵县051530
  • 收稿日期:2022-01-27 接受日期:2022-04-30 出版日期:2022-11-12 网络出版日期:2022-05-07
  • 通讯作者: 周阳
  • 基金资助:
    本研究由国家重点研发计划项目(2016YFD0101802);中国农业科学院创新工程项目资助。

Application of dwarf-male-sterile wheat in breeding program for Fusarium head blight resistance in Southern Yellow-Huai Rivers Valley Winter Wheat Region

ZHOU Yang1,*(), ZHANG Hong-Jun1, WANG Chen-Yang2, LI Hong-Jie1, MAI Chun-Yan3, YANG Li1, LIU Hong-Wei1, YU Li-Qiang4, YU Guang-Jun4, LIU Bing-Hua1   

  1. 1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Engineering Research Center of Crop Molecular Breeding, Beijing 100081, China
    2Henan Agricultural University / National Wheat Engineering Research Center, Zhengzhou 450002, Henan, China
    3Xinxiang Innovation Center for Breeding Technology of Dwarf-Male-Sterile Wheat, Xinxiang 453731, Henan, China
    4Zhaoxian Experiment Station, Shijiazhuang Academy of Agricultural and Forestry Sciences, Zhaoxian 051530, Hebei, China
  • Received:2022-01-27 Accepted:2022-04-30 Published:2022-11-12 Published online:2022-05-07
  • Contact: ZHOU Yang
  • Supported by:
    The National Key Research and Development Program of China(2016YFD0101802);The Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences.

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摘要：

矮败小麦是一个便利的杂交育种工具材料。我们在育种实践中不断对矮败小麦技术体系进行改进, 改进后的矮败小麦技术体系包括创制矮败小麦骨干亲本、围绕骨干亲本构建大规模有限回交育种群体、分子标记辅助选择、单倍体育种应用和异地加代技术几项主要内容。利用该技术体系, 以黄淮冬麦区高产抗赤霉病为主要育种目标进行了育种实践, 成功将抗赤霉病基因Fhb1快速导入黄淮冬麦区小麦品种遗传背景, 比常规育种方法提前2~3年育成了高产、中抗赤霉病、适宜黄淮冬麦区种植的小麦新品系, 显著提高了高产抗赤霉病育种效率。

关键词: 矮败小麦, 抗赤霉病育种, Fhb1, 分子标记

Abstract:

The dwarf-male-sterile (DMS) wheat has been used as a convenient cross breeding tool. We have upgraded DMS wheat breeding system, which included as follows: (1) development of a series of DMS wheat parental near-isogenic lines by backcrossing the DMS wheat with elite wheat parental varieties; (2) establishment of a large-scale backcrossing population by crossing the DMS wheat parental near-isogenic lines to the cultivars (lines) with the traits of interests in the breeding program; (3) application of marker-assisted selection for the major target genes; (4) induction of DMS double-haploid (DH) lines using maize pollen pollination; and (5) approach of shuttle breeding for off-season traits evaluation. By applying this system, we successfully introduced the Fusarium head blight (FHB) resistance gene Fhb1 into the genetic background of elite wheat varieties in Yellow-Huai River Valley Winter Wheat Region of China. A number of high-yielding and FHB resistance wheat lines were developed with 2-3 years in advance comparing with conventional breeding method. This method significantly increased the breeding efficiency and has a great potential for wheat breeding program.

Key words: dwarf-male-sterile wheat, Fusarium head blight, Fhb1, molecular breeding

图1

矮败小麦在黄淮冬麦区南片抗赤霉病育种中应用的技术体系 DMS: 矮败小麦; NIL: 近等基因系; FHB: 赤霉病。"

表1

2个(轮选136和轮选13)背景下Fhb1不同基因型间主要农艺性状比较"

年份
Year		 基因型
Genotype		 株高
Plant
height (cm)		 单株穗数
Spike number
per plant		 穗长
Spike length (cm)		 每穗小穗数
Spikelet number
per spike		 不育小穗数
Sterile spikelet
per spike		 单穗粒重
Kernel weight per spike (g)		 每穗粒数
Kernel number
per spike		 千粒重
1000-kernel weight (g)
2017 轮选136背景Lunxuan 136 background
Fhb1-H 77.2 a 24.1 a 10.6 a 22.1 a 1.5 a 2.8a 49.1 a 56.9 a
Fhb1-S 75.3 b 24.1 a 10.7 a 22.3 a 1.5 a 2.8 a 50.1 a 56.7 a
轮选13背景Lunxuan 13 background
Fhb1-H 76.5 a 22.9 a 9.7 a 22.8 a 1.4 a 2.9 a 55.0 a 53.3 a
Fhb1-S 77.2 a 22.6 a 9.8 a 23.1 b 1.4 a 2.9 a 54.8 a 53.0 a
2018 轮选136背景 Lunxuan 136 background
Fhb1-H 63.4 b 9.7 a 7.8 a 19.3 a 1.3 a 2.1 a 40.5 a 40.5 a
Fhb1-R 63.7 ab 9.4 ab 7.7 a 19.4 a 1.3 a 2.0 a 39.3 a 39.3 a
Fhb1-S 64.4 a 9.2 b 7.8 a 19.6 a 1.2 a 2.1 a 40.0 a 40.0 a
轮选13背景 Lunxuan 13 background
Fhb1-H 62.4 a 9.0 a 7.5 a 20.3 a 1.0 a 2.2 a 46.6 a 46.6 a
Fhb1-R 62.9 a 9.2 a 7.5 a 20.4 a 1.0 a 2.2 a 47.4 a 46.3 a
Fhb1-S 62.4 a 9.1 a 7.5 a 20.5 a 1.0 a 2.2 a 47.6 a 46.9 a
[1] Gromadzka K, Waskiewicz A, Chelkowski J, Golinski P. Zearalenone and its metabolites: occurrence, detection, toxicity and guidelines. World Mycotoxin J, 2008, 1: 209-220.
doi: 10.3920/WMJ2008.x015
[2] Zinedine E A, Soriano J M, Moito J C, Manes J. Review on the toxicity, occurrence, metabolism, detoxification, regulations and intake of zearalenone: an oestrogenic mycotoxin. Food Chem Toxicol, 2007, 45: 1-18.
doi: 10.1016/j.fct.2006.07.030
[3] 商鸿生, 王树权, 井金学. 关中灌区小麦赤霉病流行因素分析. 中国农业科学, 1987, 20(5): 71-75.
Shang H S, Wang S Q, Jing J X. Analysis of factors in epidemic of Fusarium head blight in Guanzhong irrigated area. Sci Agric Sin, 1987, 20(5): 71-75. (in Chinese)
[4] 赵圣菊, 姚彩文, 霍治国. 我国赤霉病地域分布的气候分区. 中国农业科学, 1991, 24(1): 60-66.
Zhao S J, Yao C W, Huo Z G. Climatic classification of Fusarium head blight epidemic region in China. Sci Agric Sin, 1991, 24(1): 60-66. (in Chinese)
[5] Lu W Z, Chen P D.Research Progress of Wheat Scab in China. In:Proceedings of the 4th International Symposium on Fusarium head blight (C), Nanjing, China, 2012.
[6] 张宏军, 宿振起, 柏贵华, 张旭, 马鸿翔, 李腾, 邓云, 买春艳, 于立强, 刘宏伟, 杨丽, 李洪杰, 周阳. 利用Fhb1基因功能标记选择提高黄淮冬麦区小麦品种对赤霉病的抗性. 作物学报, 2018, 44: 505-511.
Zhang H J, Su Z Q, Bai G H, Zhang X, Ma H X, Li T, Deng Y, Mai C Y, Yu L Q, Liu H W, Yang L, Li H J, Zhou Y. Improvement of resistance of wheat cultivars to Fusarium head blight in the Yellow-Huai Rivers Valley Winter Wheat Zone with functional marker selection of Fhb1gene. Acta Agron Sin, 2018, 44: 505-511. (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2018.00505
[7] 程顺和, 张勇, 别同德, 高德荣, 张伯桥. 中国小麦赤霉病的危害及抗性遗传改良. 江苏农业学报, 2012, 28: 938-942.
Cheng S H, Zhang Y, Bie T D, Gao D R, Zhang B Q. Damage of wheat Fusarium head blight epidemics and genetic improvement of wheat for scab resistance in China. J Jiangsu Agric Sci, 2012, 28: 938-942. (in Chinese with English abstract)
[8] Li T, Zhang H J, Huang Y W, Su Z Q, Deng Y, Liu H W, Mai C Y, Yu G J, Li H L, Yu L Q, Zhu T Q, Yang L, Li H J, Zhou Y. Effects of the Fhb1 gene on Fusarium head blight resistance and agronomic traits of winter wheat. Crop J, 2019, 7: 799-808.
doi: 10.1016/j.cj.2019.03.005
[9] 刘宗镇, 汪志远, 赵文俊, 黄德崇, 黄晓敏, 孙锡娟. 我国改良小麦品种抗赤霉病的来源与抗赤霉病性改良中的问题. 中国农业科学, 1992, 25(4): 47-52.
Liu Z Z, Wang Z Y, Zhao W J, Huang D C, Huang X M, Sun X J. Sources of scab resistance in improved wheat varieties and problems in breeding for scab resistance in China. Sci Agric Sin, 1992, 25(4): 47-52. (in Chinese)
[10] 刘思衡, 巫升鑫, 李始明, 方毅敏, 许文真. 小麦抗赤霉病性超亲选育研究. 中国农业科学, 1998, 31(1): 40-45.
Liu S H, Wu S X, Li S M, Fang Y M, Xu W Z. Research on the breeding for transgressive inheritance of resistance to Fusarium head blight. Sci Agric Sin, 1998, 31(1): 40-45. (in Chinese)
[11] Cuthbert P A, Somers D J, Thomas J, Cloutier S, Brulé-Babel A. Fine mapping Fhb1, a major gene controlling Fusarium head blight resistance in bread wheat (Triticum aestivum L.). Theor Appl Genet, 2006, 112: 1465-1472.
doi: 10.1007/s00122-006-0249-7
[12] Cuthbert P A, Somers D J, Brulé-Babel A. Mapping of Fhb2 on chromosome 6BS: a gene controlling Fusarium head blight field resistance in bread wheat (Triticum aestivum L.). Theor Appl Genet, 2007, 114: 429-437.
doi: 10.1007/s00122-006-0439-3
[13] Xue S L, Li G Q, Jia H Y, Xu F, Lin F, Tang M Z, Wang Y, An X, Xu H B, Zhang L X, Kong Z X, Ma Z Q. Fine mapping Fhb4, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.). Theor Appl Genet, 2010, 121: 147-156.
doi: 10.1007/s00122-010-1298-5
[14] Xue S L, Xu F, Tang M Z, Zhou Y, Li G Q, An X, Lin F, Xu H B, Jia H Y, Zhang L X, Kong Z X, Ma Z Q. Precise mapping Fhb5, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.). Theor Appl Genet, 2011, 123: 1055-1063.
doi: 10.1007/s00122-011-1647-z
[15] Buerstmayr M, Steiner B, Buerstmayr H. Breeding for Fusarium head blight resistance in wheat—progress and challenges. Plant Breed, 2020, 139: 429-454.
doi: 10.1111/pbr.12797
[16] 马鸿翔, 王永刚, 高玉娇, 何漪, 姜朋, 吴磊, 张旭. 小麦抗赤霉病育种回顾与展望. 中国农业科学, 2022, 55: 837-855.
Ma H X, Wang Y G, Gao Y J, He Y, Jiang P, Wu L, Zhang X. Review and prospect on the breeding for the resistance to Fusarium. Sci Agric Sin, 2022, 55: 837-855. (in Chinese with English abstract)
[17] Zhu Z W, Hao Y F, Mergoum M, Bai G H, Humphreys G, Cloutier S, Xia X C, He Z H. Wheat breeding for resistance to Fusarium head blight in the global north: China, USA and Canada. Crop J, 2019, 7: 730-738.
doi: 10.1016/j.cj.2019.06.003
[18] 吕国锋, 别同德, 王慧, 赵仁慧, 范金平, 张伯桥, 吴素兰, 王玲, 汪尊杰, 高德荣. 长江下游麦区新育成品种(系) 3种主要病害的抗性鉴定及抗病基因/QTL的分子检测. 作物学报, 2021, 47: 2335-2347.
doi: 10.3724/SP.J.1006.2021.01097
Lyu G F, Bie T D, Wang H, Zhao R H, Fan J P, Zhang B Q, Wu S L, Wang L, Wang Z J, Gao D R. Evaluation and molecular detection of three major diseases resistance of new bread wheat varieties (lines) from the lower reaches of the Yangtze River. Acta Agron Sin, 2021, 47: 2335-2347 (in Chinese with English abstract).
[19] 朱展望, 徐登安, 程顺和, 高春保, 夏先春, 郝元峰, 何中虎. 中国小麦品种抗赤霉病基因 Fhb1的鉴定与溯源. 作物学报, 2018, 44: 473-482.
Zhu Z W, Xu D A, Cheng S H, Gao C B, Xia X C, Hao Y F, He Z H. Characterization of fusarium head blight resistance gene Fhb1 and its putative ancestor in Chinese wheat germplasm. Acta Agron Sin, 2018, 44: 473-482. (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2018.00473
[20] 姜朋, 陈小霖, 张平平, 张鹏, 姚金保, 马鸿翔. 宁麦9号对其衍生品种的遗传贡献. 作物学报, 2014, 40: 830-837.
Jiang P, Chen X L, Zhang P P, Zhang P, Yao J B, Ma H X. Genetic contribution of wheat variety Ningmai 9 to its derivatives. Acta Agron Sin, 2014, 40: 830-837 (in Chinese with English abstract).
doi: 10.3724/SP.J.1006.2014.00830
[21] 胡文静, 张春梅, 吴迪, 陆成彬, 董亚超, 程晓明, 张勇, 高德荣. 长江中下游小麦抗赤霉病品种的筛选与部分农艺性状分析. 中国农业科学, 2020, 53: 4313-4321.
Hu W J, Zhang C M, Wu D, Lu C B, Dong Y C, Cheng X M, Zhang Y, Gao D R. Screening for resistance to fusarium head blight and agronomic traits of wheat germplasms from Yangtze River region. Sci Agric Sin, 2020, 53: 4313-4321. (in Chinese with English abstract)
[22] Su Z Q, Bernardo A, Tian B, Chen H, Wang S, Ma H X, Cai S B, Liu D T, Zhang D D, Tao L, Trick H, Amand P S, Yu J M, Zhang Z Y, Bai G H. A deletion mutation in TaHRC confers Fhb1 resistance to Fusarium head blight in wheat. Nat Genet, 2019, 51: 1099-1105.
doi: 10.1038/s41588-019-0425-8
[23] Su Z Q, Jin S J, Zhang D D, Bai G H. Development and validation of diagnostic markers for Fhb1 region, a major QTL for Fusarium head blight resistance in wheat. Theor Appl Genet, 2018, 131: 2371-2380.
doi: 10.1007/s00122-018-3159-6
[24] Li G Q, Zhou J Y, Jia H Y, Gao Z X, Fan M, Luo Y J, Zhao P T, Xue S L, Li N, Yuan Y, Ma S W, Kong Z X, Jia L, An X, Jiang G, Liu W X, Cao W J, Zhang R R, Fan J C, Xu X W, Liu Y F, Kong Q Q, Zheng S H, Wang Y, Qin B, Cao S Y, Ding Y X, Shi J X, Yan H S, Wang X, Ran C F, Ma Z Q. Mutation of a histidine-rich calcium-binding protein gene in wheat confers resistance to Fusarium head blight. Nat Genet, 2019, 51: 1106-1112.
doi: 10.1038/s41588-019-0426-7
[25] 刘秉华, 杨丽, 王山荭. 矮败小麦的遗传研究. 作物学报, 1994, 20: 307-309.
Liu B H, Yang L, Wang S H. Study on genetics of dwarf-male-sterile wheat. Acta Agron Sin, 1994, 20: 306-309. (in Chinese)
[26] 刘秉华, 杨丽, 王山荭, 孟凡华. 矮败小麦群体改良的方法与技术. 作物学报, 2002, 28: 69-71.
Liu B H, Yang L, Wand S H, Meng F H. The method and technique of population improvement using dwarf-male-sterile wheat. Acta Agron Sin, 2002, 28: 69-71 (in Chinese with English abstract).
[27] 刘秉华, 杨丽. 矮败小麦及其在矮化育种中的应用. 中国农业科学, 1994, 27(5): 17-21.
Liu B H, Yang L. Dwarf-male-sterile wheat and its application in breeding for reduced plant height. Sci Agric Sin, 1994, 27(5): 17-21. (in Chinese)
[28] 刘秉华, 王山荭, 杨丽, 鲍海滢, 孟凡华, 夏兰芹, 薛国典. 不同遗传背景矮败小麦的性状表现. 作物学报, 2001, 27: 207-211.
Liu B H, Wang S H, Yang L, Bao H Y, Meng F H, Xia L Q, Xue G D. Studies on dwarf-male-sterile wheat with different genetic background. Acta Agron Sin, 2001, 27(2): 207-211. (in Chinese with English abstract)
[29] 刘秉华, 翟虎渠, 杨丽, 王山荭, 刘宏伟, 周阳, 孟凡华, 杨建平, 朱光, 崔淑兰, 张清海, 位运粮. 矮败小麦与矮败小麦育种技术. 中国农业科学, 2007, 40(S1): 73-77.
Liu B H, Zhai H Q, Yang L, Wang S H, Liu H W, Zhou Y, Meng F H, Yang J P, Zhu G, Cui S L, Zhang Q H, Wei Y L Dwarf-male-sterile wheat and its breeding technology system. Sci Agric Sin, 2007, 40(S1): 73-77 (in Chinese with English abstract).
[30] 翟虎渠, 刘秉华. 矮败小麦创制与应用. 中国农业科学, 2009, 42: 4127-4131.
Zhai H Q, Liu B H. The innovation of dwarf-male-sterile wheat and its application in wheat breading. Sci Agric Sin, 2009, 42: 4127-4131. (in Chinese with English abstract)
[31] 唐建卫, 殷贵鸿, 韩玉林, 黄峰, 王丽娜, 于海飞, 杨光宇, 李新平. 周麦16号主要农艺性状配合力及遗传效应分析. 河南农业科学, 2010, (11): 14-18.
Dang J W, Yin G H, Han Y L, Huang F, Wang L N, Yu H F, Yang G Y, Li X P. Analysis of combining ability and genetic effects of main agronomic traits of Zhoumai 16. J Henan Agric Sci, 2010, (11): 14-18. (in Chinese)
[32] 韩凤龙, 李正玲, 胡琳, 许为钢. 用于河南省小麦品种特异性和一致性鉴定的SSR分子标记研究. 中国农业科学, 2010, 43: 3968-3740.
Han F L, Li Z L, Hu L, Xu W G. Distinctness and uniformity evaluation of wheat (Triticum aestivum) varieties in Henan by SSR molecular analysis. Sci Agric Sin, 2010, 43: 3698-3704. (in Chinese with English abstract)
[33] 董冬. 小麦SSR标记辅助遗传背景选择技术研究. 中国农业科学院硕士学位论文, 北京, 2011.
Dong D. Studies on SSR Marker Assisted Selection for Genetic Background in Wheat. MS Thesis of Chinese Academy of Agricultural Sciences, Beijing, China, 2011. (in Chinese with English abstract)
[34] 代旭冉, 黄义文, 李腾, 邓云, 苏研, 买春艳, 于立强, 于广军, 李辉利, 刘宏伟, 杨丽, 周阳, 张宏军, 李洪杰. 利用Fhb1基因分子标记辅助回交育种法改良黄淮冬小麦赤霉病抗性. 麦类作物学报, 2021, 41: 1081-1089.
Dai X R, Huang Y W, Li T, Deng Y, Su Y, Mai C Y, Yu L Q, Yu G J, Li H L, Liu H W, Yang L, Zhou Y, Zhang H J, Li H J. Improvement of resistance to Fusarium head blight by Fhb1 molecular marker assisted backcrossing in the Yellow-Huai River Valley winter wheat region. J Triticeae Crops, 2021, 41: 1081-1089. (in Chinese with English abstract)
[35] 代旭冉. Fhb1、Fhb2和Fhb5基因对黄淮冬麦区小麦赤霉病抗性的影响. 河北科技师范学院硕士学位论文, 河北秦皇岛, 2011.
Dai X R. Effects of Fhb1, Fhb2, and Fhb5 on Fusarium Head Blight Resistance in the Yellow-Huai River Valley Winter Wheat Zone. MS Thesis of Hebei Normal University of Science & Technology, Qinhuangdao, Hebei, China, 2021. (in Chinese with English abstract)
[36] 李腾. Fhb1基因对黄淮冬麦区小麦品种赤霉病抗性和主要农艺性状的影响. 中国农业科学院硕士学位论文, 北京, 2019.
Li T. Effects of Fhb1 Gene on Fusarium Head Blight Resistance and Agronomic Performances in the Wheat Cultivars Adapted to the Yellow-Huai River Valley Winter Wheat Zone. MS Thesis of Chinese Academy of Agricultural Sciences, Beijing, China, 2019. (in Chinese with English abstract)
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