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作物学报 ›› 2023, Vol. 49 ›› Issue (5): 1426-1431.doi: 10.3724/SP.J.1006.2023.22025

• 研究简报 • 上一篇    下一篇

一个水稻脆秆突变体bc21的鉴定和基因定位

戴文慧1,2(), 朱琪1,2, 张小芳1,2, 吕沈阳1, 项显波3, 马涛1, 陈宇杰1, 朱世华1, 丁沃娜1,*()   

  1. 1宁波大学科学技术学院, 浙江宁波 315212
    2宁波大学海洋学院, 浙江宁波 315211
    3平阳县市场监督管理局, 浙江温州 325400
  • 收稿日期:2022-04-27 接受日期:2022-07-22 出版日期:2023-05-12 网络出版日期:2022-08-12
  • 通讯作者: *丁沃娜, E-mail: dwn@zju.edu.cn
  • 作者简介:E-mail: daiwenhui1114@163.com
  • 基金资助:
    国家自然科学基金项目(32071981);宁波市自然科学基金重点项目(202003N4016)

Identification and gene mapping of brittle culm mutant bc21 in rice

DAI Wen-Hui1,2(), ZHU Qi1,2, ZHANG Xiao-Fang1,2, LYU Shen-Yang1, XIANG Xian-Bo3, MA Tao1, CHEN Yu-Jie1, ZHU Shi-Hua1, DING Wo-Na1,*()   

  1. 1College of Science and Technology, Ningbo University, Ningbo 315212, Zhejiang, China
    2School of Marine Science, Ningbo University, Ningbo 315211, Zhejiang, China
    3Pingyang Administration for Market Regulation, Wenzhou 325400, Zhejiang, China
  • Received:2022-04-27 Accepted:2022-07-22 Published:2023-05-12 Published online:2022-08-12
  • Contact: *E-mail: dwn@zju.edu.cn
  • Supported by:
    National Natural Science Foundation of China(32071981);Key Project of Ningbo Natural Science Foundation(202003N4016)

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摘要：

利用甲基磺酸乙酯(EMS)诱变籼稻Kasalath获得一个脆秆突变体bc21。表型分析发现, 该突变体的茎秆及叶片均易折断, 且该脆性表型在苗期开始表现, 至成熟期时最显著。茎秆机械强度分析表明, bc21茎秆的抗折力、拉伸力显著下降。树脂切片及扫描电镜观察发现, bc21茎秆的厚壁组织细胞空隙增多, 细胞壁明显变薄。茎秆细胞壁组分含量分析表明, 与野生型相比, bc21的纤维素含量降低36.60%, 半纤维素和木质素含量分别升高23.08%和26.06%。经遗传分析得出, bc21的脆性性状由隐性单基因控制。利用SSR标记和自行设计的STS标记将BC21基因定位于水稻第6号染色体STS标记STS2和STS3之间约52.9 kb的范围内, 该区间内没有已报道的水稻脆性相关基因, 表明BC21可能是1个新的水稻脆秆基因, 为进一步揭示水稻茎秆机械强度的调控机制提供了材料支撑。

关键词: 水稻, 脆秆突变体, 细胞壁, 纤维素, 基因定位

Abstract:

A brittle culm mutant bc21 was obtained by mutagenesis of indica rice Kasalath with ethyl methanesulfonate (EMS). Phenotypic analysis revealed that the mutant displayed both brittle culm and leaf phenotypes, which began to manifest at seedling stage and were most pronounced at mature stage. The mechanical strength analysis showed that the breaking resistance and tensile force of bc21 stems decreased significantly. Resin sections and scanning electron microscope observation showed that the sclerenchyma cells of bc21 culms had increased voids and thinner cell walls. Compared with the wild type, the cell wall component of stems revealed that the cellulose content of bc21 decreased by 36.60%, and the hemicellulose and lignin contents increased by 23.08% and 26.06%, respectively. Genetic analysis indicated that the brittle trait of bc21 was controlled by a single recessive gene. Using SSR markers and self-designed STS markers, BC21 was located in a 52.9 kb region between the markers STS2 and STS3 on chromosome 6, and there was no previously reported rice brittleness-related gene within this region, indicating that BC21 might be a new brittle culm gene in rice. This study will provide material support for further dissection of the regulation mechanism of mechanical strength of rice stems.

Key words: rice, brittle culm mutant, cell wall, cellulose, gene mapping

图1

成熟期野生型与突变体bc21茎秆及叶片的折断表型 A: 茎秆折断表型; B: 叶片折断表型。"

图2

野生型与突变体bc21茎秆第2节间抗折力(A)与拉伸力(B)分析 *表示显著差异(P < 0.05); **表示极显著差异(P < 0.01)。"

图3

野生型与突变体bc21的茎秆横截面观察 A: 野生型茎秆横切面树脂切片观察; B: bc21茎秆横切面树脂切片观察; C: 野生型茎秆横切面扫描电镜观察; D: bc21茎秆横切面扫描电镜观察; 标尺: 100 μm (A和B)、20 μm (C和D)。"

图4

野生型与突变体bc21茎秆纤维素、半纤维素和木质素含量 *表示显著差异(P < 0.05)。"

表1

用于定位的SSR及STS标记"

标记
Marker name		 正向引物
Forward primer (5'-3')		 反向引物
Reverse primer (5'-3')
RM6734 TGAGCAGTCTGCAGATGACC GCTTGGACTTGGAGTCTTGG
STS1 ACCAGGCTGAATGTATAGAT TTAGGCACATAAACCAAG
STS2 AAAATTGTAGTGGGTTGGT TACAGAGAAAAAGATTGAAGC
STS3 CAGTGATTCGTTTGAAAT CCCTGTTGTTTGTATGAC
RM253 TCCTTCAAGAGTGCAAAACC GCATTGTCATGTCGAAGCC

图5

BC21基因在6号染色体上的精细定位"

表2

定位区间内基因及其推测功能"

基因名称Gene name 推测功能Putative function
LOC_Os06g09570 核仁GTP结合蛋白1 Nucleolar GTP-binding protein 1
LOC_Os06g09580 转座子蛋白 Transposon protein, putative
LOC_Os06g09600 表达蛋白 Expressed protein
LOC_Os06g09610 过氧化物还原酶 Peroxiredoxin
LOC_Os06g09620 表达蛋白 Expressed protein
LOC_Os06g09630 3-氧化酰基合酶 3-oxoacyl-synthase
LOC_Os06g09640 反转录转座子蛋白 Retrotransposon protein
LOC_Os06g09650 反转录转座子蛋白 Retrotransposon protein
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