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作物学报 ›› 2023, Vol. 49 ›› Issue (7): 1979-1993.doi: 10.3724/SP.J.1006.2023.24160

• 耕作栽培·生理生化 • 上一篇    下一篇

外源水杨酸提高云麻1号(Cannabis sativa L.)对铜胁迫的耐受性

项嘉铭(), 戴茜, 刘立军*()   

  1. 华中农业大学植物科学技术学院, 湖北武汉 430070
  • 收稿日期:2022-07-11 接受日期:2022-11-25 出版日期:2023-07-12 网络出版日期:2023-01-11
  • 通讯作者: *刘立军, E-mail: liulijun@mail.hzau.edu.cn
  • 作者简介:E-mail: dicerx@163.com
  • 基金资助:
    本研究由财政部和农业农村部国家现代农业产业技术体系建设专项(麻类);本研究由财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-16-E10)

Exogenous salicylic acid improves the tolerance of Yunma 1 (Cannabis sativa L.) to copper stress

XIANG Jia-Ming(), DAI Qian, LIU Li-Jun*()   

  1. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China
  • Received:2022-07-11 Accepted:2022-11-25 Published:2023-07-12 Published online:2023-01-11
  • Contact: *E-mail: liulijun@mail.hzau.edu.cn
  • Supported by:
    The China Agriculture Research System of MOF and MARA(麻类);The China Agriculture Research System of MOF and MARA(CARS-16-E10)

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摘要：

工业大麻是我国重要的天然纤维作物, 其主要种植区铜矿丰富, 耕地土壤受铜污染严重; 工业大麻有较强的耐铜性和较高的生物量, 可代替粮食作物在铜污染土壤上种植, 探究如何增强其耐铜性具有重要意义。水杨酸(SA)在植物抗逆方面有重要作用。本研究采用铜敏感品种云麻1号, 探究外源SA对铜胁迫下工业大麻耐铜性和铜富集的影响。结果表明, 铜胁迫对工业大麻具有明显毒害作用; 外源SA降低了麻纤维铜含量, 增强了根系对Cu2+的吸收和固定, 地下部铜含量是胁迫组的1610.1%, 铜累积量是胁迫组的857.1%, 这可能是通过提高半纤维素和木葡聚糖的代谢及葡萄糖苷酶的活性实现的。外源SA促进了铜胁迫下工业大麻光合作用和干物质累积, 显著增强了氧化还原酶的活性, 降低了ROS和MDA含量, 减少氧化损伤。在铜胁迫下施用外源SA, 可以特异性诱导CsCIPK25和CsWRKY32表达, 并通过调控作物离子转运、铜吸收固定、金属螯合物合成等多种途径增强铜胁迫下工业大麻的耐受性。

关键词: 工业大麻, 铜胁迫, 水杨酸, 基因表达

Abstract:

Industrial hemp is an important natural fiber crop in China. Its main planting areas are richer in copper mines, and the cultivated soil is seriously polluted by copper. Industrial hemp has strong copper tolerance and high biomass and can replace food crops on copper-contaminated soils. It is of great significance to explore how to enhance its copper tolerance. Salicylic acid (SA) plays an important role in plant stress resistance. In this study, to explore the effect of exogenous SA on the copper tolerance and copper enrichment of industrial hemp under copper stress, the copper-sensitive variety Yunma 1 was used as the experimental material. The results showed that copper stress had obvious toxic effects on industrial hemp. Exogenous SA reduced the copper content of hemp fibers and enhanced the absorption and fixation of Cu2+ in roots. The copper content in underground and the copper accumulation were 1610.1% and 857.1% in stress group, respectively, which may be achieved by improving the metabolism of hemicellulose and xyloglucan and the activity of glucosidase. Exogenous SA promoted the photosynthesis and dry matter accumulation of industrial hemp under copper stress, enhanced significantly the oxidoreductase activity, decreased ROS and MDA content, and reduced oxidative damage. The application of exogenous SA under copper stress can specifically induce the relative expression level of CsCIPK25 and CsWRKY32, and enhance the tolerance of industrial hemp under copper stress by regulating ion transport, and copper absorption immobilization, and metal chelate synthesis.

Key words: industrial hemp, copper stress, salicylic acid, the relative expression level of genes

表1

铜尾矿砂和普通土壤基本信息"

土壤
Soil		 全氮含量
Total nitrogen content
(g kg-1)		 全磷含量
Total phosphorus content (g kg-1)		 全钾含量
Total potassium content (g kg-1)		 总铜含量
Total copper content (mg kg-1)
铜尾矿砂Copper tailing sand 0.0252 0.542 0.61 1110.72
普通土壤Ordinary soil 1.9148 0.904 4.09 33.35

表2

qRT-PCR所用引物"

基因
Gene name		 正义引物
Forward primer (5°-3°)		 反义引物
Reverse primer (5°-3°)
CsCIPK25 GAAATGCTCTGCCTTGGCTAT GTGCCACCTCGTAAACCTCC
CsWRKY32 ATCATTTTGGGCATGTAACGGA TTGGCAGATGTGGATGGGTC
CsUBQ TACTGCGCCAGCTAACAAACC GCACCCGTCTGACCTGAATC

图1

铜胁迫及水杨酸喷施对苗期云麻1号根系生长的影响 C0: 无Cu处理; C1: Cu胁迫; S0: 无SA处理; S1: SA处理。不同小写字母表示处理间在0.05概率水平差异显著。"

图2

水杨酸处理对铜胁迫下云麻1号生长的影响 C0: 普通土壤; C1: Cu污染土壤; S0: 无SA处理; S1: SA处理。不同小写字母表示处理间在0.05概率水平差异显著。"

表3

外源水杨酸对铜胁迫下云麻1号生长的影响"

处理
Treatment		 株高
Plant height (cm)		 茎粗
Stem diameter (mm)		 地上部干重
Above-ground dry weight (g)		 地下部干重
Under-ground dry weight (g)
C0S0 204.71±5.76 ab 8.14±0.54 ab 27.19±0.81 a 2.53±0.50 ab
C0S1 206.11±7.24 a 8.91±0.37 a 28.51±2.68 a 2.76±0.61 a
C1S0 146.28±8.61 c 7.36±0.37 b 10.50±0.12 d 1.65±0.29 b
C1S1 195.00±6.73 b 8.90±0.58 a 22.74±1.54 bc 2.22±0.19 ab

图3

外源水杨酸对铜胁迫下苗期云麻1号活性氧清除系统的影响 处理同图1。不同小写字母表示处理间在0.05概率水平差异显著。"

图4

外源水杨酸对铜胁迫下云麻1号铜富集的影响 ACC: 地上部铜含量; UCC: 地下部铜含量; ACA: 地上部铜累积量; UCA: 地下部铜累积量; FCC: 纤维铜含量; GI: 富集系数; VI: 转运系数。处理同图2。不同小写字母表示处理间在0.05概率水平差异显著。"

图5

铜胁迫及水杨酸喷施对苗期云麻1差异基因表达的影响 A: 转录组测序各处理间差异基因表达情况; B: 外源水杨酸对铜胁迫下工业大麻差异基因表达的影响; C: 转录组测序所有处理间差异基因表达情况。处理同图1。"

图6

差异表达基因GO富集分析 A: C0S0 vs C1S0-GO; B: C1S0 vs C1S1-GO。处理同图1。"

图7

差异表达基因KEGG富集分析 A: C0S0 vs C1S0-KEGG; B: C1S0 vs C1S1-KEGG。处理同图1。"

图8

水杨酸处理对铜胁迫下云麻1号基因表达影响 处理同图1。不同小写字母表示处理间在0.05概率水平差异显著。"

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