作物学报 ›› 2023, Vol. 49 ›› Issue (8): 2051-2063.doi: 10.3724/SP.J.1006.2023.24208
李刚1,3(), 周彦辰1, 熊亚俊1, 陈伊洁1, 郭庆元1, 高杰1, 宋健2, 王俊1,*(), 李英慧3,*(), 邱丽娟3,*()
LI Gang1,3(), ZHOU Yan-Chen1, XIONG Ya-Jun1, CHEN Yi-Jie1, GUO Qing-Yuan1, GAO Jie1, SONG Jian2, WANG Jun1,*(), LI Ying-Hui3,*(), QIU Li-Juan3,*()
摘要:
大豆叶型是理想株型的重要构成部分, 对大豆品种选育具有重要意义。本研究通过对来自中国不同地区的598份材料进行Ln及其同源基因(Glyma.10G273800)单倍型分析发现, Ln基因和同源基因均分别包含3种单倍型(Hap1~Hap3)。Ln基因3种单倍型之间除了叶长没有显著差异外, Hap2在叶宽、叶面积和叶长宽比中均与Hap1/Hap3差异显著。Ln同源基因的Hap3与Hap2在不同叶型表型中均有显著差异, Hap1和Hap2之间的叶长宽比没有差异, 叶宽在第4节叶位点没有显著差异, 第5节叶位点Hap1显著低于Hap2。叶长、叶面积、叶周长Hap1均显著低于Hap2。从地理分布来看, Ln基因的Hap2主要分布在高纬度和高海拔地区, 其同源基因的3种单倍型分布未发现明显地域倾向性。遗传分析表明仅Ln基因中Hap2的D9H存在人工选择的信号, Ln基因中的另外2个突变位点和同源基因中的2个突变位点则没有人工选择的信号。本研究为大豆叶型育种提供理论依据, 同时对提高大豆产量具有积极意义。
[1] |
Heath O V S, Gregory F G. The constancy of the mean net assimilation rate and its ecological importance. Ann Bot (London) 1938, 2: 811-818.
doi: 10.1093/oxfordjournals.aob.a084036 |
[2] |
Board J E, Harville B G. Explanations for greater light interception in narrow-vs. wide-row. Crop Sci, 1992, 32: 198-202.
doi: 10.2135/cropsci1992.0011183X003200010041x |
[3] |
Srinivasan V, Kumar P, Long S P. Decreasing, not increasing, leaf area will raise crop yields under global atmospheric change. Global Change Biol, 2017, 23: 1626-1635.
doi: 10.1111/gcb.13526 pmid: 27860122 |
[4] | 陈磊.大豆叶形调控基因的图位克隆与功能分析. 南京农业大学博士学位论文, 江苏南京, 2014. |
Chen L.Atlas Cloning and Functional Analysis of Soybean Leaf Shape Regulatory Genes. PhD Dissertation of Nanjing Agricultural University, Nanjing, Jiangsu, China, 2014. (in Chinese with English abstract) | |
[5] |
Baldocchi D D, Verma S B, Rosenberg N J, Blad B L, Specht J E. Microclimate-plant architectural interactions: influence of leaf width on the mass and energy exchange of a soybean canopy. Agric Forest Meteorol, 1985, 35: 1-20.
doi: 10.1016/0168-1923(85)90070-X |
[6] |
Takahashi N. Linkage relation between the genes for the forms of leaves and the number of seeds per pod of soybeans. Jpn J Genet, 1934, 9: 208-225.
doi: 10.1266/jjg.9.208 |
[7] | Johnson H W, Bernard R L. Soybean genetics and breeding. Adv Agron, 1962, 14: 149-221. |
[8] |
Jeong N, Moon J K, Kim H S, Kim C G, Jeong S C. Fine genetic mapping of the genomic region controlling leaflet shape and number of seeds per pod in the soybean. Theor Appl Genet, 2011, 122: 865-874.
doi: 10.1007/s00122-010-1492-5 pmid: 21104397 |
[9] | Sayama T, Tanabata T, Saruta M, Yamada T, Anai T, Kaga A, Ishimoto M. Confirmation of the pleiotropic control of leaflet shape and number of seeds per pod by the Ln gene in induced soybean mutants. Breed Sci, 2017: 16201. |
[10] |
Mandl F A, Buss G R. Comparison of narrow and broad leaflet isolines of soybean. Crop Sci, 1981, 21: 25-27.
doi: 10.2135/cropsci1981.0011183X002100010007x |
[11] |
Robinson J M. Leaflet photosynthesis rate and carbon metabolite accumulation patterns in nitrogen-limited, vegetative soybean plants. Photosynth Res, 1996, 50: 133-148.
doi: 10.1007/BF00014884 |
[12] |
Sung F J M, Chen J J. Changes in photosynthesis and other chloroplast traits in lanceolate leaflet isoline of soybean. Plant Physiol, 1989, 90: 773-777.
doi: 10.1104/pp.90.2.773 pmid: 16666842 |
[13] | 田佩占. 大豆育种的叶形问题. 遗传学报, 1977, 4: 22-30. |
Tian P Z. Leaf shape problems in soybean breeding. Acta Genet Sin, 1977, 4: 22-30. (in Chinese with English abstract) | |
[14] | 游明安, 马国荣, 刘佑斌, 盖钧镒, 邱家驯. 大豆叶形、短叶柄近等基因系的选育与利用. 中国油料, 1995, 17(4): 7-9. |
You A M, Ma G R, Liu Y B, Gai J Y, Qiu J X. Selection and utilization of soybean leaf-shaped, short petiole proximal and other gene lines. Chin J Oil Crop Sci, 1995, 17(4): 7-9. (in Chinese with English abstract) | |
[15] | 周勋波, 吴海燕. 关于大豆理想株型的探讨. 大豆通报, 2002, (5): 4-5. |
Zhou X B, Wu H Y. Discussion on the ideal plant type of soybean. Soybean Sci Technol, 2002, (5): 4-5. (in Chinese with English abstract) | |
[16] |
Yamanaka N, Ninomiya S, Hoshi M, Tsubokura Y, Yano M, Nagamura Y, Sasaki T, Harada K. An informative linkage map of soybean reveals QTLs for flowering time, leaflet morphology and regions of segregation distortion. DNA Res, 2001, 8: 61-72.
doi: 10.1093/dnares/8.2.61 pmid: 11347903 |
[17] |
Kim H K, Kang S T, Suh D Y. Analysis of quantitative trait loci associated with leaflet types in two recombinant inbred lines of soybean. Plant Breed, 2005, 124: 582-589.
doi: 10.1111/pbr.2005.124.issue-6 |
[18] |
Jun T H, Freewalt K, Michel A P, Mian R. Identification of novel QTL for leaf traits in soybean. Plant Breed, 2014, 133: 61-66.
doi: 10.1111/pbr.2014.133.issue-1 |
[19] |
Vieira A J D, Oliveira D A, Soares T C B, Schuster I, Piovesan N D, Martinez C A, Barros E G, Moreira M A. Use of the QTL approach to the study of soybean trait relationships in two populations of recombinant inbred lines at the F7 and F8 generations. Braz J Plant Physiol, 2006, 18: 281-290.
doi: 10.1590/S1677-04202006000200004 |
[20] |
Mian M A R, Ashley D A, Boerma H R. An additional QTL for water use efficiency in soybean. Crop Sci, 1998, 38: 390-393.
doi: 10.2135/cropsci1998.0011183X003800020020x |
[21] |
Orf J H, Chase K, Jarvik T, Mansur L M, Cregan P B, Adler F R, Lark K G.Genetics of soybean agronomic traits: I. Comparison of three related recombinant inbred populations. Crop Sci, 1999, 39: 1642-1651.
doi: 10.2135/cropsci1999.3961642x |
[22] |
Mansur L M, Lark K G, Kross H, Oliveira A. Interval mapping of quantitative trait loci for reproductive, morphological, and seed traits of soybean (Glycine max L.). Theor Appl Genet, 1993, 86: 907-913.
doi: 10.1007/BF00211040 pmid: 24193996 |
[23] |
Mansur L M, Orf J H, Chase K, Jarvik T, Cregan P B, Lark K G. Genetic mapping of agronomic traits using recombinant inbred lines of soybean. Crop Sci, 1996, 36: 1327-1336.
doi: 10.2135/cropsci1996.0011183X003600050042x |
[24] |
Keim P, Diers B W, Olson T C, Shoemaker R C. RFLP mapping in soybean: association between marker loci and variation in quantitative traits. Genetics, 1990, 126: 735-742.
doi: 10.1093/genetics/126.3.735 pmid: 1979039 |
[25] |
Chen Q S, Zhang Z C, Liu C Y, Xin D W, Qiu H M, Shan C P, Shan C Y, Hu G H. QTL analysis of major agronomic traits in soybean. Agric Sci China, 2007, 6: 399-405.
doi: 10.1016/S1671-2927(07)60062-5 |
[26] | Bernard R L, Weiss M G. Qualitative genetics. In: Boerma H R, Specht J E, eds. Soybeans: Improvement, Production, and Uses. Madison: American Society of Agronomy, 1973. pp 117-154. |
[27] |
Jeong N, Suh S J, Kim M H, Lee S, Moon J K, Kim H S, Jeong S C. Ln is a key regulator of leaflet shape and number of seeds per pod in soybean. Plant Cell, 2012, 24: 4807-4818.
doi: 10.1105/tpc.112.104968 |
[28] |
Fang C, Li W Y, Li G Q, Wang Z, Zhou Z K, Ma Y M, Shen Y T, Li C C, Wu Y W, Zhu B G, Yang W C, Tian Z X. Cloning of Ln gene through combined approach of map-based cloning and association study in soybean. J Genet Genomics, 2013, 40: 93-96.
doi: 10.1016/j.jgg.2013.01.002 |
[29] |
Li Y H, Qin C, Wang L, Jiao C Z, Hong H L, Tian Y, Li Y F, Xing G N, Wang J, Gu Y Z, Gao X P, Li D L, Li H Y, Liu Z X, Jing X, Feng B B, Zhao T, Guan R X, Guo Y, Liu J, Yan Z, Zhang L J, Ge T L, Li X K, Wang X B, Qiu H M, Zhang W H, Luan X Y, Han Y P, Han D Z, Chang R Z, Guo Y L, Reif J C, Jackson S A, Liu B, Tian S L, Qiu L J. Genome-wide signatures of the geographic expansion and breeding of soybean. Sci China Life Sci, 2022, 66: 350-365.
doi: 10.1007/s11427-022-2158-7 |
[30] |
Schmutz J, Cannon S B, Schlueter J, Ma J X, Mitros T, Nelson W, Hyten, D L, Song Q J, Telen J J, Cheng J L, Xu D, Hellsten U, May G D, Yu Y, Sakurai T, Umezawa T, Bhattacharyya M K, Sandhu D, Valliyodan B, Lindquist E, Peto M, Grant D, Shu S Q, Goodstein D. Genome sequence of the palaeopolyploid soybean. Nature, 2010, 463: 178-183.
doi: 10.1038/nature08670 |
[31] |
张洪映, 毛新国, 景蕊莲, 谢惠民, 昌小平. 小麦 TaPK7 基因单核苷酸多态性与抗旱性的关系. 作物学报, 2008, 34: 1537-1543.
doi: 10.3724/SP.J.1006.2008.01537 |
Zhang H Y, Mao X G, Jing R L, Xie H M, Chang X P. Relationship between single nucleotide polymorphisms and drought resistance in TaPK7 gene in wheat. Acta Agron Sin, 2008, 34: 1537-1543. (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2008.01537 |
|
[32] |
Stephens J C, Schneider J A, Tanguay D A, Choi J, Acharya T, Stanley S E, Jiang R, Messer C J, Chew A, Han J H, Duan J, Carr J L, Lee M S, Koshy B, Kumar A M, Zhang G, Newell W R, Windemuth A, Xu C B, Kalbflesch T S, Shaner S L, Arnold K, Schulz V, Drysdale C M, Nandabalan K, Judson R S, Ruano G, Vovis G F. Haplotype variation and linkage disequilibrium in 313 human genes. Science, 2001, 293: 489-493.
doi: 10.1126/science.1059431 pmid: 11452081 |
[33] |
张乐, 李英慧, 刘章雄, 邱丽娟. 栽培大豆(G. max)和野生大豆(G. soja)的Glyma13g21630基因多样性. 作物学报, 2011, 37: 1724-1734.
doi: 10.3724/SP.J.1006.2011.01724 |
Zhang L, Li Y H, Liu Z X, Qiu L J. Glyma13g21630 genetic diversity of cultivated soybeans (G. max) and wild soybeans (G. soja). Acta Agron Sin, 2001 37: 1724-1734. (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2011.01724 |
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