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Acta Agron Sin ›› 2008, Vol. 34 ›› Issue (10): 1843-1848.doi: 10.3724/SP.J.1006.2008.01843

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Cloning a New Member from Glu-B3 Locus in Durum Wheat and Deve- loping Its Specific Marker

ZHAO Yong-Tao12,KONG Xiu-Ying2*,ZHAN Ke-Hui1*   

  1. 1 College of Agronomy, Henan Agricultural University, Zhengzhou 450002, Henan; 2 Key Laboratory of Crop Germplasm and Biotechnology, Minis-try of Agriculture / National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Sciences, Chinese Academy of Agri-cultural Sciences, Beijing 100081, China
  • Received:2008-03-11 Revised:1900-01-01 Online:2008-10-12 Published:2008-10-12
  • Contact: ZHAN Ke-Hui

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Abstract: A new member of Glu-B3 gene family was isolated from durum wheat Langdon variety. According to the alignment of the conserved N- and C- terminal domains of the deduced amino-acid of LMW-GS genes in GenBank, a pair of degenerate primers was designed. Using this primer pair, an M-type LMW-GS gene was identified from Langdon. The gene, designated as LMWLDN-M, encodes 350 amino acids. Compared with other sequences of Glu-3 isolated from Langdon in our laboratory, a SNP was detected and thus a gene specific marker was developed. This marker can also be used to map the gene LMWLDN-M onto the chromosome 1B using LDN D-genome disomic substitution lines and CS nulli-tetrasomic lines as templates. By comparison with other members of Langdon isolated before, we confirm that LMWLDN-M is a new member of Glu-B3 family. After sequencing the BAC carrying this gene, it can help us to understand the complex Glu-3 loci well.

Key words: Durum wheat, Low-molecular-weight glutenin subunit, Gene family, Homology-based cloning

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