Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2008, Vol. 34 ›› Issue (12): 2092-2098.doi: 10.3724/SP.J.1006.2008.02092

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Expression Characteristics of Tobacco Tapetum-Specific Promoter pTA29 in Cotton

YIN Meng-Hui,DONG Jing,LI Xian-Bi,HUO Lei,LUO Ming,LI De-Mou,PEI Yan,XIAO Yue-Hua*   

  1. Biotechnology Research Center, Southwest University / Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Chongqing, 400716, China
  • Received:2008-03-26 Revised:2008-07-13 Online:2008-12-12 Published:2008-10-10
  • Contact: XIAO Yue-Hua

PDF

2052

Cited

1

Abstract:

Tobacco tapetum-specific promoter pTA29 is widely used to create male sterile lines and to study pollen development in various plants. However, the pTA29 expression characteristics in cotton is still not elucidated. To determine the expression characteristics of pTA29 promoter in cotton, gus gene was fused downstream to pTA29, transformed into cotton. It was demonstrated that 10% alcohol suppressed the intrinsic GUS-like activity in cotton anther. GUS staining with modified solution showed that the GUS activity in the pTA29:gus transformants mainly exited in anthers with two peaks in the floral buds of 6 and 15 mm, while no GUS activity was detected in roots, stems, ovules, petals and bracts. Inconsistent with that in tobacco, the GUS activity was also detected in the leaf trichomes and pollens in pTA29:gus transformants. Real-time RT-PCR analysis indicated that the gus transcripts accumulated in a same pattern with the GUS activity. These results suggest that the tobacco tapetum-specific promoter pTA29 can drive the downstream genes to express preferentially in anthers of cotton. The application of pTA29 in cotton male sterility engineering was discussed.

Key words: pTA29, Cotton, Intrinsic GUS-like activity, Expression Specificity

[1]Mariani C, De Beuckeleer M, Truettner J, Leemans J, Gold-berg R B. Induction of male sterility in plants by a chimaeric ribonuclease gene. Nature, 1990, 347: 737-741
[2]Xu F(徐芳), Xiong A-S(熊爱生), Peng R-H(彭日荷), Hou X-L(侯喜林), Yao Q-H(姚泉洪). Progress in the study on conditional male sterility in plants by genetic engineering. Hereditas(Beijing)(遗传), 2006, 28(4): 507-510(in Chinese with English abstract)
[3]Yang Z-L(杨泽良), Dang X-M(党选民), Cao Z-M(曹振木), Hu K-L(胡开林). The application of gene engineering on creating male sterility in plants. Chin Agric Sci Bull (中国农学通报), 2005, 21(10): 25-29(in Chinese with English abstract)
[4]Koltunow A M, Truettner J, Cox K H, Wallroth M, Goldberg R B. Different temporal and spatial gene expression patterns occur during anther development. Plant Cell, 1990, 2: 1201-1224
[5]Denis M, Delourme R, Gourret J P, Mariani C, Renard M. Expression of engineered nuclear male sterility in Brassica napus. Plant Physiol, 1993, 101: 1295-1304
[6]Huang S, Cerny R E, Qi Y, Bhat D, Aydt C M, Hanson D D, Malloy K P, Ness L A. Transgenic studies on the involvement of cytokinin and gibberellin in male development. Plant Physiol, 2003, 131: 1270-1282
[7]Zhang Y-M(张玉满), Wang H-Y(王寰宇), Liu Y-L(刘玉乐). Gene Engineering Insect Resistant Hybrid Cotton (基因工程抗虫杂交棉). In: Jia S-R(贾士荣), Guo S-D(郭三堆), An D-C(安道昌), eds. Transgenic Cotton (转基因棉花), Beijing: Science Press, 2001. pp 235-240(in Chinese)
[8]Zhang H-J(张惠军), Wang H-Y(王寰宇), Shi Y-J(石跃进), Zhang Y-M(张玉满), Yue J-X(岳见雄), Wu S-J(吴慎杰), Zhu Y-H(朱永红), Liu Y-L(刘玉乐), Yang H-Y(杨怀义). Cotton genetic tranformation of barnase male sterility gene. Cotton Sci (棉花学报), 2007, 19(4): 261-266 (in Chinese with Eng-lish abstract)
[9]Song H-Y(宋洪元), Cao B-H(曹必好), Ding J-G(丁建刚), Lei J-J(雷建军), Song M(宋明). Constructions of male steril-ity gene and fertility restoring gene expression vectors by Cre/loxp site-specific recombination system. J Agric Bio-technol (农业生物技术学报), 2004, 12(4): 396-400 (in Chi-nese with English abstract)
[10]Luo M, Xiao Y H, Li X B, Lu X F, Deng W, Li D M, Hou L, Hu M Y, Li Y, Pei Y. GhDET2, a steroid 5α-reductase, plays an important role in cotton fiber cell initiation and elongation. Plant J, 2007, 51: 419-430
[11]Xiao Y H, Li X B, Yang X Y, Luo M, Hou L, Guo S H, Luo X Y, Pei Y. Cloning and characterization of a balsam pear class I chitinase gene (Mcchit1) and its etopic expression enhaces fungal resistance in transgenic plants. Biosci Biotechnol Bio-chem, 2007, 71: 1211-1219
[12]Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant, 1962, 15: 473-497
[13]Shenk R U, Hildebrandt A C. Medium and techniques for in-duction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot, 1972, 50: 199-204
[14]Li Y-E(李燕娥), Zhu Z(朱祯), Wu X(吴霞), Meng J-H(孟晋红), Fan X-P(范小平), Wu J-H(吴家和), He J-X(何鉴星), Shi G-C(史高川), Xiao J-L(肖娟丽), Zhang H-X(张换样). Report on grafts of transgenic cotton. China Cotton (中国棉花), 2000, 27(3): 25-30(in Chinese)
[15]Xiao Y-H(肖月华), Luo M(罗明), Fang W-G(方卫国), Luo K-M(罗克明), Hou L(侯磊), Li D-M(李德谋), Pei Y(裴炎). PCR walking in cotton genome using YADE method. Acta Genet Sin (遗传学报), 2002, 29(1): 62-66 (in Chinese with English abstract)
[16]Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory Press, 1989
[17]Zhu Y Q, Xu K X, Luo B, Wang J W, Chen X Y. An ATP-binding cassette transporter GhWBC1 from elongating cotton fibers. Plant Physiol, 2003, 133: 580-588
[18]Jefferson R A, Kavanagh T A, Bevan M W. GUS fusions: β-glucuronidaseas a sensitive gene fusion marker in higher plants. EMBO J, 1987, 6: 3901-3907
[19]Hu Q Y, Chee P P, Miller P D. Intrinsic GUS-like activity in soybean. Soybean Sci, 1991, 10: 200-204
[20]Plegt L, Bino R J. β-Glucuronidase activity during develop-ment of the male gametophyte from transgenic and non-transgenic plants. Mol Gen Genet, 1989, 216: 321-327
[21]Kosugi S, Ohashi Y, Nakajima K, Arai Y. An improved assay for β-glucuronidase in transformed cells: Methanol almost completely suppresses a putative endogenous β-glucuronidase activity. Plant Sci, 1990, 70: 133-140
[22]Burgess D G, Ralston E J, Hanson W G, Heckert M, Ho M, Jenq T, Palys J M, Tang K, Gutterson N. A novel, two-component system for cell lethality and its use in engi-neering nuclear male-sterility in plants. Plant J, 2002, 31: 113-125
[23]Kapoor S, Kobayashi A, Takatsuji H. Silencing of the tapetum-specific zinc finger gene TAZ1 causes premature de-generation of tapetum and pollen abortion in Petunia. Plant Cell, 2002, 14: 2353-2367
[24]Mansoor S, Amin I, Hussain M, Zafar Y, Briddon R W. En-gineering novel traits in plants through RNA interference. Trends Plant Sci, 2006, 11: 559-565
[25]Wendel J F, Cronn R C, Johnston J S, Price H J. Feast and famine in plant genomes. Genetica, 2002, 115: 37-47
[1] ZHOU Jing-Yuan, KONG Xiang-Qiang, ZHANG Yan-Jun, LI Xue-Yuan, ZHANG Dong-Mei, DONG He-Zhong. Mechanism and technology of stand establishment improvements through regulating the apical hook formation and hypocotyl growth during seed germination and emergence in cotton [J]. Acta Agronomica Sinica, 2022, 48(5): 1051-1058.
[2] SUN Si-Min, HAN Bei, CHEN Lin, SUN Wei-Nan, ZHANG Xian-Long, YANG Xi-Yan. Root system architecture analysis and genome-wide association study of root system architecture related traits in cotton [J]. Acta Agronomica Sinica, 2022, 48(5): 1081-1090.
[3] YAN Xiao-Yu, GUO Wen-Jun, QIN Du-Lin, WANG Shuang-Lei, NIE Jun-Jun, ZHAO Na, QI Jie, SONG Xian-Liang, MAO Li-Li, SUN Xue-Zhen. Effects of cotton stubble return and subsoiling on dry matter accumulation, nutrient uptake, and yield of cotton in coastal saline-alkali soil [J]. Acta Agronomica Sinica, 2022, 48(5): 1235-1247.
[4] ZHENG Shu-Feng, LIU Xiao-Ling, WANG Wei, XU Dao-Qing, KAN Hua-Chun, CHEN Min, LI Shu-Ying. On the green and light-simplified and mechanized cultivation of cotton in a cotton-based double cropping system [J]. Acta Agronomica Sinica, 2022, 48(3): 541-552.
[5] ZHANG Yan-Bo, WANG Yuan, FENG Gan-Yu, DUAN Hui-Rong, LIU Hai-Ying. QTLs analysis of oil and three main fatty acid contents in cottonseeds [J]. Acta Agronomica Sinica, 2022, 48(2): 380-395.
[6] ZHANG Te, WANG Mi-Feng, ZHAO Qiang. Effects of DPC and nitrogen fertilizer through drip irrigation on growth and yield in cotton [J]. Acta Agronomica Sinica, 2022, 48(2): 396-409.
[7] ER Chen, LIN Tao, XIA Wen, ZHANG Hao, XU Gao-Yu, TANG Qiu-Xiang. Coupling effects of irrigation and nitrogen levels on yield, water distribution and nitrate nitrogen residue of machine-harvested cotton [J]. Acta Agronomica Sinica, 2022, 48(2): 497-510.
[8] ZHAO Wen-Qing, XU Wen-Zheng, YANG Liu-Yan, LIU Yu, ZHOU Zhi-Guo, WANG You-Hua. Different response of cotton leaves to heat stress is closely related to the night starch degradation [J]. Acta Agronomica Sinica, 2021, 47(9): 1680-1689.
[9] YUE Dan-Dan, HAN Bei, Abid Ullah, ZHANG Xian-Long, YANG Xi-Yan. Fungi diversity analysis of rhizosphere under drought conditions in cotton [J]. Acta Agronomica Sinica, 2021, 47(9): 1806-1815.
[10] ZENG Zi-Jun, ZENG Yu, YAN Lei, CHENG Jin, JIANG Cun-Cang. Effects of boron deficiency/toxicity on the growth and proline metabolism of cotton seedlings [J]. Acta Agronomica Sinica, 2021, 47(8): 1616-1623.
[11] GAO Lu, XU Wen-Liang. GhP4H2 encoding a prolyl-4-hydroxylase is involved in regulating cotton fiber development [J]. Acta Agronomica Sinica, 2021, 47(7): 1239-1247.
[12] MA Huan-Huan, FANG Qi-Di, DING Yuan-Hao, CHI Hua-Bin, ZHANG Xian-Long, MIN Ling. GhMADS7 positively regulates petal development in cotton [J]. Acta Agronomica Sinica, 2021, 47(5): 814-826.
[13] XU Nai-Yin, ZHAO Su-Qin, ZHANG Fang, FU Xiao-Qiong, YANG Xiao-Ni, QIAO Yin-Tao, SUN Shi-Xian. Retrospective evaluation of cotton varieties nationally registered for the Northwest Inland cotton growing regions based on GYT biplot analysis [J]. Acta Agronomica Sinica, 2021, 47(4): 660-671.
[14] ZHOU Guan-Tong, LEI Jian-Feng, DAI Pei-Hong, LIU Chao, LI Yue, LIU Xiao-Dong. Efficient screening system of effective sgRNA for cotton CRISPR/Cas9 gene editing [J]. Acta Agronomica Sinica, 2021, 47(3): 427-437.
[15] HAN Bei, WANG Xu-Wen, LI Bao-Qi, YU Yu, TIAN Qin, YANG Xi-Yan. Association analysis of drought tolerance traits of upland cotton accessions (Gossypium hirsutum L.) [J]. Acta Agronomica Sinica, 2021, 47(3): 438-450.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No.12 Zhongguancun South Street, Beijing 100081,China Email:xbzw@chinajournal.net.cn
Supported by Beijing Magtech Co.Ltd