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Acta Agron Sin ›› 2008, Vol. 34 ›› Issue (12): 2092-2098.doi: 10.3724/SP.J.1006.2008.02092

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Expression Characteristics of Tobacco Tapetum-Specific Promoter pTA29 in Cotton

YIN Meng-Hui,DONG Jing,LI Xian-Bi,HUO Lei,LUO Ming,LI De-Mou,PEI Yan,XIAO Yue-Hua*   

  1. Biotechnology Research Center, Southwest University / Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Chongqing, 400716, China
  • Received:2008-03-26 Revised:2008-07-13 Online:2008-12-12 Published:2008-10-10
  • Contact: XIAO Yue-Hua

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Abstract:

Tobacco tapetum-specific promoter pTA29 is widely used to create male sterile lines and to study pollen development in various plants. However, the pTA29 expression characteristics in cotton is still not elucidated. To determine the expression characteristics of pTA29 promoter in cotton, gus gene was fused downstream to pTA29, transformed into cotton. It was demonstrated that 10% alcohol suppressed the intrinsic GUS-like activity in cotton anther. GUS staining with modified solution showed that the GUS activity in the pTA29:gus transformants mainly exited in anthers with two peaks in the floral buds of 6 and 15 mm, while no GUS activity was detected in roots, stems, ovules, petals and bracts. Inconsistent with that in tobacco, the GUS activity was also detected in the leaf trichomes and pollens in pTA29:gus transformants. Real-time RT-PCR analysis indicated that the gus transcripts accumulated in a same pattern with the GUS activity. These results suggest that the tobacco tapetum-specific promoter pTA29 can drive the downstream genes to express preferentially in anthers of cotton. The application of pTA29 in cotton male sterility engineering was discussed.

Key words: pTA29, Cotton, Intrinsic GUS-like activity, Expression Specificity

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