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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (4): 749-754.doi: 10.3724/SP.J.1006.2009.00749

• RESEARCH NOTES • Previous Articles     Next Articles

Comparison on Gene Expression and Microstructure of Tobacco Leaves at Maturity Stage in Different Types of Soil in southern Anhui Province,China

QIU Li-You1,LI Fu-Xin2,ZU Chao-Long3,MA Chen-Xin3,YANG Chao2,FENG Yun1,LIU Guo-Shun2,ZHAO Hui-Jie1*   

  1. 1College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,China;2Agronomy College,Henan Agricultural University,Zhengzhou 450002,China;3Anhui Wannan Tobacco Leaf Co.Ltd.,Xuancheng 242000,China
  • Received:2008-06-04 Revised:2008-12-15 Online:2009-04-12 Published:2009-02-16
  • Contact: ZHAO Hui-Jie

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Abstract:

Yunyan 97(Nicotiana tabacum) was planted in four types of soil including coarse sandy soil, coarse sandy mud, powder sandy soil, and rice soil (CK), in the south of Anhui. The gene expression profile and cell construction of tobacco leaves at maturity stage were explored using cDNA microarray and microscopical technique. Comparing tobacco leaves grown in coarse sandy soil to rice soil, up-regulated genes were mainly for cell multiplication, growth, development, and oxidation stress, while genes in photosynthesis, protein and phospholipids synthesis were down-regulated. Specifically up-regulated genes involved in auxin transport, polysaccharide synthesis, and responsible for protein and amino acid degradation in tobacco leaves cultivated in coarse sandy mud briefly compared with rice soil. Compared to rice soil, up-regulated genes of tobacco leaves in powder sandy soil principally dealt with drought stressand degrading cellulose, pectin, starch, and protein. The microscopic and ultramicroscopic structure of the tobacco leaves revealed that tobacco leaves in powder sandy soil and rice soil grew badly. On the contrary, coarse sandy mud and especially coarse sandysoil produced well grown leaves. The results should conduce to unveil the mechanism of burnt-sweet sensation formation in tobacco leaves cultivated in the south of Anhui Province.

Key words: Tobacco, Soil type, Maturity stage, cDNA microarray, Microstructure

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