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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (6): 1013-1020.doi: 10.3724/SP.J.1006.2009.01013

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation,Characterization and Mapping of Genomic Microsatellite Markers for the First Time in Sea-Island Cotton(Gossypium barbadense)

ZHANG Pei-Pei,WANG Xia-Qing**,YU Yang,YU Yu,LIN Zhong-Xu*,ZHANG Xian-Long   

  1. Department of Plant Science & Technology,National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research(Wuhan),Huazhong Agricultural University,Wuhan 430070,China
  • Received:2008-09-16 Revised:2009-02-15 Online:2009-06-12 Published:2009-04-16
  • Contact: LIU Zhong-Xu，E-mail:linzhongxu@mail.hzau.edu.cn;Tel:027-87283955;Fax:027-87280016

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Abstract:

Sea-island cotton (G. barbadense) is one of the most important cultivated cotton species in the world. In order to explore the genetic diversity of this species, microsatellite loci were identified from G. barbadense cv. Pima3-79 (the genetic standard line). Microsatellite markers were developed using two different approaches: (i) cloning of ISSR amplified fragments and (ii) amplification using degenerate primers. Two hundred and thirty-nine unique clones were generated from 1447 recombinants, and 214 unique sequences were obtained. Eighty-six primer pairs were developed from 70 sequences that had flanking regions sufficient for primer design. The 86 SSR primer pairs were used to analyze 56 sea-island cotton accessions and 4 upland cotton cultivars, 16 primers had no amplification and 43 primers did not detect polymorphism between all the cultivars. Nineteen primers showed polymorphism between the sea-island cotton accessions. On the basis of Jaccard’s genetic similarity coefficient, these primers could distinctly distinguish sea-island cotton and upland cotton, and sea-island cotton accessions were separated into four groups. Nine interspecific polymorphic markers were mapped on the cotton genetic map with four mapped at A sub-genome and five at D sub-genome. These microsatellite markers will be useful for assessing the genetic diversity patterns within sea-island cotton as well as aiding in construction of genetic linkage maps.

Key words: Sea-island cotton, Microsatellites, Genetic variation, Genetic mapping


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