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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (12): 2213-2217.doi: 10.3724/SP.J.1006.2009.02213

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Detection of QTL for Resistance to Rice Black-Streaked Dwarf  Disease

PAN Cun-Hong1,2,LI Ai-Hong2,**,CHEN Zong-Xiang1,WU Lin-Bo1,DAI Zheng-Yuan2, HUANG Nian-Sheng2,CHEN Xi-Jun1,ZHANG Ya-Fang1,ZUO Shi-Min1,PAN Xue-Biao1*   

  1. 1Key Laboratory of Plant Functional Genomics of Ministry of Education/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province,Yangzhou University,Yangzhou 225009,China；2Lixiahe Agricultural Research Institute of Jiangsu Province/Nanjing Subcenter of National Rice Improvement Center，Yangzhou 225007，China
  • Received:2009-03-11 Revised:2009-06-25 Online:2009-12-10 Published:2009-09-10
  • Contact: PAN Xue-Biao, E-mail: shuidao@yzu.edu.cn; Tel: 0514-87972136

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Abstract:

The rice black-streaked dwarf viral disease (RBSDV), transmitted by the planthopper Laodelphax striatellus Fallèn, was one of the most serious diseases in rice in south China. Breeding of RBSDV resistance variety using marker-assisted selection was effective measure to reduce the disease damage. For achieving this aim, QTL mapping of resistance to RBSDV was studied using a recombinant inbred line (RIL) population from the cross between Zhenshan 97B and Minghui 63. Reactions of 242 RILs to RBSDV were investigated by natural infection method in Agricultural College of Yangzhou University and Lixiahe Agricultural Research Institute, and scored by the incidence of RBSDV. The trait of RBSDV incidence was normally distributed and marked bias towards the resistant parent, which implied that they were controlled by quantitative trait loci. Six QTLs for resistance to RBSDV were detected by WinQTLcart 2.5 software. Two adjacent QTLs were mapped on chromosome 6, two on chromosome 11, one on chromosomes 7 and 9, respectively. Four QTLs on chromosomes 6, 7, and 9 could be detected in two locations, and were stably expressed across the two environments. There had not been any reports about location of QTL for resistance of rice stripe virus and planthopper, which suggested that six QTLs in this study were really resistant to RBSDV. Two QTLs on chromosome 6 were with main effect, and their contributions to the total variation were 20.20% and 18.68% with LOD scores of 12.09 and 9.77 respectively, moreover, they had high value for study on genetics of RBSDV and should be useful in marker-assisted selection for resistance to RBSDV.

Key words: Rice black-streaked dwarf viral disease, Recombinant inbred line population, QTL Analysis

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