Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (12): 2128-2135.doi: 10.3724/SP.J.1006.2014.02128

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of QTLs for Plant Type Traits in Rice (Oryza sativa)

ZHANG Ling**,LI Xiao-Nan**,WANG Wei,YANG Sheng-Long,LI Qing,WANG Jia-Yu*   

  1. Rice Research Institute, Shenyang Agricultural University / Key Laboratory of Northeast Rice Biology and Breeding, Ministry of Agriculture, Shenyang 110866, China
  • Received:2014-04-16 Revised:2014-09-16 Online:2014-12-12 Published:2014-10-16
  • Contact: 王嘉宇, E-mail: ricewjy@126.com; Tel: 024-88487183

Abstract:

A recombinant inbred lines (RILs) derived from the across between indica restorer line Luhui 99 and super japonica cultivar Shennong 265, was used to analyze the quantitative trait loci (QTLs) for plant height, panicle length, tillers, and leaf traits in 2012 and 2013. A total of 39 QTLs were detected on chromosomes 1, 2, 3, 6, 7, 8, and 9 with LOD score ranging from 2.50 to 16.90. Eleven of them were detected in both 2012 and 2013. Moreover, QTL clusters were detected on chromosomes 1, 6 and 9, which may be related to significant or highly significant correlations between plant type traits. Among them, the QTL cluster on chromosome 9 contained four QTLs, qPH9, qPL9, qFLL9, and qSLL9 in the interval between RM3700 and RM7424, and the four QTLs were detected in both years. In addition, five major QTLs were first reported, among which three QTLs (qPH8, qFLW6, and qSLW6) had the larger effect. The results facilitate further understanding of the genetic basis for plant height, panicle length, tillers and leaf traits.

Key words: Oryza sativa L., Plant type traits, Different environments, QTL analysis

[1]陈温福, 徐正进. 水稻超高产育种理论与方法. 北京: 科学出版社, 2007. pp 1–16



Chen W F, Xu Z J. The Theories and Methods of Rice for Maximum Yield. Beijing: Science Press, 2007. pp 1–16 (in Chinese)



[2]徐正进, 林晗, 马殿荣, 王嘉宇, 徐海, 赵明辉, 陈温福. 北方粳稻穗型改良理论与技术研究及应用. 沈阳农业大学学报, 2012, 43: 650–659



Xu Z J, Lin H, Ma D R, Wang J Y, Xu H, Zhao M H, Chen W F. Research and application of the panicle type improved theory and technology in northern japonica rice. J Shenyang Agric Univ, 2012, 43: 650–659 (in Chinese with English abstract)



[3]Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, Swapan D, Ishiyama K, Saito T, Kobayashi M, Khush G S, Kitano H, Matsuoka M. Green revolution: a mutant gibberellin-synthesis gene in rice. Nature, 2002, 416: 701–702



[4]JiaoY Q, Wang Y H, Xue D W, Wang J, Yan M X, Liu G F, Dong G J, Zeng D L, Lu Z F, Zhu X D, Qian Q, Li J Y. Regulation of OsSPL14 by OsmiR156 defines ideal plant architecture in rice. Nat Genet, 2010, 42: 541–544



[5]Kotaro M, Mayuko I, Atsushi M, Song X J, Midori I, Kenji A, Makoto M, Hidemi K, Motoyuki A. OsSPL14 promotes panicle branching and higher grain productivity in rice. Nature Genet, 2010, 42: 545–549



[6]Li X Y, Qian Q, Fu Z M, Wang Y H, Xiong G S, Zeng D L, Wang X Q, Liu X F, Teng S, Hiroshi F, Yuan M, Luo D, Han B, Li J Y. Control of tillering in rice. Nature, 2003, 422: 618–621



[7]Qi J, Qian Q, Bu Q Y, Li S Y, Chen Q, Sun J Q, Liang W X, ZhouY H, Chu C C, Li X G, Ren F G, Palme K, Zhao B R, Chen J F, Chen M S, Li C Y. Mutation of rice narrow leaf1 gene, which encodes a novel protein, affects vein patterning and polar auxin transport. Plant Physiol, 2008, 147: 1947–1959



[8]Gao Z Y, Qian Q, Liu X H, Yan M X, Feng Q, Dong G J, Liu J, Han B. Dwarf 88, a novel putative esterase gene affecting architecture of rice plant . Plant Mol Biol, 2009, 71: 265–276



[9]Tomotsugu A, Mikihisa U, Shinji I, Atsushi H, Masahiko M, Shinjiro Y, Junko K. D14, a Strigolactone-insensitive mutant of rice, shows an accelerated outgrowth of tillers. Plant Cell Physiol, 2009, 50: 1416–1424



[10]Liu W Z, Wu C, Fu Y P, Hu G C, Si H M, Zhu L, Luan W J, He Z Q, Sun Z X. Identification and characterization of HTD2: a novel gene negatively regulating tiller bud outgrowth in rice. Planta, 2009, 230: 649–658



[11]Zhou F, Lin Q B, Zhu L H, Ren Y L, Zhou K N, Nitzan S, Wu F Q, Mao H B, Dong W, Gan L, Ma W W, Gao H, Chen J, Yang C, Wang D, Tan J J, Zhang X, Guo X P, Wang J L, Jiang L, Liu X, Chen W Q, Chu J F, Yan C Y, Kotomi U, Shinsaku I, Tadao A, Cheng Z J, Wang J, Lei C L, Zhai H Q, Wu C Y, Wang H Y, Zheng N, Wan J M. D14-SCFD3-dependent degradation of D53 regulates strigolactone signaling. Nature, 2013, 504: 406–410



[12]Jiang L, Liu X, Xiong G S, Liu H H, Chen F L, Wang L, Meng X B, Liu G F, Yu H, Yuan Y D, Yi W, Zhao L H, Ma H L, He Y Z, Wu Z S, Karsten M, Qian Q, Eric X, Wang Y H, Li J Y. DWARF 53 acts as a repressor of strigolactone signalling in rice. Nature, 2013, 504: 401–405



[13]韩龙植, 魏兴华. 水稻种质资源描述规范和数据标准. 北京: 中国农业出版社, 2006. pp 66–73



Han Z L, Wei X H. Rice Germplasm Description Specifications and Data Standards. Beijing: China Agriculture Press, 2006. pp 66–73



[14]Lu B R, Cai X X, Jin X. Efficient indica and japonica rice identification based on the InDel molecular method: Its implication in rice breeding and evolutionary research. Prog Nat Sci, 2009, 19: 1241–1252



[15]Zhao X Q, Wu W R. Construction of a genetic map based on ILP markers in rice. Hereditas (Beijing), 2008, 30: 225–230



[16]Lander E S, Green P, Abrahamson J, Barlow A, Daly M J, Lincoln S E. Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1987, 1: 174–181 



[17]Wang J K, Li H H, Zhang L Y, Li C H,Meng L. QTL ICI Mapping V3.0. Beijing, China: Institute of Crop Science of Chinese Academy of Agricultural Sciences, 2011. http://www.isbreeding.net/



[18]Voorrips R E. MapChart: Software for the graphical presentation of linkage maps and QTLs. J Hered, 2002, 93: 77–78



[19]McCouch S R, Cho Y G, Yano M, Paul E, Blinstrub M. Report on QTL nomenclature. Rice Genet Newsl, 1997, 14: 11–13



[20]李仕贵, 何平, 王玉平, 黎汉云, 周开达, 陈英, 朱立煌. 水稻剑叶性状的遗传分析和基因定位. 作物学报, 2000, 26: 261–265



Li S G, He P, Wang Y P, Li H Y, Zhou K D, Chen Y, Zhu L H. Genetic analysis and gene mapping of the leaf traits in rice (Oryza sativa L.). Acta Agron Sin, 2000, 26: 261–265 (in Chinese with English abstract)



[21]岳兵, 薛为亚, 罗利军, 邢永忠. 水稻剑叶部分形态生理特性QTL分析以及它们与产量、产量性状的关系. 遗传学报, 2006, 33: 824-832



Yue B, Xue W Y, Luo L J, Xing Y Z. QTL Analysis for flag leaf characteristics and their relationships with yield and yield traits in rice. J Genet Genomics, 2006, 33: 824–832 (in Chinese with English abstract)



[22]徐建军, 赵强, 赵元凤, 朱磊, 徐辰武, 顾铭洪, 韩斌, 梁国华. 利用重测序的水稻染色体片段代换系群体定位剑叶形态QTL. 中国水稻科学, 2011, 25: 483–487



Xu J J, Zhao Q, Zhao Y F, Zhu L, Xu C W, Gu M H, Han B, Liang G H. Mapping of QTLs for flag leaf shape using whole-genome re-sequenced chromosome segment substitution lines in rice. Chin J Rice Sci, 2011, 25: 483–487 (in Chinese with English abstract)



[23]王一平, 曾建平, 郭龙彪, 邢永忠, 徐才国, 梅捍卫, 应存山, 罗利军. 水稻顶部三叶与穗重的关系及其QTL分析. 中国水稻科学, 2005, 19: 13–20



Wang Y P, Zeng J P, Guo L B, Xing Y Z, Xu G C, Mei H W, Ying C S, Luo L J. QTL and correlation analysis on characters of top three leaves and panicle weight in rice (Oryza sativa L.). Chin J Rice Sci, 2005, 19: 13–20 (in Chinese with English abstract)



[24]彭茂民, 杨国华, 张菁晶, 安保光, 李阳生. 不同遗传背景下水稻剑叶形态性状的QTL分析. 中国水稻科学, 2007, 21: 247–252



Peng M M, Yang G H, Zhang J J, An B G, Li Y S. QTL Analysis for flag leaf morphological traits in rice (Oryza sativa L.) under different genetic backgrounds. Chin J Rice Sci, 2007, 21: 247–252 (in Chinese with English abstract)



[25]姜树坤, 张喜娟, 黄成, 邢亚南, 郑旭, 徐正进, 陈温福. 基于粳稻F2和F2:6群体的连锁图谱及剑叶性状QTL比较分析. 中国水稻科学, 2010, 24: 372–378



Jiang S K, Zhang X J, Huang C, Xing Y N, Zheng X, Xu Z J, Chen W F. Comparison of genetic linkage map and QTLs controlling flag leaf traits based on F2 and F2:6 populations derived from japonica rice. Chin J Rice Sci, 2010, 24: 372–378 (in Chinese with English abstract)



[26]刘进, 姚晓云, 李清, 张宇, 任春元, 王嘉宇, 徐正进. 水稻叶片性状QTL分析. 华北农学报, 2012, 27(5): 86–90



Liu J, Yao X Y, Li Q, Zhang Y, Ren C Y, Wang J Y, Xu Z J. QTL Analysis for the leaf traits in rice. Acta Agric Boreali-Sin, 2012, 27(5): 86–90 (in Chinese with English abstract)



[27]Hittalmani S, Shashidhar H E, Bagali P G, Huang N, Sidhu J S, Singh V P, Khush G S. Molecular mapping of quantitative trait loci for plant growth, yield and yield related traits across three diverse locations in a doubled haploid rice population. Euphytica, 2002, 125: 207–214



[28]方萍, 季天委, 陶勤南, 吴平. 两种供氮水平下水稻穗长QTLs的检测. 中国水稻科学, 2002, 16: 176–178



Fang P, Ji T W, Tao Q N, Wu P. Detecting QTLs for rice panicle length under two nitrogen levels. Chin J Rice Sci, 2002, 16: 176–178 (in Chinese with English abstract)



[29]杜景红, 樊叶杨, 王磊, 庄杰云. 应用剩余杂合体衍生的近等基因系分解水稻产量性状QTL. 中国水稻科学, 2008, 22: 1–7



Du J H, Fan Y Y, Wang L, Zhuang J Y. Dissection of QTLs for yield traits by using near isogenic lines derived from residual heterozygous lines in rice. Chin J Rice Sci, 2008, 22: 1–7 (in Chinese with English abstract)



[30]Yan C J, Zhou J H, Yan S, Chen F, Yeboah M, Tang S Z, Liang G H, Gu M H. Identification and characterization o f a major QTL responsible for erect panicle trait in japonica rice (Oryza sativa L.). Theor Appl Genet, 2007, 115: 1093–1100



[31]Wang J Y, Nakazaki T, Chen S Q, Chen W F, Saito H, Tsukiyama T, Okumoto Y, Xu Z J, Tanisaka T. Identification and characterization of the erect-pose panicle gene EP conferring high grain yield in rice (Oryza sativa L.). Theor Appl Genet, 2009, 119: 85–91



[32]Huang X Z, Qian Q, Liu Z B, Sun H Y, He S Y, Luo D, Xia G M, Chu C C, Li J Y, Fu X D. Natural variation at the DEP1 locus enhances grain yield in rice. Nat Genet, 2009, 41: 494–497

[1] LUO Lan, LEI Li-Xia, LIU Jin, ZHANG Rui-Hua, JIN Gui-Xiu, CUI Di, LI Mao-Mao, MA Xiao-Ding, ZHAO Zheng-Wu, HAN Long-Zhi. Mapping QTLs for yield-related traits using chromosome segment substitution lines of Dongxiang common wild rice (Oryza rufipogon Griff.) and Nipponbare (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2021, 47(7): 1391-1401.
[2] ZHANG Xiao-Qiong, WANG Xiao-Wen, TIAN Wei-Jiang, ZHANG Xiao-Bo, Sun Ying, LI Yang-Yang, Xie Jia, HE Guang-Hua,SANG Xian-Chun. LAZY1 Regulates the Development of Rice Leaf Angle through BR Pathway [J]. Acta Agron Sin, 2017, 43(12): 1767-1773.
[3] ZHONG Jie,WEN Pei-Zheng,SUN Zhi-Guang,XIAO Shi-Zhuo,HU Jin-Long,ZHANG Le,JIANG Ling,CHENG Xia-Nian,LIU Yu-Qiang,WAN Jian-Min. Identification of QTLs Conferring Small Brown Planthopper Resistance in Rice (Oryza sativa L.) Using MR1523/Suyunuo F2:3 Population [J]. Acta Agron Sin, 2017, 43(11): 1596-1602.
[4] ZHOU Ke,LI Yan,WANG Shi-Ming,CUI Guo-Qing,YANG Zheng-Lin,HE Guang-Hua,LING Ying-Hua,ZHAO Fang-Ming. Identification of Rice Chromosome Segment Substitution Line Z519 with Purple Sheath and Candidate Gene Analysis of PSH1 [J]. Acta Agron Sin, 2017, 43(07): 974-982.
[5] XIAO Yan-Hua**, CHEN Xin-Long**, DU Dan, XING Ya-Di, ZHANG Tian-Quan, ZHU Mao-Di,LIU Ming-Ming,ZHU Xiao-Yan, SANG Xian-Chun,HE Guang-Hua*. Identification and Gene Mapping of Starch Accumulation and Early Senescence Leaf Mutant esl9 in Rice [J]. Acta Agron Sin, 2017, 43(04): 473-482.
[6] LI Chuang, LIU Cheng-Chen, ZHANG Chang-Quan, ZHU Ji-Hui, XU Xiao-Ying, ZHAO Fu-Wei,HUANG Shao-Wen, JIN Yin-Gen,LIU Qiao-Quan. Genetic Diversity of ALK Gene and Its Association with Grain Gelatinization Temperature in Currently Cultivated Rice Landraces from Hani’s Terraced Fields in Yunnan Province [J]. Acta Agron Sin, 2017, 43(03): 343-353.
[7] YANG Bo,XIA Min, ZHANG Xiao-Bo,WANG Xiao-Wen,ZHU Xiao-Yan,HE Pei-Long,HE Guang-Hua,SANG Xian-Chun*. Identification and Gene Mapping of an Early Senescent Leaf Mutant esl6 in Oryza sativa L. [J]. Acta Agron Sin, 2016, 42(07): 976-983.
[8] HE Ni-Qing,LIU Zhou,ZHANG Long,BAI Su-Yang,TIAN Yun-Lu,JIANG Ling,WAN Jian-Min. Genetic Analysis of a New Yellow-green Leaf Mutant and Fine-mapping of Mutant Gene in Rice [J]. Acta Agron Sin, 2015, 41(08): 1155-1163.
[9] FENG Ping**,XING Ya-Di**,LIU Song,GUO Shuang,ZHU Mei-Dan,LOU Qi-Jin,SANG Xian-Chun,HE Guang-Hua,WANG Nan. Characterization and Gene Mapping of Rolled Leaf Mutant 28 (rl28) in Rice (Oryza sativa L.) [J]. Acta Agron Sin, 2015, 41(08): 1164-1171.
[10] ZHANG Tian-Quan,GUO Shuang,XING Ya-Di,DU Dan,SANG Xian-Chun,LING Ying-Hua,HE Guang-Hua. Molecular Mapping of a New Yellow Green Leaf Gene YGL9 in Rice (Oryza sativa L.) [J]. Acta Agron Sin, 2015, 41(07): 989-997.
[11] ZHONG Zhen-Quan,LUO Wen-Long,LIU Yong-Zhu,WANG Hui,CHEN Zhi-Qiang,GUO Tao. Characterization of a Novel Spotted Leaf Mutant spl32 and Mapping of Spl32(t) Gene in Rice (Oryza sativa) [J]. Acta Agron Sin, 2015, 41(06): 861-871.
[12] TAN Yan-Ning,SUN Xue-Wu,YUAN Ding-Yang,SUN Zhi-Zhong,YU Dong,HE Qiang,DUAN Mei-Juan,DENG Hua-Feng,YUAN Long-Ping. Identification and Fine Mapping of Green-Revertible Chlorina Gene grc2 in Rice (Oryza sativa L.) [J]. Acta Agron Sin, 2015, 41(06): 831-837.
[13] WANG Yan-Zheng,WANG Xiao-Jing,LI Yuan,XU Hai,WANG Jia-Yu,ZHAO Ming-Hui,TANG Liang,MA Dian-Rong,XU Zheng-Jin,CHEN Wen-Fu. Analysis of Yield and Quality Traits and the Relationship between Them in Japonica Rice in the Northern China [J]. Acta Agron Sin, 2015, 41(06): 910-918.
[14] WANG Xing-Chun,WANG Min,JI Zhi-Juan,CHEN Zhao,LIU Wen-Zhen,HAN Yuan-Huai,YANG Chang-Deng. Functional Characterization of the Glycoside Hydrolase Encoding Gene OsBE1 during Chloroplast Development in Oryza sativa [J]. Acta Agron Sin, 2014, 40(12): 2090-2097.
[15] WANG Bao-Xiang,HU Jin-Long,SUN Zhi-Guang,SONG Zhao-Qiang,LU Bai-Guan,ZHOU Zhen-Ling,FAN Ji-Wei,QIN De-Rong,LIU Yu-Qiang,JIANG Ling,XU Da-Yong,WAN Jian-Min. An Evaluation System for Rice Black-Streaked Dwarf Virus Disease and Screening for Resistant Rice Germplasm [J]. Acta Agron Sin, 2014, 40(09): 1521-1530.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!