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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (12): 2218-2224.doi: 10.3724/SP.J.1006.2009.02218

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation and Characterization of IbNPR1 Gene from Sweet Potato（Ipomoea batatas）

CHEN Guan-Shui1,ZHOU Yi-Fei2,LIN Sheng1,ZHANG Zheng1,PAN Da-Ren1,*   

  1. 1College of Life Science，Fujian Agriculture and Forestry University，Fuzhou 35002，China，College of Crop Science，Fujian Agriculture and Forestry University，Fuzhou 350002，China
  • Received:2008-12-31 Revised:2009-06-25 Online:2009-12-10 Published:2009-09-10
  • Contact: PAN Da-Ren; E-mail: pandaren@yahoo.com.cn; Tel: +86 59183798876
  • Supported by:

    This study was supported by Fujian Province Natural Science Foundation (2006J0059) and Youth Foundation of Fujian Agriculture and Forestry University(08B12)

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Abstract:

NPR1 (non-expressor of pathogenesis-related genes 1) protein is a key regulator of salicylic acid (SA)-mediated gene epression in systemic acquired resistance (SAR). By using homologous cloning and RACE (rapid amplification of cDNA ends) techniques, a full-length cDNA of IbNPR1 (Ipomoea batatas non-expressor of pathogenesis-related genes 1) was isolated from sweet potato var. Qingnong 2. The full length cDNA was 2 353 bp, including an ORF (open reading frame) putatively encoding a polypeptide of 586 amino acids residues with a predicted molecular mass of 64.851 kD. The deduced amino acid sequence shared structural features with known NPR1 (-like) proteins: ankyrin repeat and BTB/POZ. Furthermore, phylogenetic analysis showed IbNPR1 had the closest association with LeNPR1 from Lycopersicon esculentum. Southern-blot analysis revealed that the IbNPR1 belonged to low-copy gene family in sweet potato. Semi-quantitative RT-PCR analysis indicated that IbNPR1 was constitutively expressed in roots, stems and leaves. In addition, IbNPR1 could be induced by salicylic acid. The results suggest that IbNPR1 plays an important role in the response to pathogen infections in sweet potato.

Key words: Ipomoea batatas, IbNPR1 gene, Disease resistance, RACE

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