Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (12): 2234-2242.doi: 10.3724/SP.J.1006.2009.02234


Fertilization Process in Sorghum and Its Performance Time for Each Stage

LI Ran-Li1,SHEN Jia-Heng1,*,JIA Yuan1,LI Wei1,WANG Li-Ming2   

  1. 1College of Life Sciences and Technology,Harbin Normal University,Harbin 150025,China;2Institute of Crop Breeding,Heilongjiang Academy of Agricultural Sciences,Harbin 150080,China
  • Received:2009-04-24 Revised:2009-08-21 Online:2009-12-10 Published:2009-10-13
  • Contact: SHEN JIa-Heng,E-mail:hsdshenjiaheng@yahoo.com.cn;Tel:0451-88068652


Sorghum (Sorghum bicolor L. Moench) grows widely in the world as the fifth biggest crop in use for food, feed and raw material in brewing industry. Great achievements have been taken in routine breeding, and also in apomixis in sorghum. Reports on sorghum embryology mainly focused on the morphological structure and development of flower, megasporogenesis, microsporogensis, megagametophyte and so on. The objective of this study was to explore the fertiligation process of sorghum by routine paraffin-embedded section technique to offer some information for its genetic breeding, gene engineering and reproductive biology. The results showed that pollen grains germinated immediately when they fell on stigma in self-pollinated sorghum, then pollen tubes grew into the intercellular space of branched structure of penniform stigma, entering and penetrating ovary, and growing into micropyle. Pollen tube entered and destroyed one synergid and released successive round staff between egg and central cells. One sperm nucleus entered egg and the other entered central cell. Male and female nucleoli were fused at last. The performance time was as follows: pollen tube grew into micropyle and entered one synergid to release two sperms at 0.5–3 h after flowering; fusion of sperm-polar nucleus and egg-sperm nucleus took place at about 1–3 h after flowering; primary endosperm nucleus divided at about 4 h after flowering; zygote divided at 14 h after flowering; the dormancy stage of zygote was about 4–14 h after flowering, which is the proper time for transgenic operation with pollen-tube pathway technology and other physic-chemical mutagenesis.

Key words: Sorghum bicolor(L.) Moench, Pathway of pollen tube growth, Fertilization, Duration, Zygophase

[1] Lu S-W(陆时万), Xu X-S(徐祥生), Shen M-J(沈敏健). Botany (植物学), 2nd edn. Beijing: Higher Education Press, 1999. p 345 (in Chinese)

[2] Zhao F-W(赵风悟), Li H-M(李惠敏), Liu D-C(刘冬成), Zhang A-M(张爱民). Germplasm innovation by hybridization between upland rice and sorghum. J Nucl Agric Sci (核农学通报), 2006, 20(2): 106-109 (in Chinese with English abstract)

[3] Zhan Q-W(詹秋文), Qian Z-Q(钱章强). Heterosis utilization of hybrid between sorghum and sudangrass. Acta Agron Sin (作物学报), 2004, 30(1): 73-77 (in Chinese with English abstract)

[4] Ping J-A(平俊爱), Zhang F-Y(张福耀), Cui G-M(崔贵梅), Cheng Q-J(程庆军), Du Z-H(杜志宏), Zhang Y-X(张义贤). A study on the properties of autonomous seed setting and embryology in sorghum apomictic line 2083. Acta Agron Sin (作物学报), 2004, 30(7): 714-718 (in Chinese with English abstract)

[5] Wu S-B(吴树彪), Zhang F-Y(张福耀). Embryological study on apomixisina sorghum line SSA-1. Aata Bot Sin (植物学报), 1994, 36(11): 833-837 (in Chinese with English abstract)

[6] Schertz K F, Bashaw E C. Apomixis in Sorghum bicolor (L.) Moench. Science, 1970, 170: 338-339

[7] Li Q-L(李秋莉), Xu A-J(徐爱菊). Study on the course of formation of male and female gametophyte in Sorghum vulgare Pers. J Liaoning Nor Univ (辽宁师范大学学报), 1997, 20(2): 151-154 (in Chinese with English abstract)

[8] Lu Q-S(卢庆善). Sorghum (高粱学). Beijing: China Agriculture Press, 1999. pp 67-73 (in Chinese)

[9] Doggett H. Sorghum. London: Longman, 1972. pp 66-68

[10] Li Y-H(李扬汉). Morphology and Anatomy of Gamineae Crop (禾本科作物的形态与解剖). Shanghai: Shanghai Science and Technology Press, 1979. p 377 (in Chinese)

[11] Wang Y-J(王艳杰), Shen J-H(申家恒). Probing into cytological embryology mechanism of pollen-tube pathway transgenic technology. Acta Bot Boreal-Occident Sin (西北植物学报), 2006, 26(3): 628-634 (in Chinese with English abstract)

[12] Shen J-H(申家恒), Li H-R(李惠蓉), Li Y-F(李玉芬), Yin H(殷华), Lian Y-Q(连永权). Cytologic observations on the double fertilization in maize. Aata Bot Sin(植物学报), 1987, 29(5): 480-485 (in Chinese with English abstract)

[13] Wu S-X(吴素萱), Cai Q-G(蔡起贵). Cytologic observations on the double fertilization in Oryza. Aata Bot Sin (植物学报), 1965, 13(2): 114-121 (in Chinese with English abstract)

[14] Shen J-H(申家恒), Wang P(王谱), Li H-R(李惠蓉), Yin H(殷华). On the process of fertilization in spring wheat and its duration of each stage. Acta Agron Sin (作物学报), 1983, 9(2): 79-83 (in Chinese with English abstract)

[15] Zhou C(周嫦), Yang H-Y(杨弘远). Embryologic Studies on Several Questions during Fertilization in Wheat. Genetics Collected Papers (遗传学集刊), 1964, 4: 36-49 (in Chinese)
Zhang B-L(张伯林), Tao S-H(陶舜华). Observation on fertilization and zygote duration in sorghum. Appl Atom Energy Agric (原子能农业应用), 1981, 3: 1-6 (in Chinese)
[1] XIAO Jian, CHEN Si-Yu, SUN Yan, YANG Shang-Dong, TAN Hong-Wei. Characteristics of endophytic bacterial community structure in roots of sugarcane under different fertilizer applications [J]. Acta Agronomica Sinica, 2022, 48(5): 1222-1234.
[2] QIN Qin, TAO You-Feng, HUANG Bang-Chao, LI Hui, GAO Yun-Tian, ZHONG Xiao-Yuan, ZHOU Zhong-Lin, ZHU Li, LEI Xiao-Long, FENG Sheng-Qiang, WANG Xu, REN Wan-Jun. Characteristics of panicle stem growth and flowering period of the parents of hybrid rice in machine-transplanted seed production [J]. Acta Agronomica Sinica, 2022, 48(4): 988-1004.
[3] FANG Yan-Jie, ZHANG Xu-Cheng, HOU Hui-Zhi, YU Xian-Feng, WANG Hong-Li, MA Yi-Fan, ZHANG Guo-Ping, LEI Kang-Ning. Effects of whole soil-plastic mulching system and fertilization rates on water consumption characteristics and yield of tartary buckwheat in arid land [J]. Acta Agronomica Sinica, 2021, 47(6): 1149-1161.
[4] LIU Yan-Lan, GUO Xian-Shi, ZHANG Xu-Cheng, MA Ming-Sheng, WANG Hong-Kang. Effects of planting density and fertilization on dry matter accumulation, yield and water-fertilizer utilization of dryland potato [J]. Acta Agronomica Sinica, 2021, 47(2): 320-331.
[5] HUANG Heng, JIANG Heng-Xin, LIU Guang-Ming, YUAN Jia-Qi, WANG Yuan, ZHAO Can, WANG Wei-Ling, HUO Zhong-Yang, XU Ke, DAI Qi-Gen, ZHANG Hong-Cheng, LI De-Jian, LIU Guo-Lin. Effects of side deep placement of nitrogen on rice yield and nitrogen use efficiency [J]. Acta Agronomica Sinica, 2021, 47(11): 2232-2249.
[6] HOU Hong-Qian,LIN Hong-Xin,LIU Xiu-Mei,JI Jian-Hua,LIU Yi-Ren,LAN Xian-Jin,LYU Zhen-Zhen,ZHOH Wei-Jun. Influence of long-term fertilizer application on chlorophyll fluorescence characteristics and grain yield of double cropping late rice [J]. Acta Agronomica Sinica, 2020, 46(02): 280-289.
[7] Yue-E LIU,Tian-Fang LYU,Jiu-Ran ZHAO,Rong-Huan WANG,Tian-Jun XU,Chuan-Yong CHEN,Yi-Tian ZHANG,Yuan-Dong WANG,Xiu-Zhi LIU. Silking duration characteristics in different maize hybrids and its response to sowing date [J]. Acta Agronomica Sinica, 2019, 45(2): 310-315.
[8] Ying-Bin ZOU,Min HUANG. Opportunities and Challenges for Crop Production in China during the Transition Period [J]. Acta Agronomica Sinica, 2018, 44(6): 791-795.
[9] CHEN Hong-Fei, PANG Xiao-Min, ZHANG Ren, ZHANG Zhi-Xing, XU Qian-Hua, FANG Chang-Xun, LI Jing-Yong, LIN Wen-Xiong . Effects of Different Irrigation and Fertilizer Application Regimes on Soil Enzyme Activities and Microbial Functional Diversity in Rhizosphere of Ratooning Rice [J]. Acta Agron Sin, 2017, 43(10): 1507-1517.
[10] ZHANG Jian-Jun*,FAN Ting-Lu,ZHAO Gang,DANG Yi,WANG Lei,LI Shang-Zhong. Yield and Water Use Efficiency of Winter Wheat in Response to Long-Term Application of Organic fertilizer from Different Nitrogen Resources Replacing Partial Chemical Nitrogen in Dry Land of Eastern Gansu Province [J]. Acta Agron Sin, 2017, 43(07): 1077-1086.
[11] CHEN Dan-Mei,YUAN Ling,HUANG Jian-Guo,JI Jian-Hua,HOU Hong-Qian,LIU Yi-Ren. Influence of Long-term Fertilizations on Nutrients and Fungal Communities in Typical Paddy Soil of South China [J]. Acta Agron Sin, 2017, 43(02): 286-295.
[12] ZHANG Yu-Jiao,LI Jun,GUO Zheng,YUE Zhi-Fang. Simulating Wheat Yield and Soil Moisture under Alternative No-tillage and Subsoil Tillage in Response to Fertilization Levels in Weibei Highlands [J]. Acta Agron Sin, 2015, 41(11): 1726-1739.
[13] MENG Tian-Yao,XU Jun-Wei,SHAO Zi-Bin,GE Meng-Jie2,ZHANG Hong-Cheng,WEI Hai-Yan,DAI Qi-Gen,HUO Zhong-Yang,XU Ke,GUO Bao-Wei,JING Pei-Pei. Advantages and Their Formation Characteristics of the Highest Population Productivity of Nitrogen Fertilization in Japonica/Indica Hybrid Rice of Yongyou Series [J]. Acta Agron Sin, 2015, 41(11): 1711-1725.
[14] ZHANG Ya-Jie,LI Jing,PENG Hong-Kun,CHEN Xiu-Bin,ZHENG Hong-Yu,CHEN Sheng-Bei,LIU An-Guo,HU Li-Yong. Dynamic Simulation Model for Growth Duration of Rapeseed (Brassica napus) [J]. Acta Agron Sin, 2015, 41(05): 766-777.
[15] SUN De-Xiang,MA Dong-Yun*,WANG Chen-Yang,LI Yao-Guang,LIU Wei-Xing,LI Qiu-Xia,FENG Wei,GUO Tian-Cai*. Effects of Irrigation and Nitrogen on Antioxidant Contents in Yumai 49-198 Grains [J]. Acta Agron Sin, 2014, 40(11): 2046-2051.
Full text



No Suggested Reading articles found!