花生白藜芦醇合成酶基因PNRS1的克隆及其在原核中的表达

doi:10.3724/SP.J.1006.2010.00341

Abstract

Abstract:

Resveratrol synthetic enzyme (RS) is a key and necessary enzyme that plays important role in resveratrol synthesis pathway. To uncover and characterize the function of the RS in plant development process, we isolated a RS gene PNRS1 (FM955393) by RT-PCR using total RNA of peanut seed as template, and the gene PNRS1 was expressed in E. coli prokaryote for further analysis. The results showed that the PNRS1 had a 1 170 bp open reading frame encoding 389 amino acid polypeptide, and exhibiting high similarities with other members of RS genes family. Expression pattern analysis indicated that the PNRS1 was specially expressed in peanut root, and could be induced by UV-B treatment. The recombinant PNRS1 protein product with the molecular weight about 46 kD could also be detected in E. coli protein expression system. These results suggested that the PNRS1 was a mumber of RS family with peculiar expression patterns compared with other ones. As protein is the functional executor of a gene, the protein product of PNRS1 finally will take effect in the later processes of plant development, and shed light on the stress-resistant breeding and cultivation.

Key words: Peamut, Resveratrol synthetic enzyme(RS), Expression pattern, Prokaryotic expression system, Fusion protein

HAN Jing-Jing,LIU Wei,BI Yu-Ping. Molecular Cloning of Peanut Resveratrol Synthetic Enzyme 1(PNRS1) and its Expression in Prokaryote[J].Acta Agron Sin, 2010, 36(2): 341-346.

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Molecular Cloning of Peanut Resveratrol Synthetic Enzyme 1(PNRS1) and its Expression in Prokaryote

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