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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (08): 1318-1323.doi: 10.3724/SP.J.1006.2010.01318

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping QTLs for Lint Percentage and Seed Index by Using Gossypium barbadense Chromosome Segment Introgression Lines

 ZHU Ya-Juan,WANG Peng**,GUO Wang-Zhen, ZHANG Tian-Zhen*   

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2009-11-02 Revised:2010-04-20 Online:2010-08-12 Published:2010-05-20
  • Contact: ZHANG Tian-Zhen, E-mail: cotton@njau.edu.cn; Fax: 025-84395307

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Abstract: Seed index (SI) and lint percentage (LP) are very important components, and many researchers have done abundant investigation on them by using different populations. Chromosome segment introgression lines (CSIL), different from the recurrent parent in introgressed segments, are the ideal materials for QTL research and the core of the breeding by design. The F2 and F2:3 populations were developed from CSILs-IL-15-5 and IL-15-5-1, and used in mapping QTLs for lint percentage and seed index with SSR markers. The SI and LP characteristics of the 774 F2 individuals and F2:3 families were analyzed by composite interval mapping (CIM), and one QTL was detected for SI and two QTLs for LP. qSI-15-1 was located between NAU2814 and NAU3040 with confident genetic distance of 6.70 cM in the F2 population and between NAU3040 and JESPR152 with confident genetic distance of 5.70 cM in the F2:3 population. qLP-15-1 detected in the F2 and F2:3 populations was located between NAU3040 and JESPR152 with confident genetic distance of 5.40 cM and 3.20 cM respectively; qLP-15-2 was mapped between NAU5302 and NAU2901 with confident genetic distance of 0.08 cM. In conclusion, the QTLs for yield components in cotton can be mapped using CSILs and provide a base for breeding by design.

Key words: Chromosome segment introgression lines, Cotton, Lint percentage, Seed index, QTL

[1]Jiang C X, Wright R J, El-Zik K M, Paterson A H. Polyploid formation created unique avenues for response to selection in Gossypium (cotton)
[J].Proc Natl Acad Sci USA
[2]Wu M Q, Zhang X L, Nie Y C, He D H. Localization of QTLs for yield and fiber quality traits of tetraploid cotton cultivar. Acta Genet Sin, 2003, 30: 443-452
[3]Zhang J F, Percy R. Improving upland cotton by introducing desirable genes from Pima Cotton. In: World Cotton Research Conf. 4, Lubbock, Texas, USA, Sept. 10-14, 2007. p 1901
[4]Ebitani T, Takeuchi Y, Nonoue Y, Yamamoto T, Takeuchi K, Yano M. Construction and evaluation of chromosome segment substitution lines carrying overlapping chromosome segments of indica rice cultivar ‘Kasalath’ in a genetic background of japonica elite cultivar ‘Koshihikari’
[J].Breed Sci
[5]Lin H X, Liang Z W, SasakI T, Yano M. Fine mapping and charac-
[6]terization of quantitative trait loci Hd4 and Hd5 controlling heading date in rice.
[J]. Breed Sci
[7]Wu X-R(吴新儒), Liu S-B(刘树兵), Liu A-F(刘爱峰), Deng S-M(邓世民), Wang H-G(王洪刚), Zhou R-H(周荣华). Deve-
[8]lopment of near isogenic introgression lines (NIILs) of agronomic important traits in wheat. J Triticeae Crops (麦类作物学报), 2007, 27(4): 583-588 (in Chinese with English abstract)
[9]Wang L-Q(王立秋), Zhao Y-F(赵永锋), Xue Y-D(薛亚东), Zhang Z-X(张祖新), Zheng Y-L(郑用琏), Chen J-T(陈景堂). Development and evaluation of two link-up single segment introgression lines (SSILs) in Zea mays. Acta Agron Sin (作物学报), 2007, 33(4): 663-668 (in Chinese with English abstract)
[10]Paterson A H, Brubaker C L, Wendel J F. A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP and PCR analysis. Plant Mol Biol Rep, 1993, 11: 122-127
[11]Zhang J(张军), Wu Y-T(武耀廷), Guo W-Z(郭旺珍), Zhang T-Z(张天真). Fast screening of microsatellite markers in cotton with PAGEP silver staining. Acta Gossypii Sin (棉花学报), 2000, 12(5): 267-269 (in Chinese with English abstract)
[12]Guo W Z, Cai C P, Wang C B, Zhao L, Wang L, Zhang T Z. A preliminary analysis of genome structure and composition in Gossypium hirsutum
[J]. BMC Genomics
[13]Van Ooijen J W, Voorrips R E. JoinMap Version 3.0: Software for the Calculation of Genetic Linkage Maps. Wageningen: CPRO- DLO, 2001
[14]Wang S, Basten C J, Zeng Z B. Windows QTL Cartographer 2.0 CP. Department of Statistics, North Carolina State University, Raleigh, NC, 2004
[15]Zeng Z B. Precision mapping of quantitative trait loci. Genetics, 1994, 136: 1457-1468
[16]Lander E S, Kruglyak L. Genetic dissection of complex traits guidelines for interpreting and reporting linkage results
[J].Nat Genet
[17]Paterson A H, Saranga Y, Menz M, Jiang C X, Wright R J. QTL analysis of genotype × environment interactions affecting cotton fiber quality. Theor Appl Genet, 2003, 106: 384-396
[18]Voorips R E.Mapchart Version 2.0: Windows Software for the Graphical Presentation of the Linkage Maps and QTL. Wageningen, the Netherlands: Plant Research International, 2001
[19]McCouch S R, Cho Y G, Yano M, Paul E, Blinstrub M, Mori-
[20]shima H, Kinosita T. Report on QTL nomenclature. Rice Genet Newslett, 1997, 14: 11-13
[21]Shen X L, Guo W Z, Lu Q X, Zhu X F, Yuan Y L, Zhang T Z. Genetic mapping of quantitative trait loci for fiber quality and yield trait by RIL approach in upland cotton
[J]. Euphytica
[22]Li C-Q(李成奇), Guo W-Z(郭旺珍), Ma X-L(马晓玲), Zhang T-
[23]Z(张天真). Tagging and mapping of QTL for yield and its components in upland cotton (Gossypium hirsutum L.) population with varied lint percentage. Cotton Sci (棉花学报), 2008, 20(3): 163-169 (in Chinese with English abstract)
[24]Ulloa M, Meredith Jr W R. Genetic linkage map and QTL analysis of agronomic and fiber quality traits in an intraspecific population. J Cotton Sci, 2000, 4: 161-170
[25]Yin J-M(殷剑美), Wu Y-T(武耀廷), Zhang J(张军), Zhang T-Z(张天真), Guo W-Z(郭旺珍), Zhu X-F(朱协飞). Tagging and mapping of QTLs controlling lint yield and yield components in upland cotton (Gossypium hirsutum L.) using SSR and RAPD markers. Chin J Biotechnol (生物工程学报), 2002, 18(2): 162-166 (in Chinese with English abstract)
[26]Yamamoto T, Kuboki Y, Lin S Y, Sasaki T, Yano M. Fine mapping of quantitative trait loci Hd-1, Hd-2 and Hd-3, controlling heading date of rice, as single Mendelian factors
[J].Theor Appl Genet
[27]Peleman J D, vander Voort J R. Breeding by design
[J].Trends Plant Sci
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