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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (10): 1769-1776.doi: 10.3724/SP.J.1006.2010.01769


Accumulation Dynamics of Glutenin Subunits and Glutenin Polymer in Wheat Cultivars with Different Qualities during Grain Development

GAO Wen-Chuan,MA Meng,WANG Ai-Na,ZHAO Hui-Xian*   

  1. College of Life Science, Northwest A&F University, Yangling 712100, China
  • Received:2010-03-09 Revised:2010-05-29 Online:2010-10-12 Published:2010-08-04
  • Contact: ZHAO Hui-Xian,E-mail:hxzhao212@yahoo.com.cn,Tel:029-87062928

Abstract: The quality of wheat (Triticum aestivum L.) flour for end use depends on the visco-elastic properties of dough, which are influenced by quantity and quality of the gluten-forming storage proteins of wheat endosperm. Gliadin and glutenin are major components of gluten protein. Dough strength is determined by the capacity of glutenin subunits to form large polymers with different sizes, and the quantity and composition of glutenin polymer influence wheat dough properties. To date, there are few reports on accumulation dynamics of glutenin subunits and glutenin polymer in wheat cultivars with different qualities during grain development. In this study, three strong-gluten and four weak-gluten wheat cultivars with different HMW-GS compositions were used to analyze accumulation dynamics of glutenin subunits and glutenin polymer during grain development. The results showed that HMW-GS and LMW-GS of strong-gluten cultivars initially expressed at 9–12 d after flowering, which were earlier than those of weak-gluten cultivars (12–15 d after flowering). Expression time of glutenin subunits in wheat cultivars with strong-gluten was a little earlier than that in wheat cultivars with weak-gluten. The quantity of HMW-GS in each cultivar rapidly increased during the early period of grain-filling, and kept stable from 27 d after flowering to maturity, however, the quantity of LMW-GS accumulated slowly during the grain-filling period, reaching stable level at 30 d after flowering. In wheat cultivars with strong-gluten, TGP (total glutenin polymer) % and GMP (glutenin macropolymer) % rapidly increased at 12–21 d after flowering and slowly increased during 21–30 d after flowering, and then maintained at the stable level from 30 d after flowering to maturity. However, in weak-gluten cultivars, TGP% and GMP% rapidly increased at 12–24 d after flowing and decreased continuously from 27 d after flowering to maturity. The differences of expression profile and accumulation of glutenin subunits and glutenin polymer between wheat cultivars with strong-gluten and weak-gluten may be the key factors affecting strong-gluten or weak-gluten formation of wheat cultivars.

Key words: Triticum aestivum L., Glutenin subunit, Glutenin polymeric protein, Accumulation dynamics

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