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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (08): 1392-1402.doi: 10.3724/SP.J.1006.2014.01392


Cloning of Autophagy-Related Genes, ATG10s, in Wheat and Their Expression Characteristics Induced by Blumeria graminis f. sp. tritici

ZHANG Wei1,SUN Hong1,WEI Xiao-Jing1,XING Li-Ping2,WANG Hua-Zhong1,*   

  1. 1 School of Life Sciences, Tianjin Normal University / Tianjin Key Laboratory of Animals and Plants Resistance, Tianjin 300387, China; 2 National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2013-11-20 Revised:2014-04-16 Online:2014-08-12 Published:2014-06-03
  • Contact: 王华忠, E-mail: skywhz@mail.tjnu.edu.cn


Autophagy, a conserved eukaryotic cellular process functioning in material decomposition and nutrient recycling, is deeply involved in plant growth, development, and response to stresses. ATG10 is one of the key factors required in autophagosome formation. In this study, we identified three members (TaATG10a, TaATG10b, and TaATG10c) of the ATG10 family in common wheat 92R137/Yangmai 1587 induced by Blumeria graminis f. sp. tritici for 48 h, using homologous cloning technique. The three TaAGT10s shared high similarity with their counterparts from other model plants. Expression of TaATG10a or TaATG10b rescued the autophagy process in ATG10-defective yeast mutant, suggesting that both genes are functional homologues of yeast ATG10. TaATG10a and TaATG10b had similar gene models containing six exons and five introns, and both had two alternatively-spliced mRNA isoforms according to RT-PCR assay. TaATG10a- and TaATG10b-GFP fusion structures were located in cytosol of onion epidermal cells. The expression of TaATG10a and TaATG10b was induced by the infection of Blumeria graminis f. sp. tritici (Bgt), implying that TaATG10s and their involved autophagy process are implicated in the wheat immune response to Bgt. This implication is very complex because the regulated expression profiles of TaATG10a and TaATG10b are different between resistant and susceptible reactions, between different types of resistance gene-mediated immune reactions, and between susceptible reactions on different genetic backgrounds. Besides, exogenous phytohormones also modulated TaATG10a and TaATG10b expressions. The difference of Bgt immune reaction between the resistant and susceptible genotypes might result partially from the different response patterns of TaATG10sto exogenous salicylic acid, ethylene, or abscisic acid.

Key words: Triticum aestivum L., Autophagy-related genes, Powdery mildew

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