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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (08): 1403-1411.doi: 10.3724/SP.J.1006.2014.01403

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Rice Lateral Root Development and Its Impact Factors

LIU Da-Tong,JING Yan-Ping,CHEN Jing-Jing,YU Xu-Run,WANG Zhong*   

  1. College of Bioscience and Biotechnology, Yangzhou University / Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou 225009, China
  • Received:2014-01-08 Revised:2014-06-06 Online:2014-08-12 Published:2014-06-17

Abstract:

The adventitious roots and primary roots of Nipponbare, Yangdao 6were used to measure lateral root number and the growth increment by Image J software with scanning photograph. Morphological and structural changes of lateral root primodia were observed by applying Spurr resin embedding and semi-thin sectioning, and light microscopy. Impacts of the lateral root emergence on the parent root structure was observed by fluorescence microscopy and scanning electron microscopy. Treatments with plant hormones, metal ions, illumination, and cutting off shoots or root tips were set up to analyse their influence on lateral root development. Results showed that rice lateral root initiation occurred from the pericycle. The endodermis also participatedin the lateral root primodia formation and played protective as well as assistant roles in the process. The outward growth of lateral root primordia was realized by cell divisions of apical meristem and cell elongation at the base area. The cortex and epidermis structures of parent roots were also altered during the process of lateral root emergence. Exogenous IAA promoted the occurrence of lateral roots, but its high concentrations inhibited lateral root emergence. The number of lateral roots and growth of primary roots were significantly decreased when shoots were cut off. The apical dominance of root was lost when root tips were cut off, so that the growth of lateral roots and secondary lateral roots was accelerated. Unilateral illumination induced the increase of lateral roots in the light side. Abscisic acid at low concentrations promoted the formation of lateral roots. The formation of lateral roots and growth of primary root were promoted by Ca2+, while mostly inhibited by EDTA. The growth tendency of both adventitious roots and primary roots responsive to the treatments was basically consistent.

Key words: Rice, Lateral root development, Pericycle, Endodermis, IAA, Illumination

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