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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (03): 439-446.doi: 10.3724/SP.J.1006.2014.00439

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Vernalization Effects of Dominant Alleles Vrn-B1a and Vrn-B1b and Their Distributions in Cultivars from Yellow and Huai River Valleys Facultative Winter Wheat Zone

WANG Xuan,JU Li-Ping,LIU Fang-Jun,ZHANG Yu-Yu,ZHANG Fan,FU Xiao-Jie,FENG Yi,ZHANG Xiao-Ke*   

  1. College of Agronomy, Northwest A&F University, Yangling 712100, China
  • Received:2013-05-22 Revised:2013-10-22 Online:2014-03-12 Published:2014-01-16
  • Contact: 张晓科, E-mail: zhangxiaoke66@126.com

Abstract:

Vrn-B1 is a key gene controlling vernalization of wheat in the Yellow and Huai River Valleys Facultative Winter Wheat Zone of China. This study aimed to understand the allelic distribution at Vrn-B1 locus and their effects on heading date of wheat cultivars. To compare the vernalization responses of dominant alleles Vrn-B1a with Vrn-B1b on heading date, we planted F2 plants derived from the cross between Yumai 34 (Vrn-B1b genotype) and Wanmai 33 (Vrn-B1a genotype) in greenhouse (22±3°C and 16 h day/8 h night) with 4°C treatment for different periods (5–35 d). Besides, the Vrn-B1 allelic variation and distribution of 228 historic and current wheat cultivars were evaluated using molecular markers. The results showed that heading date of Vrn-B1a genotype was about two days later than that of Vrn-B1b genotype. Vernalization treatment at 4°C for 5–25 d obviously shortened the period from sowing to heading in genotype Vrn-B1a or Vrn-B1b; however, continuous vernalization had no effect to further accelerate heading. Therefore, 20–25 d is enough for vernalization in both genotypes. In the 228 cultivars, 214 carried the recessive vrn-B1 allele with the frequency of 93.9%, and the remaining 14 cultivars had dominantalleles on Vrn-B1 locus, including6 (2.6%) of Vrn-B1a genotype and 8 (3.5%) of Vrn-B1b genotype. The two dominant alleles on Vrn-B1 locus had different effects to accelerate heading in winter wheat, and can be used in wheat breeding programs to improve adaptability and cold tolerance in wheat.

Key words: Triticum aestivum L., Vernalization effect, Vrn-B1a, Vrn-B1b

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