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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (08): 1372-1377.doi: 10.3724/SP.J.1006.2011.01372

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Effect of Abscisic Acid Treatment on psbA Gene Expression in Two Wheat Cultivars during Grain Filling Stage under Drought Stress

WANG Yue-Xia1,SUO Biao2,ZHAO Teng-Fei1,QU Xiao-Fei1,YUAN Li-Gang1,ZHAO Xue-Juan1,ZHAO Hui-Jie1,*   

  1. 1 College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China; 2 College of Food Science & Technology, Henan Agricultural University, Zhengzhou 450002, China
  • Received:2011-02-28 Revised:2011-04-26 Online:2011-08-12 Published:2011-06-13
  • Contact: 赵会杰, E-mail: zhaohj303@163.com, Tel: 0371-63555319

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Abstract: Abscisic acid (ABA) is an important plant hormone and may be involved in drought resistance in crops. The effects of exogenous ABA application on wheat plant (Triticum aestivum L.) under drought stress were investigated. When two wheat cultivars, Yumai 949 and Shanmai 5 in grain filling stage were drought stressed by PEG for 72 h, the results showed a marked decrease in relative water contents (RWC), chlorophyll contents and yield, along with a significant increase in MDA content. Meanwhile, an obvious transcriptional inhibition in psbA gene of photosystem II (PSII) system after PEG exposure was also determined by reverse transcriptional semiquantitative polymerase chain amplification assay. The exogenous ABA treatment could significantly alleviate the stress injury. Moreover, compared to Yumai 949, Shanmai 5 had less destroyed plasma membranes, less reduces in RWC, chlorophyll contents, yield and psbA transcriptional level, and all parameters could be restored to the level of control by exogenous ABA treatment, suggesting a correlationship between cultivar dependent drought stress resistance and psbA gene expression. The present results firstly provide some evidences to the important regulatory function of exogenous ABA on psbA expression, and proposed that the application of exogenous ABA could enhance the stress resistance of wheat plant in grain filling stage by protecting important gene transcription in PSII system.

Key words: Triticum aestivum L., Abscisic acid, Grain filling stage, psbA gene expression, Drought stress

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