Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (08): 1372-1377.doi: 10.3724/SP.J.1006.2011.01372

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Effect of Abscisic Acid Treatment on psbA Gene Expression in Two Wheat Cultivars during Grain Filling Stage under Drought Stress

WANG Yue-Xia1,SUO Biao2,ZHAO Teng-Fei1,QU Xiao-Fei1,YUAN Li-Gang1,ZHAO Xue-Juan1,ZHAO Hui-Jie1,*   

  1. 1 College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China; 2 College of Food Science & Technology, Henan Agricultural University, Zhengzhou 450002, China
  • Received:2011-02-28 Revised:2011-04-26 Online:2011-08-12 Published:2011-06-13
  • Contact: 赵会杰, E-mail: zhaohj303@163.com, Tel: 0371-63555319

PDF

1885

Cited

7

Abstract: Abscisic acid (ABA) is an important plant hormone and may be involved in drought resistance in crops. The effects of exogenous ABA application on wheat plant (Triticum aestivum L.) under drought stress were investigated. When two wheat cultivars, Yumai 949 and Shanmai 5 in grain filling stage were drought stressed by PEG for 72 h, the results showed a marked decrease in relative water contents (RWC), chlorophyll contents and yield, along with a significant increase in MDA content. Meanwhile, an obvious transcriptional inhibition in psbA gene of photosystem II (PSII) system after PEG exposure was also determined by reverse transcriptional semiquantitative polymerase chain amplification assay. The exogenous ABA treatment could significantly alleviate the stress injury. Moreover, compared to Yumai 949, Shanmai 5 had less destroyed plasma membranes, less reduces in RWC, chlorophyll contents, yield and psbA transcriptional level, and all parameters could be restored to the level of control by exogenous ABA treatment, suggesting a correlationship between cultivar dependent drought stress resistance and psbA gene expression. The present results firstly provide some evidences to the important regulatory function of exogenous ABA on psbA expression, and proposed that the application of exogenous ABA could enhance the stress resistance of wheat plant in grain filling stage by protecting important gene transcription in PSII system.

Key words: Triticum aestivum L., Abscisic acid, Grain filling stage, psbA gene expression, Drought stress

[1]Chaves M M, Oliveira M M. Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture. J Exp Bot, 2004, 55: 2365–2384
[2]He J, An L, Lin H, Liang H. Evidence for transcriptional and post-transcriptional control of protein synthesis in water-stressed wheat leaves: a quantitative analysis of messenger and ribosomal RNA. J Plant Physiol, 1999, 155: 63–69
[3]Pandey D M, Yeo U D. Stress-induced degradation of D1 protein and its photoprotection by DCPIP in isolated thylakoid membranes of barley leaf. Biol Plant, 2008, 52: 291–298
[4]Leucci M R, Lenucci M S, Piro G, Dalessandro G. Water stress and cell wall polysaccharides in the apical root zone of wheat cultivars varying in drought tolerance. J Plant Physiol, 2008, 165: 1168–1180
[5]Liu W J, Yuan S, Zhang N H, Lei T, Duan H G, Liang H G, Lin H H. Effect of water stress on photosystem 2 in two wheat cultivars. Biol Plant, 2006, 50: 597–602
[6]Selote D S, Khanna-Chopra R. Antioxidant response of wheat roots to drought acclimation. Protoplasma, 2010, 245: 153-163
[7]Yamamoto Y. Quality control of photosystem II. Plant Cell Physiol, 2001, 42: 121–128
[8]Travaglia C, Cohen A C, Reinoso H, Castillo C, Bottini R. Exogenous abscisic acid increases carbohydrate accumulation and redistribution to the grains in wheat grown under field conditions of soil water restriction. J Plant Growth Regul, 2007, 26: 285–289
[9]Xue-Xuan X, Hong-Bo S, Yuan-Yuan M, Gang X, Jun-Na S, Dong-Gang G, Cheng-Jiang R. Biotechnological implications from abscisic acid (ABA) roles in cold stress and leaf senescence as an important signal for improving plant sustainable survival under abiotic-stressed conditions. Crit Rev Biotechnol, 2010, 30: 222–230
[10]Nayyar H, Kaushal S K. Alleviation of negative effects of water stress in two contrasting wheat genotypes by calcium and abscisic acid. Biol Plant, 2002, 45: 65–70
[11]Travaglia C, Reinoso H, Cohen A, Luna C, Tommasino E, Castillo C, Bottini R. Exogenous ABA increases yield in field-grown wheat with moderate water restriction. J Plant Growth Regul, 2010, 29: 366–374
[12]Vaseva I I, Grigorova B S, Simova-Stoilova L P, Demirevska K N, Feller U. Abscisic acid and late embryogenesis abundant protein profile changes in winter wheat under progressive drought stress. Plant Biol, 2010, 12: 698–707
[13]Simova-Stoilova L, Demirevska K, Petrova T, Tsenov N, Feller U. Antioxidative protection and proteolytic activity in tolerant and sensitive wheat (Triticum aestivum L.) varieties subjected to long-term field drought. Plant Growth Regul, 2009, 58: 107–117
[14]Sangtarash M H. Responses of different wheat genotypes to drought stress applied at different growth stages. Pak J Biol Sci, 2010, 13: 114-119
[15]Madani A, Rad A S, Pazoki A, Nourmohammadi G, Zarghami R. Wheat (Triticum aestivum L.) grain filling and dry matter partitioning responses to source:sink modifications under postanthesis water and nitrogen deficiency. Acta Sci Agron, 2010, 32: 145–151
[16]Wei T-M(魏添梅), Chang X-P(昌小平), Min D-H(闵东红), Jing R-L(景蕊莲). Analysis of genetic diversity and trapping elite alleles for plant height in drought-tolerant wheat cultivars. Acta Agron Sin (作物学报), 2010, 36(6): 895–904 (in Chinese with English abstract)
[17]Schonfeld M, Johnson R, Carver B, Mornhinweg D. Water relations in winter wheat as drought resistant indicators. Crop Sci, 1988, 28: 526–531
[18]Phan C, Letouze R. A comparative study of chlorophyll, phenolic and protein contents, and of hydroxycinnamate: CoA ligase activity of normal and ‘vitreous’ plants (Prunus avium L.) obtained in vitro. Plant Sci Lett, 1983, 31: 323–327
[19]Wang Y X, Sun G R, Suo B, Chen G, Wang J B, Yan Y. Effects of Na2CO3 and NaCl stresses on the antioxidant enzymes of chloroplasts and chlorophyll fluorescence parameters of leaves of Puccinellia tenuiflora (Turcz.) Scribn.et Merr. Acta Physiol Plant, 2008, 30: 143–150
[20]Xuan W, Huang L, Li M, Huang B, Xu S, Liu H, Gao Y, Shen W. Induction of growth elongation in wheat root segments by heme molecules: a regulatory role of carbon monoxide in plants? Plant Growth Regul, 2007, 52: 41-51
[21]Hsiao T. Plant responses to water stress. Annu Rev Plant Physiol, 1973, 24: 519–570
[22]Sharma P K, Singhal G S. Effect of water stress on primary photosynthetic processes: interaction with light and temperature. Indian J Biochem Biophys, 1993; 30: 10–14
[23]Wang G P, Hui Z, Li F, Zhao M R, Zhang J, Wang W. Improvement of heat and drought photosynthetic tolerance in wheat by overaccumulation of glycinebetaine. Plant Biotechnol Rep, 2010, 4: 213–222
[24]Yuan S, Liu W J, Zhang N H, Wang M B, Liang H G, Lin H H. Effects of water stress on major photosystem II gene expression and protein metabolism in barley leaves. Physiol Plant, 2005, 125: 464–473
[25]Edelman M, Mattoo A K. D1-protein dynamics in photosystem II: the lingering enigma. Photosynth Res, 2008, 98: 609–620
[26]Mulo P, Sicora C, Aro E M. Cyanobacterial psbA gene family: optimization of oxygenic photosynthesis. Cell Mol Life Sci, 2009, 66: 3697–3710
[27]Zhang J H, Jia W S, Yang J C, Ismail A M. Role of ABA in integrating plant responses to drought and salt stresses. Field Crops Res, 2006, 97: 111–119
[28]Huang D Q, Wu W R, Abrams S R, Cutler A J. The relationship of drought-related gene expression in Arabidopsis thaliana to hormonal and environmental factors. J Exp Bot, 2008, 59: 2991–3007
[29]Krugman T, Chague V, Peleg Z, Balzergue S, Just J, Korol A B, Nevo E, Saranga Y, Chalhoub B, Fahima T. Multilevel regulation and signalling processes associated with adaptation to terminal drought in wild emmer wheat. Funct Integr Genomic, 2010, 10: 167–186
[30]Bright J, Desikan R, Hancock J T, Weir I S, Neill S J. ABA-induced NO generation and stomatal closure in Arabidopsis are dependent on H2O2 synthesis. Plant J, 2006, 45: 113–122
[1] WANG Xia, YIN Xiao-Yu, Yu Xiao-Ming, LIU Xiao-Dan. Effects of drought hardening on contemporary expression of drought stress memory genes and DNA methylation in promoter of B73 inbred progeny [J]. Acta Agronomica Sinica, 2022, 48(5): 1191-1198.
[2] DING Hong, XU Yang, ZHANG Guan-Chu, QIN Fei-Fei, DAI Liang-Xiang, ZHANG Zhi-Meng. Effects of drought at different growth stages and nitrogen application on nitrogen absorption and utilization in peanut [J]. Acta Agronomica Sinica, 2022, 48(3): 695-703.
[3] ZHANG Ming-Cong, HE Song-Yu, QIN Bin, WANG Meng-Xue, JIN Xi-Jun, REN Chun-Yuan, WU Yao-Kun, ZHANG Yu-Xian. Effects of exogenous melatonin on morphology, photosynthetic physiology, and yield of spring soybean variety Suinong 26 under drought stress [J]. Acta Agronomica Sinica, 2021, 47(9): 1791-1805.
[4] LI Jie, FU Hui, YAO Xiao-Hua, WU Kun-Lun. Differentially expressed protein analysis of different drought tolerance hulless barley leaves [J]. Acta Agronomica Sinica, 2021, 47(7): 1248-1258.
[5] LI Peng-Cheng, BI Zhen-Zhen, SUN Chao, QIN Tian-Yuan, LIANG Wen-Jun, WANG Yi-Hao, XU De-Rong, LIU Yu-Hui, ZHANG Jun-Lian, BAI Jiang-Ping. Key genes mining of DNA methylation involved in regulating drought stress response in potato [J]. Acta Agronomica Sinica, 2021, 47(4): 599-612.
[6] QIN Tian-Yuan, LIU Yu-Hui, SUN Chao, BI Zhen-Zhen, LI An-Yi, XU De-Rong, WANG Yi-Hao, ZHANG Jun-Lian, BAI Jiang-Ping. Identification of StIgt gene family and expression profile analysis of response to drought stress in potato [J]. Acta Agronomica Sinica, 2021, 47(4): 780-786.
[7] ZHOU Lian, LIU Chao-Xian, XIONG Yu-Han, ZHOU Jing, CAI Yi-Lin. Functional analysis of plasma membrane intrinsic protein ZmPIP1;1 involved in drought tolerance and photosynthesis in maize [J]. Acta Agronomica Sinica, 2021, 47(3): 472-480.
[8] LIU Ya-Wen, ZHANG Hong-Yan, CAO Dan, LI Lan-Zhi. Prediction of drought and salt stress-related genes in rice based on multi-platform gene expression data [J]. Acta Agronomica Sinica, 2021, 47(12): 2423-2439.
[9] QIN Tian-Yuan, SUN Chao, BI Zhen-Zhen, LIANG Wen-Jun, LI Peng-Cheng, ZHANG Jun-Lian, BAI Jiang-Ping. Identification of drought-related co-expression modules and hub genes in potato roots based on WGCNA [J]. Acta Agronomica Sinica, 2020, 46(7): 1033-1051.
[10] ZHANG Hai-Yan, WANG Bao-Qing, FENG Xiang-Yang, LI Guang-Liang, XIE Bei-Tao, DONG Shun-Xu, DUAN Wen-Xue, ZHANG Li-Ming. Effects of drought treatments at different growth stages on growth and the activity of osmotic adjustment in sweet potato [Ipomoea batatas (L.) Lam.] [J]. Acta Agronomica Sinica, 2020, 46(11): 1760-1770.
[11] LI Xu-Kai,LI Ren-Jian,ZHANG Bao-Jun. Identification of rice stress-related gene co-expression modules by WGCNA [J]. Acta Agronomica Sinica, 2019, 45(9): 1349-1364.
[12] ZHANG Cheng-Xin,GUO Bao-Wei,TANG Jian,XU Fang-Fu,XU Ke,HU Ya-Jie,XING Zhi-Peng,ZHANG Hong-Cheng,DAI Qi-Gen,HUO Zhong-Yang,WEI Hai-Yan,HUANG Li-Fen,LU Yang,TANG Chuang,DAI Qi-Xing,ZHOU Miao,SUN Jun-Yi. Combined effects of low temperature and weak light at grain-filling stage on rice grain quality [J]. Acta Agronomica Sinica, 2019, 45(8): 1208-1220.
[13] SONG Song-Quan,LIU Jun,XU Heng-Heng,ZHANG Qi,HUANG Hui,WU Xian-Jin. Biosynthesis and signaling of ethylene and their regulation on seed germination and dormancy [J]. Acta Agronomica Sinica, 2019, 45(7): 969-981.
[14] Yi YUAN,Shuang ZHU,Ting-Ting FANG,Jin-Jin JIANG,You-Ping WANG. Analysis of drought resistance and DNA methylation level of resynthesized Brassica napus [J]. Acta Agronomica Sinica, 2019, 45(5): 693-704.
[15] Ping LI,Wan-Wei HOU,Yu-Jiao LIU. Proteomic analysis of drought stress response on drought resistance for Vicia faba L. variety ‘Qinghai 13’ in Qinghai Plateau of China [J]. Acta Agronomica Sinica, 2019, 45(2): 267-275.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd