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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (01): 182-189.doi: 10.3724/SP.J.1006.2011.00182

• RESEARCH ACTIVITIES • Previous Articles    

Effects of Soil Moisture after Anthesis on Isozymes of Glutamine Synthetase, Grain Yield, and Protein Content in Winter Wheat Yumai 34

XIONG Shu-Ping1,WANG Xiao-Chun2,MA Xin-Ming1,*,ZHAO Peng1,WANG Lu1,CHENG Zhen-Yun2   

  1. 1College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China; 2College of Life Science, Henan Agricultural University, Zhengzhou 450002, China
  • Received:2010-06-19 Revised:2010-08-17 Online:2011-01-12 Published:2010-11-16
  • Contact: MA Xin-Ming,E-mail:xinmingma@126.com

Abstract: In a two-year experiment, the strong-gluten wheat cultivar, Yumai 34, were planted with three treatments, i.e., 40% (40%FC), 60% (60%FC), and 80% (80%FC) of field water holding capacity. The activities of GS and GS isozymes and the soluble protein contents in flag leaf and grain were measured after anthesis. The results showed that GS activity in flag leaf was more than higher than that in grain, and decreased gradually after anthesis. Isozymes GS1, GS2, and GSx were identified in flag leaf, of which GS2 had the highest activity. Only GS1 was observed in grain. The effects of water treatment on activities of GS and GS isozymes both ranked as 60%FC>80%FC>40%FC, and was largest for GS2 in flag leaf. The content of soluble protein in flag leaf ranked as 60%FC>80%FC>40%FC at early stage of grain filling and as 80%FC>60%FC>40%FC afterwards. The content of soluble protein in grain ranked as 80%FC>60%FC>40%FC. In the 60%FC treatment, the content of soluble protein in flag leaf was positively correlated with the activities of GS and GS2 in flag leaf. The highest grain yield and protein content were observed in the 60%FC treatment, indicating that 60% of field water holding capacity was the optimal soil moisture for Yumai 34 growth after anthesis.

Key words: Soil moisture, Isozymes of glutamine synthetase, Soluble protein, Grain yield

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