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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (06): 955-964.doi: 10.3724/SP.J.1006.2011.00955

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification and Fine Mapping of a Semidwarf Gene iga-1 in Rice

GUO Tao,HUO Xing**,RAO De-Hua,LIU Yong-Zhu,ZHANG Jian-Guo,CHEN Zhi-Qiang,WANG Hui*   

  1. South China Agricultural University, National Engineering Research Center of Plant Space Breeding, Guangzhou 510642, China
  • Received:2010-10-27 Revised:2010-03-06 Online:2011-06-12 Published:2011-04-12

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Abstract: A semidwarf gene iga-1 of rice (Oryza sativa L.) by mutagenesis of outer space treatment from Texianzhan 13 was identified. The dwarf lines CHA-2 and CHA-2N which carried iga-1 showed great variation in agronomic traits. On the basis of the internode length of CHA-2 and CHA-2N, the mutant belongs to the dn type of dwarfing. GA3 treatment, endo-GA3 measurement and α-amylase activity analysis in endosperm showed that iga-1 is independent of gibberellin acid. Using a large F2 population derived from a cross between the CHA-2 and an japonica rice variety, 02428, the iga-1 gene was fine mapped into a 32.01 kb physical distance between two InDel markers, DL18 and DL19 on chromosome 5, where five open reading frames were predicted, one of which was the rice gibberellin-insensitive dwarf mutant gene D1. Sequence analysis showed that no variation in D1 locus was detected among CHA-2, CHA-2N and Texianzhan 13. Thus, D1 can not be the candidate gene of iga-1. Comparing the other dwarf genes on chromosome 5 showed that iga-1 is possibly allelic to the semidwarf gene sd-7.

Key words: Rice, Semidwarf mutant, iga-1, Fine mapping

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