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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (12): 2299-2305.doi: 10.3724/SP.J.1006.2011.02299

• RESEARCH NOTES • Previous Articles    

Cotton Hybrid Purity Tested by SSR Markers and Its Correlation with Phenotype Identification in Field

KUANG Meng,YANG Wei-Hua*,ZHANG Yu-Cui,XU Hong-Xia,WANG Yan-Qin,ZHOU Da-Yun,FENG Xin-Ai,SU Chang,ZHOU Hong   

  1. Cotton Research Institute, Chinese Academy of Agriculture Sciences / State Key Laboratory of Cotton Biology, Anyang, 455000, China
  • Received:2011-06-13 Revised:2011-09-18 Online:2011-12-12 Published:2011-09-29
  • Contact: 杨伟华, E-mail: yangwh @cricaas.com.cn, Tel: 0372-2525389

Abstract: Cotton is one of the most important cash corps. Hybrid cotton is extensively grown in China. Hybrid seeds are generally planted and used for purity identification. The processtakes long time and much money. Therefore, it is important to explore a new method to inspect the purity of hybrid cotton. In order to explore the validity of hybrid cotton purity identification using SSR marker technique, the purity for six hybrids was tested by SSR approach and morphological assessment, and the correlation between the two methods was researched. Primers with complementary band type screened out from 36 evenly distributed SSR pairs were applied in purity identification. Morphological traits including plant type, boll, leaf shape, flower and stem were observed. Two statistics methods were used for testingpurity based on SSR marker, and the results were compared to that of morphological identification. Among the six hybrid cottons, 34 primer pairs had 101 heterozygous loci, with an average of 16.8 heterozygous loci for on each hybrid. The purity based on morphology was higher than that on SSR markers, the results of “Single locus on average” (the average value of purity test by each SSR locus) had a higher correlation with morphology compared to “Double locus difference” (if more than two SSR loci of an individual are different from the tested cultivar’s, the individual is considered as farraginous plant), and the correlation would be better after calibration (correlation coefficient reached to 0.7841). Correlation based on EST-SSR was much better than that on Genomic-SSR. There was certain relativity at different loci of the same chromosome. The purity was relevant to different statistics method and marker type. There will be a good correlation for purity identification between methods of SSR marker and morphology if the hybrid parents are highly pure.

Key words: Cotton, Hybrid, SSR, Morphology, Purity

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