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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (01): 186-189.doi: 10.3724/SP.J.1006.2012.00186

• RESEARCH NOTES • Previous Articles    

Establishment of a HPLC-UV Method for Simultaneous Determination of DON, 15ACDON, and 3ACDON in Wheat

YANG Dan,GENG Zhi-Ming,MA Hong-Xiang,YAO Jin-Bao,ZHANG Xu,ZHANG Ping-Ping,ZHANG Peng   

  1. Provincial Key Laboratory of Agrobiology / Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2011-05-27 Revised:2011-09-18 Online:2012-01-12 Published:2011-09-30
  • Contact: 马鸿翔, E-mail: mahx@jaas.ac.cn

Abstract: A HPLC-UV method was established to determine deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ACDON) and 15- acetyldeoxynivalenol (15-ACDON) in wheat. Wheat sample was extracted by water, and the extracting solution was precipitated with an equal volume of ethanol followed by solid phase extraction with Oasis HLB cartridge. Qualitative and quantitative analyses of DON, 15ACDON and 3ACDON were done by HPLC-UVD using gradient elution program with acetonitrile and 0.005% phosphoric acid solution. Results showed that at the linear range of 0.5–15.0 mg L-1, the recoveries were 89.8%, 93.4%, and 92.9%, respectively. The relative standard deviations were 2.2%, 2.0%, and 2.5%, respectively, and the detection limit levels were 12.2, 10.5, and 16.7 μg kg-1, respectively. The method was accurate and reproducible. Sample purification lessened interference peaks, and due to the use of organic solvents was greatly reduced, the cost was decreased. Therefore, the method was suitable for the large batch determination of type B trichothecenes in wheat.

Key words: Wheat, B trichothecenes, HPLC-UV

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