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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (02): 333-343.doi: 10.3724/SP.J.1006.2012.00333

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effect of Wheat/Maize/Soybean and Wheat/Maize/Sweet Potato Relay Strip Intercropping on Bacterial Community Diversity of Rhizosphere Soil and Nitrogen Uptake of Crops

YONG Tai-Wen,YANG Wen-Yu*,XIANG Da-Bing,ZHU Zhen-Ying   

  1. Agronomy College, Sichuan Agricultural University, Wenjiang 611130, China
  • Received:2011-04-08 Revised:2011-09-11 Online:2012-02-12 Published:2011-12-01
  • Contact: 杨文钰, E-mail: wenyu.yang@263.net

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Abstract: The aim of this study was to understand the relationship between diversity of rhizosphere bacterial community and nitrogen uptake of crops in two relay strip intercropping systems: wheat/maize/soybean and wheat/maize/sweet potato. We analyzed the diversities of rhizosphere bacterial community in five cropping systems using denaturing gradient gel electrophoresis (DGGE) based on 16S rDNA. The cropping systems included wheat-soybean (A1), wheat-sweet potato (A2), maize single cropping (A3), wheat/maize/soybean (A4), and wheat/maize/sweet potato (A5). Compared to the sole cropping systems (A1, A2, and A3 treatments), the A4 treatment showed increases in grain nitrogen uptake and total nitrogen uptake amounts of aboveground of crops at both flowering (or silking) and maturity stages, and the Shannon-Weiner indices for rhizosphere bacterial community diversity was also increased significantly. The values of nitrogen uptake and the Shannon-weiner index in the relay strip intercropping systems were greater than those in sole cropping systems, and the values in the treatment with soybean stubble were greater than those in the treatment with sweet potato stubble. The number and strength of the DNA bands from DGGE profiles varied with the cropping systems, especially for the distinguished bands. The comparability of rhizosphere bacterial community composition was low among different cropping systems. The similarity coefficient (Cs) was higher between two relay strip intercropping systems than between relay strip intercropping system and sole cropping system, but the Cs value was low between A4 and A5 treatments. However, there was obvious difference of rhizosphere bacterial community composition between A4 and A5 treatments, and the A4 treatment was propitious to enhancing rhizosphere bacterial community diversity and increasing nitrogen uptakes of crops.

Key words: Wheat/maize/soybean relay strip intercropping, Nitrogen uptake, Bacterial community diversity, PCR-DGGE

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